LOCUS       sequence-464829-        1745 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Term_bla-v2_PartType10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK109 [Term_bla-v2_PartType10]
FEATURES             Location/Qualifiers
     source          1..1745
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      623..662
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     promoter        723..814
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(815..883,884..1675)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1679..1718
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctatcta ccgatacaat taaaggctcc ttttggagcc tttttttttg
      661 gacgacccta catgagacca gagtagcccg cctaatgagc gggctttttt ttaattcccc
      721 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      781 ataaatgctt caataatatt gaaaaaggaa gagtatgagc attcagcatt ttcgtgtggc
      841 gctgattccg ttttttgcgg cgttttgcct gccggtgttt gcgcatccgg aaaccctggt
      901 gaaagtgaaa gatgcggaag atcaactggg tgcgcgcgtg ggctatattg aactggatct
      961 gaacagcggc aaaattctgg aatcttttcg tccggaagaa cgttttccga tgatgagcac
     1021 ctttaaagtg ctgctgtgcg gtgcggttct gagccgtgtg gatgcgggcc aggaacaact
     1081 gggccgtcgt attcattata gccagaacga tctggtggaa tatagcccgg tgaccgaaaa
     1141 acatctgacc gatggcatga ccgtgcgtga actgtgcagc gcggcgatta ccatgagcga
     1201 taacaccgcg gcgaacctgc tgctgacgac cattggcggt ccgaaagaac tgaccgcgtt
     1261 tctgcataac atgggcgatc atgtgacccg tctggatcgt tgggaaccgg aactgaacga
     1321 agcgattccg aacgatgaac gtgataccac catgccggca gcaatggcga ccaccctgcg
     1381 taaactgctg acgggtgagc tgctgaccct ggcaagccgc cagcaactga ttgattggat
     1441 ggaagcggat aaagtggcgg gtccgctgct gcgtagcgcg ctgccggctg gctggtttat
     1501 tgcggataaa agcggtgcgg gcgaacgtgg cagccgtggc attattgcgg cgctgggccc
     1561 ggatggtaaa ccgagccgta ttgtggtgat ttataccacc ggcagccagg cgacgatgga
     1621 tgaacgtaac cgtcagattg cggaaattgg cgcgagcctg attaaacatt ggtaaaccga
     1681 tacaattaaa ggctcctttt ggagcctttt tttttggacg acccttgtca gatcaaagga
     1741 tcttc
//
