LOCUS       sequence-46488-e         688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Alford SC, Abdelfattah AS, Ding Y, Campbell RE
  TITLE     A fluorogenic red fluorescent protein heterodimer.
  JOURNAL   Chem Biol. 2012 Mar 23;19(3):353-60.
  PUBMED    22444590
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem 
            Biol."; date: "2012-03-23"; volume: "19(3)"; pages: "353-60"
COMMENT     Sequence Label: pDDRFPA1-CaM
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(239..257)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     507..526
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcgtttaaa cttaagcttg ccaccatggt gagcaagagc
      121 gaggaggtca tcaaagagtt catgcgcttc aaggtgcgct tggagggctc catgaacggc
      181 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      241 aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag
      301 atcatgtacg gctccaaggc gtacgtgaag caccccgccg acgtccccga ttacatgaag
      361 ctgtccttcc ccgagggctt caagtgggag cgcgtgatgc acttcgagga cggcggtctg
      421 gtgaccgtga ctcaggacac ctccctgcag gacggcacgc tgatctacaa ggtgaagatg
      481 cgcggcacca acttcccccc cgacggcccc gtaatgcaga agaagacctt gggctgggat
      541 tatgccaccg agcgcctgta ccccgaagac ggcgtgctga agggcgagct tctggggcgc
      601 ctgaagctga aggacggcgg cctcaacctg gtggagttca agaccatcta catggccaag
      661 aagcccgtgc aactgcccgg ctactact
//
