LOCUS       sequence-464844-        2699 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Misc_Terminating-Modulator-A_PartType8-11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK124 [Misc_Terminating-Modulator-A_PartType...
FEATURES             Location/Qualifiers
     source          1..2699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     RBS             783..794
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             801..1478
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      1487..1558
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1574..1601
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        1677..1768
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1769..1837,1838..2629)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2633..2672
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctgctga acggtacgca ggtggaaagt gaaacgtgat ttcatgcgtc
      661 attttgaaca ttttgtaaat cttatttaat aatgtgtgcg gcaattcaca tttaatttat
      721 gaatgttttc ttaacatcgc ggcaactcaa gaaacggcag gttcggatct tagctactag
      781 agaaagagga gaaatactag atggcgagta gcgaagacgt tatcaaagag ttcatgcgtt
      841 tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg
      901 aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc
      961 tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta
     1021 aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg
     1081 aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc
     1141 aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc
     1201 cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag
     1261 acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg
     1321 acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca
     1381 aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt
     1441 acgaacgtgc tgaaggtcgt cactccaccg gtgcttaacc aggcatcaaa taaaacgaaa
     1501 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     1561 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatacac ctgcgtacgc
     1621 tggagttgag accagagtag cccgcctaat gagcgggctt ttttttaatt cccctatttg
     1681 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     1741 gcttcaataa tattgaaaaa ggaagagtat gagcattcag cattttcgtg tggcgctgat
     1801 tccgtttttt gcggcgtttt gcctgccggt gtttgcgcat ccggaaaccc tggtgaaagt
     1861 gaaagatgcg gaagatcaac tgggtgcgcg cgtgggctat attgaactgg atctgaacag
     1921 cggcaaaatt ctggaatctt ttcgtccgga agaacgtttt ccgatgatga gcacctttaa
     1981 agtgctgctg tgcggtgcgg ttctgagccg tgtggatgcg ggccaggaac aactgggccg
     2041 tcgtattcat tatagccaga acgatctggt ggaatatagc ccggtgaccg aaaaacatct
     2101 gaccgatggc atgaccgtgc gtgaactgtg cagcgcggcg attaccatga gcgataacac
     2161 cgcggcgaac ctgctgctga cgaccattgg cggtccgaaa gaactgaccg cgtttctgca
     2221 taacatgggc gatcatgtga cccgtctgga tcgttgggaa ccggaactga acgaagcgat
     2281 tccgaacgat gaacgtgata ccaccatgcc ggcagcaatg gcgaccaccc tgcgtaaact
     2341 gctgacgggt gagctgctga ccctggcaag ccgccagcaa ctgattgatt ggatggaagc
     2401 ggataaagtg gcgggtccgc tgctgcgtag cgcgctgccg gctggctggt ttattgcgga
     2461 taaaagcggt gcgggcgaac gtggcagccg tggcattatt gcggcgctgg gcccggatgg
     2521 taaaccgagc cgtattgtgg tgatttatac caccggcagc caggcgacga tggatgaacg
     2581 taaccgtcag attgcggaaa ttggcgcgag cctgattaaa cattggtaaa ccgatacaat
     2641 taaaggctcc ttttggagcc tttttttttg gacgaccctt gtcagatcaa aggatcttc
//
