LOCUS       sequence-46491-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Alford SC, Abdelfattah AS, Ding Y, Campbell RE
  TITLE     A fluorogenic red fluorescent protein heterodimer.
  JOURNAL   Chem Biol. 2012 Mar 23;19(3):353-60.
  PUBMED    22444590
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem 
            Biol."; date: "2012-03-23"; volume: "19(3)"; pages: "353-60"
COMMENT     Sequence Label: pM13-DDRFPB1
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(128..151)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(326..345)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     595..613
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 cctctagact cgagttagct cccgatatca agacctgcta atttcaaggc taacttgtac
       61 cgctcgtcca tgcccagacg gtggcggccc tcggagcgcc cgtactgttc cacgatggtg
      121 tagtcctcgt tgtgggaggt gatgtccagc ttggtgtcca cacagtaatc gccgggcagt
      181 tgcacgggct tcttggccat gtagatggtc tcgaactcca ccaggtagtg gccgccgtcc
      241 ttcagcttca gggcctgata gctatggccc ttcagcacgc cgtcttcggg gtacagcatc
      301 tcggtggagg cctcccagcc catggtcttc ttctgcatta cggggccgtc gggggggaag
      361 ttggtgccgc gcatcttcac cttgcagatc agcgtgccgt cctgcaggga ggagtcctgg
      421 gtaacggtca ccagaccgcc gtcttcgaag ttcatcacgc gctcccactt gaagccctcg
      481 gggaagggca gcttcttgta atcggggatg tcggcggggt gcctcacgta cgcctcggag
      541 ccgtacatga actgggggga caggatgtcc caggcgaagg gcagggggcc gcccttggtc
      601 accttcagct tggcggtctg ggtgccctcg taggggcggc cctcgccctc gccctcgatc
      661 tcgaactcgt ggccgttcat ggagccctcc atgcgcacct tgaagcgcat gaactctttg
      721 atggtggcca tgttgttgtc ctcggaggag gcggtaccgg agctgccgct gccggtgctg
      781 ccggtgccat gccccagtgc cccggagctg gagatcttct tcagtcttcc gaatgctcgg
      841 acagcatggc ctgt
//
