LOCUS       sequence-464911-       12211 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Stable expression of eGFP-firefly luciferase (ffLuc).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12211)
  AUTHORS   Ely ZA, Kulstad ZJ, Gunaydin G, Addepalli S, Verzani EK, Casarrubios
            M, Clauser KR, Wang X, Lippincott IE, Louvet C, Schmitt T, Kapner 
            KS, Agus MP, Hennessey CJ, Cleary JM, Hadrup SR, Klaeger S, Su J, 
            Jaeger AM, Wolpin BM, Raghavan S, Smith EL, Greenberg PD, Aguirre 
            AJ, Abelin JG, Carr SA, Jacks T, Freed-Pastor WA.
  TITLE     Pancreatic cancer-restricted cryptic antigens are targets for T cell
            recognition.
  JOURNAL   Science. 2025. May 8; 388(6747)
REFERENCE   2  (bases 1 to 12211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2025. May 8; 388(6747)"
COMMENT     Sequence Label: pWF073_LVt-EF1a-eGFP-ffLuc-PGK-BlastR
FEATURES             Location/Qualifiers
     source          1..12211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2638..3816
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2869..3807
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3764..3784
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3847..3856
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3853..3855,3856..3858,3859..4569)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3853..3855,3856..3858,3859..4569)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3898..3919)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4159..4178)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4506..4527
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        6291..6790
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6326..6349)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6723..6742
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6815..7213
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    7303..7891
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7356..7376)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7774..7785)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7963..8196
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8222..8239)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8228..8452
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8498..8926
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8585..8604)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8795..8816
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8935..8955)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8940..9269
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9120..9255
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9182..9201
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9317..9364
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9383..9757
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9887..10008
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9924..9943)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9978..9997
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10057..10073)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10057..10073)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10070..10092)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10081..10097
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10105..10111,10112..10129,10130..10135))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10150..10171
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10288..10305)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10459..11047)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10539..10558)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11218..12009,12010..12078))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11841..11860
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12079..12183)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aggtaccgat cagtgtgagg gagtgtaaag ctggtttggc
     2641 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
     2701 ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
     2761 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
     2821 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg
     2881 tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact
     2941 tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag
     3001 agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct
     3061 gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc
     3121 gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa
     3181 gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg
     3241 ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg
     3301 cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc
     3361 ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt
     3421 gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg
     3481 cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc
     3541 tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag
     3601 ttctagagct tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag
     3661 tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc
     3721 tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg
     3781 gttcaaagtt tttttcttcc atttcaggtg tcgtgactaa ctcgaacgct agctgtgcga
     3841 tcgtttgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     3901 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     3961 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     4021 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     4081 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     4141 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     4201 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     4261 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     4321 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     4381 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     4441 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     4501 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     4561 ctgtacaagt ccggccggac tcagatttcg agctcaagct tcgaattcga aaccgaacgc
     4621 gaagagaatg tcgtttatgg gcccttgccc ttttatccaa tcgaagaagg aagtgcgggc
     4681 attcaactgc acaagtatat gcaacaatat gctaagcttg gcgctattgc tttttccaat
     4741 gcgcttaccg gggttgacat ttcatatcaa caatatttcg atattacgtg taggctcgca
     4801 gaagcaatga aaaattatgg tatgaaacct gaagggcaca tagctttgtg ttctgaaaat
     4861 tgcgaggagt tctttatacc cgtccttgcc gggctttaca ttggcgtgac tgtggccccc
     4921 acaaacgaaa tatacaccct cagggagctt aatcactcat tgggaattgc acagcccacg
     4981 attgtttttt cctcacgcaa gggtcttccc aaagtactcg aagtccagaa aacagtaact
     5041 tgcataaaaa cgattgtcat ccttgactct aaagttaatt tcgggggtta cgattgcgtt
     5101 gaaacattta tcaagaagca tgtggagctt ggtttccccg caacttcatt tgttccaatt
     5161 gacgttaagg accgcaagca tcacatagct ctgctgatga actcaagcgg ctcaacggga
     5221 ctccccaagg gggttgagat tacacatgaa ggcactgtaa ctagattcag tcacgcaaaa
     5281 gacccaatct atggtaatca ggtatcacct gggacggcga ttcttacagt ggtaccattc
     5341 catcacgggt tcggaatgtt cactacattg ggctatttcg cgtgtgggta tcgcatagtt
     5401 atgttgacta agttcgacga agagttgttt ctgcgaacgc tgcaggacta caagtgcact
     5461 tcagtcattc tggtcccgac gctgttcgct attctcaaca gatccgaatt gctcgataag
