LOCUS       sequence-464843-        2734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Misc_Expanding-Modulator-B_PartType8-11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK123 [Misc_Expanding-Modulator-B_PartType8-11]
FEATURES             Location/Qualifiers
     source          1..2734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     RBS             783..794
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             801..1514
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1526..1597
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1613..1640
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        1712..1803
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1804..1872,1873..2664)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2668..2707
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctgctga acggtacgca ggtggaaagt gaaacgtgat ttcatgcgtc
      661 attttgaaca ttttgtaaat cttatttaat aatgtgtgcg gcaattcaca tttaatttat
      721 gaatgttttc ttaacatcgc ggcaactcaa gaaacggcag gttcggatct tagctactag
      781 agaaagagga gaaatactag atgcgtaaag gcgaagagct gttcactggt gtcgtcccta
      841 ttctggtgga actggatggt gatgtcaacg gtcataagtt ttccgtgcgt ggcgagggtg
      901 aaggtgacgc aactaatggt aaactgacgc tgaagttcat ctgtactact ggtaaactgc
      961 cggttccttg gccgactctg gtaacgacgc tgacttatgg tgttcagtgc tttgctcgtt
     1021 atccggacca tatgaagcag catgacttct tcaagtccgc catgccggaa ggctatgtgc
     1081 aggaacgcac gatttccttt aaggatgacg gcacgtacaa aacgcgtgcg gaagtgaaat
     1141 ttgaaggcga taccctggta aaccgcattg agctgaaagg cattgacttt aaagaggacg
     1201 gcaatatcct gggccataag ctggaataca attttaacag ccacaatgtt tacatcaccg
     1261 ccgataaaca aaaaaatggc attaaagcga attttaaaat tcgccacaac gtggaggatg
     1321 gcagcgtgca gctggctgat cactaccagc aaaacactcc aatcggtgat ggtcctgttc
     1381 tgctgccaga caatcactat ctgagcacgc aaagcgttct gtctaaagat ccgaacgaga
     1441 aacgcgatca tatggttctg ctggagttcg taaccgcagc gggcatcacg catggtatgg
     1501 atgaactgta caaatgacca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1561 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     1621 ttcgggtggg cctttctgcg tttatacacc tgcgtacgag ttgagaccag agtagcccgc
     1681 ctaatgagcg ggcttttttt taattcccct atttgtttat ttttctaaat acattcaaat
     1741 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     1801 agtatgagca ttcagcattt tcgtgtggcg ctgattccgt tttttgcggc gttttgcctg
     1861 ccggtgtttg cgcatccgga aaccctggtg aaagtgaaag atgcggaaga tcaactgggt
     1921 gcgcgcgtgg gctatattga actggatctg aacagcggca aaattctgga atcttttcgt
     1981 ccggaagaac gttttccgat gatgagcacc tttaaagtgc tgctgtgcgg tgcggttctg
     2041 agccgtgtgg atgcgggcca ggaacaactg ggccgtcgta ttcattatag ccagaacgat
     2101 ctggtggaat atagcccggt gaccgaaaaa catctgaccg atggcatgac cgtgcgtgaa
     2161 ctgtgcagcg cggcgattac catgagcgat aacaccgcgg cgaacctgct gctgacgacc
     2221 attggcggtc cgaaagaact gaccgcgttt ctgcataaca tgggcgatca tgtgacccgt
     2281 ctggatcgtt gggaaccgga actgaacgaa gcgattccga acgatgaacg tgataccacc
     2341 atgccggcag caatggcgac caccctgcgt aaactgctga cgggtgagct gctgaccctg
     2401 gcaagccgcc agcaactgat tgattggatg gaagcggata aagtggcggg tccgctgctg
     2461 cgtagcgcgc tgccggctgg ctggtttatt gcggataaaa gcggtgcggg cgaacgtggc
     2521 agccgtggca ttattgcggc gctgggcccg gatggtaaac cgagccgtat tgtggtgatt
     2581 tataccaccg gcagccaggc gacgatggat gaacgtaacc gtcagattgc ggaaattggc
     2641 gcgagcctga ttaaacattg gtaaaccgat acaattaaag gctccttttg gagccttttt
     2701 ttttggacga cccttgtcag atcaaaggat cttc
//
