LOCUS       sequence-464833-        1722 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Term_neo_PartType10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK113 [Term_neo_PartType10]
FEATURES             Location/Qualifiers
     source          1..1722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        700..791
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(792..860,861..1652)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1656..1695
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctatctt aattaattgg accgcggtcc gcgcgtacat gagaccagag
      661 tagcccgcct aatgagcggg ctttttttta attcccctat ttgtttattt ttctaaatac
      721 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      781 aaaggaagag tatgagcatt cagcattttc gtgtggcgct gattccgttt tttgcggcgt
      841 tttgcctgcc ggtgtttgcg catccggaaa ccctggtgaa agtgaaagat gcggaagatc
      901 aactgggtgc gcgcgtgggc tatattgaac tggatctgaa cagcggcaaa attctggaat
      961 cttttcgtcc ggaagaacgt tttccgatga tgagcacctt taaagtgctg ctgtgcggtg
     1021 cggttctgag ccgtgtggat gcgggccagg aacaactggg ccgtcgtatt cattatagcc
     1081 agaacgatct ggtggaatat agcccggtga ccgaaaaaca tctgaccgat ggcatgaccg
     1141 tgcgtgaact gtgcagcgcg gcgattacca tgagcgataa caccgcggcg aacctgctgc
     1201 tgacgaccat tggcggtccg aaagaactga ccgcgtttct gcataacatg ggcgatcatg
     1261 tgacccgtct ggatcgttgg gaaccggaac tgaacgaagc gattccgaac gatgaacgtg
     1321 ataccaccat gccggcagca atggcgacca ccctgcgtaa actgctgacg ggtgagctgc
     1381 tgaccctggc aagccgccag caactgattg attggatgga agcggataaa gtggcgggtc
     1441 cgctgctgcg tagcgcgctg ccggctggct ggtttattgc ggataaaagc ggtgcgggcg
     1501 aacgtggcag ccgtggcatt attgcggcgc tgggcccgga tggtaaaccg agccgtattg
     1561 tggtgattta taccaccggc agccaggcga cgatggatga acgtaaccgt cagattgcgg
     1621 aaattggcgc gagcctgatt aaacattggt aaaccgatac aattaaaggc tccttttgga
     1681 gccttttttt ttggacgacc cttgtcagat caaaggatct tc
//
