LOCUS       sequence-464903-        8486 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a fusion between the Murine Leukemia Virus Gag polyprotein
            and the human DDX3 protein to produce virus-like particles loaded 
            with DDX3 and deliver it to target cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8486)
  AUTHORS   Labaronne E, Decimo D, Bertrand L, Guiguettaz L, Sohier TJM, Cluet 
            D, Vivet-Boudou V, Chaves Valadao AL, Dahoui C, Francois P, Hatin I,
            Lambotte O, Samri A, Autran B, Etienne L, Goujon C, Paillart JC, 
            Namy O, Ramirez BC, Ohlmann T, Moris A, Ricci EP
  TITLE     Non-AUG HIV-1 uORF translation elicits specific T cell immune 
            response and regulates viral transcript expression.
  JOURNAL   Nat Commun. 2025 Feb 18;16(1):1706. doi: 10.1038/s41467-025-56772-3.
  PUBMED    39966383
REFERENCE   2  (bases 1 to 8486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-02-18"; volume: "16(1):1706. doi"; pages: " 
            10.1038/s41467-025-56772-3"
COMMENT     Sequence Label: BIC-Gag-DDX3
FEATURES             Location/Qualifiers
     source          1..8486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1639..1662
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(3733..3752)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3798..3853
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3852..3871)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4211..4227)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4211..4227)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4224..4246)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4235..4251
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4259..4265,4266..4283,4284..4289))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4304..4325
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4442..4459)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4613..5201)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4693..4712)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5372..6163,6164..6232))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5995..6014
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6233..6337)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6363..6818)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6500..6521)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6712..6731
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6945..6967
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6959..6976
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6960..6976
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6986..7005
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6986..7004
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        7125..7504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7505..7708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7659..7679
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7705..7729
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          7820..8392
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
ORIGIN
        1 atgggccagg ctgttaccac ccccttaagt ttgactttag accactggaa ggatgtcgaa
       61 cggacagccc acaacctgtc ggtagaggtt agaaaaaggc gctgggttac attctgctct
      121 gcagaatggc caaccttcaa cgtcggatgg ccacgagacg gcacttttaa cccagacatt
      181 attacacagg ttaagatcaa ggtcttctca cctggcccac atggacatcc ggatcaggtc
      241 ccctacatcg tgacctggga agctatagca gtagaccccc ctccctgggt cagacccttc
      301 gtgcacccta aacctcccct ctctcttccc ccttcagccc cctctctccc acctgaaccc
      361 ccactctcga ccccgcccca gtcctccctc tatccggctc tcacttctcc tttaaacacc
      421 aaacctaggc ctcaagtcct tcctgatagc ggaggaccac tcattgatct actcacggag
      481 gaccctccgc cttaccggga cccagggcca ccctctcctg acgggaacgg cgatagcgga
      541 gaagtggccc ctacagaagg agcccctgac ccttccccaa tggtatcccg cctgcgggga
      601 agaaaagaac cccccgtggc ggattctact acctctcagg cgttccccct tcgcctggga
      661 gggaatggac agtatcaata ctggccattt tcctcctctg acctctataa ctggaaaaat
      721 aacaacccct ctttctccga ggacccagct aaattgacag ctttgatcga gtccgttctc
      781 cttactcatc agcccacttg ggatgactgc caacagctat tagggaccct gctgacggga
      841 gaagaaaaac agcgagtgct cctagaggcc cgaaaggcgg ttcgagggga ggacggacgc
      901 ccaactcagc tgcccaatga cattaatgat gcttttccct tggaacgtcc cgactgggac
      961 tacaacaccc aacgaggtag gaaccaccta gtccactatc gccagttgct cctagcgggt
     1021 ctccaaaacg cgggcagaag ccccaccaat ttggccaagg taaaagggat aacccaggga
     1081 cctaatgagt ctccctcagc ctttttagag agactcaagg aggcctatcg cagatacact
     1141 ccttatgacc ctgaggaccc agggcaagaa accaatgtgg ccatgtcatt catctggcag
     1201 tccgccccgg atatcgggcg aaagttagag cggttagaag atttgaagag taagacctta
     1261 ggagacttag tgagggaagc tgaaaagatc tttaataaac gagaaacccc ggaagaaaga
     1321 gaggaacgta ttaggagaga aacagaggaa aaggaagaac gccgtagggc agaggatgtg
     1381 cagagagaga aggagaggga ccgcagaaga catagagaaa tgagtaagtt gctggctact
     1441 gtcgttagcg ggcagagaca ggatagacag ggaggagagc gaaggaggcc ccaactcgac
     1501 cacgaccagt gtgcctactg caaagaaaag ggacattggg ctagagattg ccccaagaag
     1561 ccaagaggac cccggggacc acgaccccag gcctccctcc tgacgcgtag ttccctgtat
     1621 ccagccctca cacctaccga ctacaaagac gatgacgaca agagtcatgt ggcagtggaa
     1681 aatgcgctcg ggctggacca gcagtttgct ggcctagacc tgaactcttc agataatcag
     1741 agtggaggaa gtacagccag caaagggcgc tatattcctc ctcatttaag gaaccgagaa
     1801 gctactaaag gtttctacga taaagacagt tcagggtgga gttctagcaa agataaggat
     1861 gcgtatagca gttttggatc tcgtagtgat tcaagaggga agtctagctt cttcagtgat
     1921 cgtggaagtg gatcaagggg aaggtttgat gatcgtggac ggagtgatta cgatggcatt
     1981 ggcagccgtg gtgacagaag tggctttggc aaatttgaac gtggtggaaa cagtcgctgg
     2041 tgtgacaaat cagatgaaga tgattggtca aaaccactcc caccaagtga acgcttggaa
     2101 caggaactct tttctggagg caacactggg attaattttg agaaatacga tgacattcca
     2161 gttgaggcaa caggcaacaa ctgtcctcca catattgaaa gtttcagtga tgttgagatg
     2221 ggagaaatta tcatgggaaa cattgagctt actcgttata ctcgcccaac tccagtgcaa
     2281 aagcatgcta ttcctattat caaagagaaa agagacttga tggcttgtgc ccaaacaggg
     2341 tctggaaaaa ctgcagcatt tctgttgccc atcttgagtc agatttattc agatggtcca
     2401 ggcgaggctt tgagggccat gaaggaaaat ggaaggtatg ggcgccgcaa acaataccca
     2461 atctccttgg tattagcacc aacgagagag ttggcagtac agatctacga ggaagccaga
     2521 aaattttcat accgatctag agttcgtcct tgcgtggttt atggtggtgc cgatattggt
     2581 cagcagattc gagacttgga acgtggatgc catttgttag tagccactcc aggacgtcta
     2641 gtggatatga tggaaagagg aaagattgga ttagactttt gcaaatactt ggtgttagat
