LOCUS       sequence-464869-        2706 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Empty Part Plasmid Vector, for cloning your 
            own parts into the system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK-Empty [Part Cloning Vector]
FEATURES             Location/Qualifiers
     source          1..2706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     RBS             766..777
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             784..1497
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1509..1580
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1596..1623
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        1684..1775
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1776..1844,1845..2636)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2640..2679
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg cgagacggaa agtgaaacgt gatttcatgc gtcattttga acattttgta
      661 aatcttattt aataatgtgt gcggcaattc acatttaatt tatgaatgtt ttcttaacat
      721 cgcggcaact caagaaacgg caggttcgga tcttagctac tagagaaaga ggagaaatac
      781 tagatgcgta aaggcgaaga gctgttcact ggtgtcgtcc ctattctggt ggaactggat
      841 ggtgatgtca acggtcataa gttttccgtg cgtggcgagg gtgaaggtga cgcaactaat
      901 ggtaaactga cgctgaagtt catctgtact actggtaaac tgccggttcc ttggccgact
      961 ctggtaacga cgctgactta tggtgttcag tgctttgctc gttatccgga ccatatgaag
     1021 cagcatgact tcttcaagtc cgccatgccg gaaggctatg tgcaggaacg cacgatttcc
     1081 tttaaggatg acggcacgta caaaacgcgt gcggaagtga aatttgaagg cgataccctg
     1141 gtaaaccgca ttgagctgaa aggcattgac tttaaagagg acggcaatat cctgggccat
     1201 aagctggaat acaattttaa cagccacaat gtttacatca ccgccgataa acaaaaaaat
     1261 ggcattaaag cgaattttaa aattcgccac aacgtggagg atggcagcgt gcagctggct
     1321 gatcactacc agcaaaacac tccaatcggt gatggtcctg ttctgctgcc agacaatcac
     1381 tatctgagca cgcaaagcgt tctgtctaaa gatccgaacg agaaacgcga tcatatggtt
     1441 ctgctggagt tcgtaaccgc agcgggcatc acgcatggta tggatgaact gtacaaatga
     1501 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1561 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1621 gcgtttatac gtctctgacc agagtagccc gcctaatgag cgggcttttt tttaattccc
     1681 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     1741 gataaatgct tcaataatat tgaaaaagga agagtatgag cattcagcat tttcgtgtgg
     1801 cgctgattcc gttttttgcg gcgttttgcc tgccggtgtt tgcgcatccg gaaaccctgg
     1861 tgaaagtgaa agatgcggaa gatcaactgg gtgcgcgcgt gggctatatt gaactggatc
     1921 tgaacagcgg caaaattctg gaatcttttc gtccggaaga acgttttccg atgatgagca
     1981 cctttaaagt gctgctgtgc ggtgcggttc tgagccgtgt ggatgcgggc caggaacaac
     2041 tgggccgtcg tattcattat agccagaacg atctggtgga atatagcccg gtgaccgaaa
     2101 aacatctgac cgatggcatg accgtgcgtg aactgtgcag cgcggcgatt accatgagcg
     2161 ataacaccgc ggcgaacctg ctgctgacga ccattggcgg tccgaaagaa ctgaccgcgt
     2221 ttctgcataa catgggcgat catgtgaccc gtctggatcg ttgggaaccg gaactgaacg
     2281 aagcgattcc gaacgatgaa cgtgatacca ccatgccggc agcaatggcg accaccctgc
     2341 gtaaactgct gacgggtgag ctgctgaccc tggcaagccg ccagcaactg attgattgga
     2401 tggaagcgga taaagtggcg ggtccgctgc tgcgtagcgc gctgccggct ggctggttta
     2461 ttgcggataa aagcggtgcg ggcgaacgtg gcagccgtgg cattattgcg gcgctgggcc
     2521 cggatggtaa accgagccgt attgtggtga tttataccac cggcagccag gcgacgatgg
     2581 atgaacgtaa ccgtcagatt gcggaaattg gcgcgagcct gattaaacat tggtaaaccg
     2641 atacaattaa aggctccttt tggagccttt ttttttggac gacccttgtc agatcaaagg
     2701 atcttc
//
