LOCUS       sequence-464834-        1799 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Term_T0_PartType10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK114 [Term_T0_PartType10]
FEATURES             Location/Qualifiers
     source          1..1799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      624..718
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        777..868
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(869..937,938..1729)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1733..1772
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctatctc ttggactcct gttgatagat ccagtaatga cctcagaact
      661 ccatctggat ttgttcagaa cgctcggttg ccgccgggcg ttttttattg gtgagaatcc
      721 agtacatgag accagagtag cccgcctaat gagcgggctt ttttttaatt cccctatttg
      781 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      841 gcttcaataa tattgaaaaa ggaagagtat gagcattcag cattttcgtg tggcgctgat
      901 tccgtttttt gcggcgtttt gcctgccggt gtttgcgcat ccggaaaccc tggtgaaagt
      961 gaaagatgcg gaagatcaac tgggtgcgcg cgtgggctat attgaactgg atctgaacag
     1021 cggcaaaatt ctggaatctt ttcgtccgga agaacgtttt ccgatgatga gcacctttaa
     1081 agtgctgctg tgcggtgcgg ttctgagccg tgtggatgcg ggccaggaac aactgggccg
     1141 tcgtattcat tatagccaga acgatctggt ggaatatagc ccggtgaccg aaaaacatct
     1201 gaccgatggc atgaccgtgc gtgaactgtg cagcgcggcg attaccatga gcgataacac
     1261 cgcggcgaac ctgctgctga cgaccattgg cggtccgaaa gaactgaccg cgtttctgca
     1321 taacatgggc gatcatgtga cccgtctgga tcgttgggaa ccggaactga acgaagcgat
     1381 tccgaacgat gaacgtgata ccaccatgcc ggcagcaatg gcgaccaccc tgcgtaaact
     1441 gctgacgggt gagctgctga ccctggcaag ccgccagcaa ctgattgatt ggatggaagc
     1501 ggataaagtg gcgggtccgc tgctgcgtag cgcgctgccg gctggctggt ttattgcgga
     1561 taaaagcggt gcgggcgaac gtggcagccg tggcattatt gcggcgctgg gcccggatgg
     1621 taaaccgagc cgtattgtgg tgatttatac caccggcagc caggcgacga tggatgaacg
     1681 taaccgtcag attgcggaaa ttggcgcgag cctgattaaa cattggtaaa ccgatacaat
     1741 taaaggctcc ttttggagcc tttttttttg gacgaccctt gtcagatcaa aggatcttc
//