     2701 gaagctgatc ggatgttgga tatggggttt gagcctcaga ttcgtagaat agtcgaacaa
     2761 gatactatgc ctccaaaggg tgtccgccac actatgatgt ttagtgctac ttttcctaag
     2821 gaaatacaga tgctggctcg tgatttctta gatgaatata tcttcttggc tgtaggaaga
     2881 gttggctcta cctctgaaaa catcacacag aaagtagttt gggtggaaga atcagacaaa
     2941 cggtcatttc tgcttgacct cctaaatgca acaggcaagg attcactgac cttagtgttt
     3001 gtggagacca aaaagggtgc agattctctg gaggatttct tataccatga aggatacgca
     3061 tgtaccagca tccatggaga ccgttctcag agggatagag aagaggccct tcaccagttc
     3121 cgctcaggaa aaagcccaat tttagtggct acagcagtag cagcaagagg actggacatt
     3181 tcaaatgtga aacatgttat caattttgac ttgccaagtg atattgaaga atatgtacat
     3241 cgtattggtc gtacgggacg tgtaggaaac cttggcctgg caacctcatt ctttaacgag
     3301 aggaacataa atattactaa ggatttgttg gatcttcttg ttgaagctaa acaagaagtg
     3361 ccgtcttggt tagaaaacat ggcttatgaa caccactaca agggtagcag tcgtggacgt
     3421 tctaagagta gcagatttag tggagggttt ggtgccagag actaccgaca aagtagcggt
     3481 gccagcagtt ccagcttcag cagcagccgc gcaagcagca gccgcagtgg cggaggtggc
     3541 cacggtagca gcagaggatt tggtggaggt ggctatggag gcttttacaa cagtgatgga
     3601 tatggaggaa attataactc ccagggggtt gactggtggg gtaactaagt acctgcatcc
     3661 tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa
     3721 ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga
     3781 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     3841 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga
     3901 gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg
     3961 gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag
     4021 aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt
     4081 aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact
     4141 actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacggat cggccgcaat
     4201 tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     4261 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     4321 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     4381 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     4441 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     4501 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     4561 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     4621 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     4681 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     4741 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     4801 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     4861 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     4921 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     4981 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     5041 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     5101 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     5161 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     5221 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     5281 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     5341 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     5401 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     5461 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     5521 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     5581 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     5641 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     5701 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     5761 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     5821 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     5881 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     5941 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     6001 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     6061 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     6121 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     6181 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     6241 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     6301 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     6361 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     6421 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     6481 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     6541 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     6601 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     6661 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     6721 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     6781 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
     6841 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     6901 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     6961 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
     7021 ccgcggtggc ggccgctcta gagagcttgg cccattgcat acgttgtatc catatcataa
     7081 tatgtacatt tatattggct catgtccaac attaccgcca tgttgacatt gattattgac
     7141 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     7201 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     7261 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     7321 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     7381 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     7441 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     7501 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     7561 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     7621 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     7681 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
     7741 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggtcgac
     7801 cgatcctgag aacttcaggg tgagtttggg gacccttgat tgttctttct ttttcgctat
     7861 tgtaaaattc atgttatatg gagggggcaa agttttcagg gtgttgttta gaatgggaag
     7921 atgtcccttg tatcaccatg gaccctcatg ataattttgt ttctttcact ttctactctg
     7981 ttgacaacca ttgtctcctc ttattttctt ttcattttct gtaacttttt cgttaaactt
     8041 tagcttgcat ttgtaacgaa tttttaaatt cacttttgtt tatttgtcag attgtaagta
     8101 ctttctctaa tcactttttt ttcaaggcaa tcagggtata ttatattgta cttcagcaca
     8161 gttttagaga acaattgtta taattaaatg ataaggtaga atatttctgc atataaattc
     8221 tggctggcgt ggaaatattc ttattggtag aaacaactac accctggtca tcatcctgcc
     8281 tttctcttta tggttacaat gatatacact gtttgagatg aggataaaat actctgagtc
     8341 caaaccgggc ccctctgcta accatgttca tgccttcttc tctttcctac agctcctggg
     8401 caacgtgctg gttgttgtgc tgtctcatca ttttggcaaa gaattcctcg agggccggat
     8461 ccgaattcct gtatttgtct gaaaac
//
