LOCUS       sequence-464862-        1784 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Linker_MCS-L8_PartType4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK143 [Linker_MCS-L8_PartType4]
FEATURES             Location/Qualifiers
     source          1..1784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    628..684
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        762..853
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(854..922,923..1714)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1718..1757
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctcttggcg cggccgcaag cttgcatgcc tgcaggtcga ctctagagga
      661 tccccgggta ccgagctcga attcgcgcgg ccgcggccta ggcggccaac gtgagaccag
      721 agtagcccgc ctaatgagcg ggcttttttt taattcccct atttgtttat ttttctaaat
      781 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
      841 aaaaaggaag agtatgagca ttcagcattt tcgtgtggcg ctgattccgt tttttgcggc
      901 gttttgcctg ccggtgtttg cgcatccgga aaccctggtg aaagtgaaag atgcggaaga
      961 tcaactgggt gcgcgcgtgg gctatattga actggatctg aacagcggca aaattctgga
     1021 atcttttcgt ccggaagaac gttttccgat gatgagcacc tttaaagtgc tgctgtgcgg
     1081 tgcggttctg agccgtgtgg atgcgggcca ggaacaactg ggccgtcgta ttcattatag
     1141 ccagaacgat ctggtggaat atagcccggt gaccgaaaaa catctgaccg atggcatgac
     1201 cgtgcgtgaa ctgtgcagcg cggcgattac catgagcgat aacaccgcgg cgaacctgct
     1261 gctgacgacc attggcggtc cgaaagaact gaccgcgttt ctgcataaca tgggcgatca
     1321 tgtgacccgt ctggatcgtt gggaaccgga actgaacgaa gcgattccga acgatgaacg
     1381 tgataccacc atgccggcag caatggcgac caccctgcgt aaactgctga cgggtgagct
     1441 gctgaccctg gcaagccgcc agcaactgat tgattggatg gaagcggata aagtggcggg
     1501 tccgctgctg cgtagcgcgc tgccggctgg ctggtttatt gcggataaaa gcggtgcggg
     1561 cgaacgtggc agccgtggca ttattgcggc gctgggcccg gatggtaaac cgagccgtat
     1621 tgtggtgatt tataccaccg gcagccaggc gacgatggat gaacgtaacc gtcagattgc
     1681 ggaaattggc gcgagcctga ttaaacattg gtaaaccgat acaattaaag gctccttttg
     1741 gagccttttt ttttggacga cccttgtcag atcaaaggat cttc
//