     5521 ttcgatcttt caaacctgac ggaaattgcg tcaggtggcg ccccgcttgc caaagaaatc
     5581 ggagaagccg tcgctcgccg ctttaatctc ccaggggtga ggcagggcta cggtcttacc
     5641 gagacgactt cagcattcat cataacccct gaaggcgatg ataaaccagg ggcatccggg
     5701 aaggtggtac ccctgttcaa agtaaagata attgacttgg ataccaagaa gacacttggt
     5761 gtcaatcgcc ggggtgagat atgcgtcaaa ggaccgtctc tgatgcttgg ctacacaaat
     5821 aatcctgaag ctaccaggga gaccatagat gaggagggtt ggctgcatac tggagacata
     5881 ggctattatg atgaagacga acactttttc atcgtagaca ggctgaagag tctcatcaag
     5941 tacaaaggat atcaggtgcc ccccgccgag ctcgaaagcg ttctcctgca acacccaaat
     6001 atttttgacg caggagtcgc cggagtgccc gatagcgagg cgggggagct gccaggagct
     6061 gtcgttgtca tggaaaaagg caagacgatg acagaaaaag aaatagtgga ctatgtcaat
     6121 agccaagtag taaatcacaa gaggcttaga ggtggcgtaa ggttcgtaga tgaagtacca
     6181 aaagggttga cgggtaaaat tgacgcgaaa gtaatcaggg agattttgaa aaagccgcaa
     6241 gctaagatgt aaaaaccgct gttcctagga atcccgaggc ctattctacc gggtagggga
     6301 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg
     6361 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc
     6421 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
     6481 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
     6541 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg
     6601 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg
     6661 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc
     6721 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc
     6781 gggagacccg acattagcgc tacagcttaa gcggatggcc aagcctttgt ctcaagaaga
     6841 atccaccctc attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta
     6901 cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata
     6961 tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc
     7021 agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc
     7081 ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa
     7141 ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt
     7201 gtgggagggc taaatcgaaa cgttgttgtt tggggttgaa ttactctaca tgtttaaggg
     7261 ttccggttcc actaggtaca attcgatatc aagcttatcg ataatcaacc tctggattac
     7321 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     7381 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     7441 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     7501 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     7561 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     7621 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     7681 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     7741 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     7801 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     7861 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     7921 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     7981 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     8041 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     8101 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     8161 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccggcgaaac ccgctgatca
     8221 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     8281 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     8341 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     8401 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     8461 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     8521 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     8581 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     8641 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     8701 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     8761 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     8821 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     8881 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     8941 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     9001 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     9061 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     9121 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     9181 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     9241 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     9301 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     9361 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
     9421 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
     9481 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
     9541 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
     9601 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
     9661 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
     9721 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
     9781 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     9841 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     9901 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     9961 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
    10021 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
    10081 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
    10141 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
    10201 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
    10261 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
    10321 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
    10381 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
    10441 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    10501 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    10561 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    10621 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    10681 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    10741 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    10801 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    10861 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
    10921 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    10981 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    11041 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    11101 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    11161 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    11221 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    11281 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    11341 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    11401 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    11461 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    11521 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    11581 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    11641 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    11701 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    11761 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    11821 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    11881 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    11941 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    12001 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    12061 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    12121 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    12181 gcgcacattt ccccgaaaag tgccacctga c
//
