LOCUS       sequence-464845-        2738 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Misc_Terminating-Modulator-B_PartType8-11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK125 [Misc_Terminating-Modulator-B_PartType...
FEATURES             Location/Qualifiers
     source          1..2738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     RBS             783..794
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             801..1514
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1526..1597
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1613..1640
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        1716..1807
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1808..1876,1877..2668)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2672..2711
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctgctga acggtacgca ggtggaaagt gaaacgtgat ttcatgcgtc
      661 attttgaaca ttttgtaaat cttatttaat aatgtgtgcg gcaattcaca tttaatttat
      721 gaatgttttc ttaacatcgc ggcaactcaa gaaacggcag gttcggatct tagctactag
      781 agaaagagga gaaatactag atgcgtaaag gcgaagagct gttcactggt gtcgtcccta
      841 ttctggtgga actggatggt gatgtcaacg gtcataagtt ttccgtgcgt ggcgagggtg
      901 aaggtgacgc aactaatggt aaactgacgc tgaagttcat ctgtactact ggtaaactgc
      961 cggttccttg gccgactctg gtaacgacgc tgacttatgg tgttcagtgc tttgctcgtt
     1021 atccggacca tatgaagcag catgacttct tcaagtccgc catgccggaa ggctatgtgc
     1081 aggaacgcac gatttccttt aaggatgacg gcacgtacaa aacgcgtgcg gaagtgaaat
     1141 ttgaaggcga taccctggta aaccgcattg agctgaaagg cattgacttt aaagaggacg
     1201 gcaatatcct gggccataag ctggaataca attttaacag ccacaatgtt tacatcaccg
     1261 ccgataaaca aaaaaatggc attaaagcga attttaaaat tcgccacaac gtggaggatg
     1321 gcagcgtgca gctggctgat cactaccagc aaaacactcc aatcggtgat ggtcctgttc
     1381 tgctgccaga caatcactat ctgagcacgc aaagcgttct gtctaaagat ccgaacgaga
     1441 aacgcgatca tatggttctg ctggagttcg taaccgcagc gggcatcacg catggtatgg
     1501 atgaactgta caaatgacca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1561 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     1621 ttcgggtggg cctttctgcg tttatacacc tgcgtacgct ggagttgaga ccagagtagc
     1681 ccgcctaatg agcgggcttt tttttaattc ccctatttgt ttatttttct aaatacattc
     1741 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     1801 gaagagtatg agcattcagc attttcgtgt ggcgctgatt ccgttttttg cggcgttttg
     1861 cctgccggtg tttgcgcatc cggaaaccct ggtgaaagtg aaagatgcgg aagatcaact
     1921 gggtgcgcgc gtgggctata ttgaactgga tctgaacagc ggcaaaattc tggaatcttt
     1981 tcgtccggaa gaacgttttc cgatgatgag cacctttaaa gtgctgctgt gcggtgcggt
     2041 tctgagccgt gtggatgcgg gccaggaaca actgggccgt cgtattcatt atagccagaa
     2101 cgatctggtg gaatatagcc cggtgaccga aaaacatctg accgatggca tgaccgtgcg
     2161 tgaactgtgc agcgcggcga ttaccatgag cgataacacc gcggcgaacc tgctgctgac
     2221 gaccattggc ggtccgaaag aactgaccgc gtttctgcat aacatgggcg atcatgtgac
     2281 ccgtctggat cgttgggaac cggaactgaa cgaagcgatt ccgaacgatg aacgtgatac
     2341 caccatgccg gcagcaatgg cgaccaccct gcgtaaactg ctgacgggtg agctgctgac
     2401 cctggcaagc cgccagcaac tgattgattg gatggaagcg gataaagtgg cgggtccgct
     2461 gctgcgtagc gcgctgccgg ctggctggtt tattgcggat aaaagcggtg cgggcgaacg
     2521 tggcagccgt ggcattattg cggcgctggg cccggatggt aaaccgagcc gtattgtggt
     2581 gatttatacc accggcagcc aggcgacgat ggatgaacgt aaccgtcaga ttgcggaaat
     2641 tggcgcgagc ctgattaaac attggtaaac cgatacaatt aaaggctcct tttggagcct
     2701 ttttttttgg acgacccttg tcagatcaaa ggatcttc
//
