LOCUS       sequence-464912-       10664 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ncHLAp-reactive T cell receptor (TCR012H).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10664)
  AUTHORS   Ely ZA, Kulstad ZJ, Gunaydin G, Addepalli S, Verzani EK, Casarrubios
            M, Clauser KR, Wang X, Lippincott IE, Louvet C, Schmitt T, Kapner 
            KS, Agus MP, Hennessey CJ, Cleary JM, Hadrup SR, Klaeger S, Su J, 
            Jaeger AM, Wolpin BM, Raghavan S, Smith EL, Greenberg PD, Aguirre 
            AJ, Abelin JG, Carr SA, Jacks T, Freed-Pastor WA.
  TITLE     Pancreatic cancer-restricted cryptic antigens are targets for T cell
            recognition.
  JOURNAL   Science. 2025. May 8; 388(6747)
REFERENCE   2  (bases 1 to 10664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2025. May 8; 388(6747)"
COMMENT     Sequence Label: TCR012H
FEATURES             Location/Qualifiers
     source          1..10664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2638..3816
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2869..3807
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3764..3784
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4792..4848
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    5756..6344
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5809..5829)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6227..6238)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6416..6649
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6675..6692)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6681..6905
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6951..7379
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7038..7057)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7248..7269
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7388..7408)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7393..7722
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7573..7708
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7635..7654
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7770..7817
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7836..8210
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8340..8461
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8377..8396)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8431..8450
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8510..8526)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8510..8526)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8523..8545)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8534..8550
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8558..8564,8565..8582,8583..8588))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8603..8624
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8741..8758)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8912..9500)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8992..9011)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9671..10462,10463..10531))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10294..10313
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10532..10636)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aggtaccgat cagtgtgagg gagtgtaaag ctggtttggc
     2641 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
     2701 ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
     2761 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
     2821 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg
     2881 tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact
     2941 tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag
     3001 agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct
     3061 gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc
     3121 gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa
     3181 gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg
     3241 ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg
     3301 cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc
     3361 ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt
     3421 gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg
     3481 cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc
     3541 tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag
     3601 ttctagagct tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag
     3661 tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc
     3721 tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg
     3781 gttcaaagtt tttttcttcc atttcaggtg tcgtgactaa ctcgaacgct agctgtgcga
     3841 tcgtttgcca ccatgctctg ctctctcctt gcccttctcc tgggcacttt ctttggggtc
     3901 agatctcaga ctattcatca atggccagcg accctggtgc agcctgtggg cagcccgctc
     3961 tctctggagt gcactgtgga gggaacatca aaccccaacc tatactggta ccgacaggct
     4021 gcaggcaggg gcctccagct gctcttctac tccgttggta ttggccagat cagctctgag
     4081 gtgccccaga atctctcagc ctccagaccc caggaccggc agttcatcct gagttctaag
     4141 aagctccttc tcagtgactc tggcttctat ctctgtgcct ggagtgtaac aggggactat
     4201 ggctacacct tcggttcggg gaccaggtta accgttgtag aggacctgaa caaggtgttc
     4261 ccacccgagg tcgctgtgtt tgagccatca gaagcagaga tctcccacac ccaaaaggcc
     4321 acactggtgt gcctggccac aggcttcttc cctgaccacg tggagctgag ctggtgggtg
     4381 aatggcaaag aagtgcattc tggggtctgc acggacccgc agcccctcaa ggagcagccc
     4441 gccctcaatg actccagata ctgcctgagc agccgcctga gggtctcggc caccttctgg
     4501 cagaaccccc gcaaccactt ccgctgtcaa gtccagttct acgggctctc ggagaatgac
     4561 gagtggaccc aggatagggc caaacccgtc acccagatcg tcagcgccga ggcctggggt
     4621 agagcagact gtggctttac ctcggtgtcc taccagcaag gggtcctgtc tgccaccatc
     4681 ctctatgaga tcctgctagg gaaggccacc ctgtatgctg tgctggtcag cgcccttgtg
     4741 ttgatggcca tggtcaagag aaaggatttc agagccaaaa gaggaagcgg cgccacaaac
     4801 ttctcactgc tgaaacaggc cggcgacgtg gaagagaatc ccggacctat ggcatgccct
     4861 ggcttcctgt gggcacttgt gatctccacc tgtcttgaat ttagcatggc tcagacagtc
     4921 actcagtctc aaccagagat gtctgtgcag gaggcagaga ccgtgaccct gagctgcaca
     4981 tatgacacca gtgagagtga ttattattta ttctggtaca agcagcctcc cagcaggcag
     5041 atgattctcg ttattcgcca agaagcttat aagcaacaga atgcaacaga gaatcgtttc
     5101 tctgtgaact tccagaaagc agccaaatcc ttcagtctca agatctcaga ctcacagctg
     5161 ggggatgccg cgatgtattt ctgtgtagaa aattccgggt atgcactcaa cttcggcaaa
     5221 ggcacctcgc tgttggtcac accccatatc cagaaccctg accctgctgt gtatcagctg
     5281 cgtgatagca aatccagtga caagtctgtc tgcctattca ccgattttga ttctcaaaca
     5341 aatgtgtcac aaagtaagga ttctgatgtg tatatcacag acaaatgcgt gctagacatg
     5401 aggtctatgg acttcaagag caacagtgct gtggcctgga gcaacaaatc tgactttgca
     5461 tgtgcaaacg ccttcaacaa cagcattatt ccagaagaca ccttcttccc cagcccagaa
     5521 agttcctgtg atgtcaagct ggtcgagaaa agctttgaaa cagatacgaa cctaaacttt
     5581 caaaacctgt cagtgattgg gttccgaatc ctcctcctga aagtggccgg gtttaatctg
     5641 ctcatgacgc tgcggctgtg gtccagctga aaacgttgtt gtttggggtt gaattactct
     5701 acatgtttaa gggttccggt tccactaggt acaattcgat atcaagctta tcgataatca
     5761 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     5821 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     5881 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     5941 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     6001 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     6061 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     6121 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     6181 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     6241 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     6301 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca
     6361 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     6421 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     6481 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     6541 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     6601 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccggcga
     6661 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     6721 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     6781 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     6841 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     6901 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     6961 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     7021 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     7081 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     7141 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     7201 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     7261 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     7321 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     7381 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     7441 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     7501 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     7561 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     7621 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     7681 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     7741 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg
     7801 gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc
     7861 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
     7921 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
     7981 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
     8041 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
     8101 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
     8161 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac
     8221 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     8281 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     8341 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     8401 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     8461 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     8521 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     8581 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     8641 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     8701 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     8761 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     8821 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     8881 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     8941 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     9001 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     9061 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     9121 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     9181 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     9241 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     9301 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     9361 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     9421 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     9481 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     9541 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     9601 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     9661 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     9721 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     9781 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     9841 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     9901 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     9961 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    10021 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    10081 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    10141 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    10201 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    10261 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    10321 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    10381 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    10441 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    10501 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    10561 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    10621 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac
//
