LOCUS       sequence-464841-        3074 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Misc_CEN6-ARS4-HIS3_PartType11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK121 [Misc_CEN6-ARS4-HIS3_PartType11]
FEATURES             Location/Qualifiers
     source          1..3074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    620..1123
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        1124..1310
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             1311..1973
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        2052..2143
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2144..2212,2213..3004)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      3008..3047
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctcttacaa tcacgtgcta taaaaataat tataatttaa attttttaat
      661 ataaatatat aaattaaaaa tagaaagtaa aaaaagaaat taaagaaaaa atagtttttg
      721 ttttccgaag atgtaaaaga ctctaggggg atcgccaaca aatactacct tttaccttgc
      781 tcttcctgct ctcaggtatt aatgccgaat tgtttcatct tgtctgtgta gaagaccaca
      841 cacgaaaatc ctgtgatttt acattttact tatcgttaat cgaatgtata tctatttaat
      901 ctgcttttct tgtctaataa atatatatgt aaagtacgct ttttgttgaa attttttaaa
      961 cctttgttta tttttttttc ttcattccgt aactcttcta ccttctttat ttactttcta
     1021 aaatccaaat acaaaacata aaaataaata aacacagagt aaattcccaa attattccat
     1081 cattaaaaga tacgaggcgc gtgtaagtta caggcaagcg atcctagtac actctatatt
     1141 tttttatgcc tcggtaatga ttttcatttt ttttttccac ctagcggatg actctttttt
     1201 tttcttagcg attggcatta tcacataatg aattatacat tatataaagt aatgtgattt
     1261 cttcgaagaa tatactaaaa aatgagcagg caagataaac gaaggcaaag atgacagagc
     1321 agaaagccct agtaaagcgt attacaaatg aaaccaagat tcagattgcg atctctttaa
     1381 agggtggtcc cctagcgata gagcactcga tcttcccaga aaaagaggca gaagcagtag
     1441 cagaacaggc cacacaatcg caagtgatta acgtccacac aggtataggg tttctggacc
     1501 atatgataca tgctctggcc aagcattccg gctggtcgct aatcgttgag tgcattggtg
     1561 acttacacat agacgaccat cacaccactg aagactgcgg gattgctctc ggtcaagctt
     1621 ttaaagaggc cctaggggcc gtgcgtggag taaaaaggtt tggatcagga tttgcgcctt
     1681 tggatgaggc actttccaga gcggtggtag atctttcgaa caggccgtac gcagttgtcg
     1741 aacttggttt gcaaagggag aaagtaggag atctctcttg cgagatgatc ccgcattttc
     1801 ttgaaagctt tgcagaggct agcagaatta ccctccacgt tgattgtctg cgaggcaaga
     1861 atgatcatca ccgtagtgag agtgcgttca aggctcttgc ggttgccata agagaagcca
     1921 cctcgcccaa tggtaccaac gatgttccct ccaccaaagg tgttcttatg tagttttaca
     1981 caggagtctg gacttgagag ttgagaccag agtagcccgc ctaatgagcg ggcttttttt
     2041 taattcccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     2101 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagca ttcagcattt
     2161 tcgtgtggcg ctgattccgt tttttgcggc gttttgcctg ccggtgtttg cgcatccgga
     2221 aaccctggtg aaagtgaaag atgcggaaga tcaactgggt gcgcgcgtgg gctatattga
     2281 actggatctg aacagcggca aaattctgga atcttttcgt ccggaagaac gttttccgat
     2341 gatgagcacc tttaaagtgc tgctgtgcgg tgcggttctg agccgtgtgg atgcgggcca
     2401 ggaacaactg ggccgtcgta ttcattatag ccagaacgat ctggtggaat atagcccggt
     2461 gaccgaaaaa catctgaccg atggcatgac cgtgcgtgaa ctgtgcagcg cggcgattac
     2521 catgagcgat aacaccgcgg cgaacctgct gctgacgacc attggcggtc cgaaagaact
     2581 gaccgcgttt ctgcataaca tgggcgatca tgtgacccgt ctggatcgtt gggaaccgga
     2641 actgaacgaa gcgattccga acgatgaacg tgataccacc atgccggcag caatggcgac
     2701 caccctgcgt aaactgctga cgggtgagct gctgaccctg gcaagccgcc agcaactgat
     2761 tgattggatg gaagcggata aagtggcggg tccgctgctg cgtagcgcgc tgccggctgg
     2821 ctggtttatt gcggataaaa gcggtgcggg cgaacgtggc agccgtggca ttattgcggc
     2881 gctgggcccg gatggtaaac cgagccgtat tgtggtgatt tataccaccg gcagccaggc
     2941 gacgatggat gaacgtaacc gtcagattgc ggaaattggc gcgagcctga ttaaacattg
     3001 gtaaaccgat acaattaaag gctccttttg gagccttttt ttttggacga cccttgtcag
     3061 atcaaaggat cttc
//
