LOCUS       sequence-464838-        1874 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Term_tetR_PartType10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK118 [Term_tetR_PartType10]
FEATURES             Location/Qualifiers
     source          1..1874
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        852..943
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(944..1012,1013..1804)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1808..1847
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctatctt caggaccgct gccggagcgc aacccactca ctacagcaga
      661 gccatgtaga caacatcccc tccccctttc caccgcgtca gacgcccgta gcagcccgct
      721 acgggctttt tcatgccctg ccctagcgtc caagcctcac ggccgcgctc ggcctctctg
      781 gcggccttct ggcgctctac atgagaccag agtagcccgc ctaatgagcg ggcttttttt
      841 taattcccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      901 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagca ttcagcattt
      961 tcgtgtggcg ctgattccgt tttttgcggc gttttgcctg ccggtgtttg cgcatccgga
     1021 aaccctggtg aaagtgaaag atgcggaaga tcaactgggt gcgcgcgtgg gctatattga
     1081 actggatctg aacagcggca aaattctgga atcttttcgt ccggaagaac gttttccgat
     1141 gatgagcacc tttaaagtgc tgctgtgcgg tgcggttctg agccgtgtgg atgcgggcca
     1201 ggaacaactg ggccgtcgta ttcattatag ccagaacgat ctggtggaat atagcccggt
     1261 gaccgaaaaa catctgaccg atggcatgac cgtgcgtgaa ctgtgcagcg cggcgattac
     1321 catgagcgat aacaccgcgg cgaacctgct gctgacgacc attggcggtc cgaaagaact
     1381 gaccgcgttt ctgcataaca tgggcgatca tgtgacccgt ctggatcgtt gggaaccgga
     1441 actgaacgaa gcgattccga acgatgaacg tgataccacc atgccggcag caatggcgac
     1501 caccctgcgt aaactgctga cgggtgagct gctgaccctg gcaagccgcc agcaactgat
     1561 tgattggatg gaagcggata aagtggcggg tccgctgctg cgtagcgcgc tgccggctgg
     1621 ctggtttatt gcggataaaa gcggtgcggg cgaacgtggc agccgtggca ttattgcggc
     1681 gctgggcccg gatggtaaac cgagccgtat tgtggtgatt tataccaccg gcagccaggc
     1741 gacgatggat gaacgtaacc gtcagattgc ggaaattggc gcgagcctga ttaaacattg
     1801 gtaaaccgat acaattaaag gctccttttg gagccttttt ttttggacga cccttgtcag
     1861 atcaaaggat cttc
//
