LOCUS       sequence-464837-        1855 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Term_tetA_PartType10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK117 [Term_tetA_PartType10]
FEATURES             Location/Qualifiers
     source          1..1855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        833..924
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(925..993,994..1785)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1789..1828
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctatctt cgtggaaacg ataggcctat gccatgcggg tcaaggcgac
      661 ttccggcaag ctatacgcgc cctaggagtg cggttggaac gttggcccag ccagatactc
      721 ccgatcacga gcaggacgcc gatgatttga agcgcactca gcgtctgatc ttaattaata
      781 catgagacca gagtagcccg cctaatgagc gggctttttt ttaattcccc tatttgttta
      841 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
      901 caataatatt gaaaaaggaa gagtatgagc attcagcatt ttcgtgtggc gctgattccg
      961 ttttttgcgg cgttttgcct gccggtgttt gcgcatccgg aaaccctggt gaaagtgaaa
     1021 gatgcggaag atcaactggg tgcgcgcgtg ggctatattg aactggatct gaacagcggc
     1081 aaaattctgg aatcttttcg tccggaagaa cgttttccga tgatgagcac ctttaaagtg
     1141 ctgctgtgcg gtgcggttct gagccgtgtg gatgcgggcc aggaacaact gggccgtcgt
     1201 attcattata gccagaacga tctggtggaa tatagcccgg tgaccgaaaa acatctgacc
     1261 gatggcatga ccgtgcgtga actgtgcagc gcggcgatta ccatgagcga taacaccgcg
     1321 gcgaacctgc tgctgacgac cattggcggt ccgaaagaac tgaccgcgtt tctgcataac
     1381 atgggcgatc atgtgacccg tctggatcgt tgggaaccgg aactgaacga agcgattccg
     1441 aacgatgaac gtgataccac catgccggca gcaatggcga ccaccctgcg taaactgctg
     1501 acgggtgagc tgctgaccct ggcaagccgc cagcaactga ttgattggat ggaagcggat
     1561 aaagtggcgg gtccgctgct gcgtagcgcg ctgccggctg gctggtttat tgcggataaa
     1621 agcggtgcgg gcgaacgtgg cagccgtggc attattgcgg cgctgggccc ggatggtaaa
     1681 ccgagccgta ttgtggtgat ttataccacc ggcagccagg cgacgatgga tgaacgtaac
     1741 cgtcagattg cggaaattgg cgcgagcctg attaaacatt ggtaaaccga tacaattaaa
     1801 ggctcctttt ggagcctttt tttttggacg acccttgtca gatcaaagga tcttc
//
