LOCUS       sequence-464913-       10670 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ncHLAp-reactive T cell receptor (TCR001H).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10670)
  AUTHORS   Ely ZA, Kulstad ZJ, Gunaydin G, Addepalli S, Verzani EK, Casarrubios
            M, Clauser KR, Wang X, Lippincott IE, Louvet C, Schmitt T, Kapner 
            KS, Agus MP, Hennessey CJ, Cleary JM, Hadrup SR, Klaeger S, Su J, 
            Jaeger AM, Wolpin BM, Raghavan S, Smith EL, Greenberg PD, Aguirre 
            AJ, Abelin JG, Carr SA, Jacks T, Freed-Pastor WA.
  TITLE     Pancreatic cancer-restricted cryptic antigens are targets for T cell
            recognition.
  JOURNAL   Science. 2025. May 8; 388(6747)
REFERENCE   2  (bases 1 to 10670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2025. May 8; 388(6747)"
COMMENT     Sequence Label: TCR001H
FEATURES             Location/Qualifiers
     source          1..10670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2638..3816
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2869..3807
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3764..3784
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3847..3856
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4801..4857
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    5762..6350
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5815..5835)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6233..6244)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6422..6655
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6681..6698)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6687..6911
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6957..7385
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7044..7063)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7254..7275
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7394..7414)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7399..7728
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7579..7714
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7641..7660
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7776..7823
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7842..8216
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8346..8467
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8383..8402)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8437..8456
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8516..8532)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8516..8532)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8529..8551)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8540..8556
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8564..8570,8571..8588,8589..8594))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8609..8630
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8747..8764)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8918..9506)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8998..9017)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9677..10468,10469..10537))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10300..10319
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10538..10642)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aggtaccgat cagtgtgagg gagtgtaaag ctggtttggc
     2641 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
     2701 ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
     2761 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
     2821 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg
     2881 tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact
     2941 tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag
     3001 agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct
     3061 gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc
     3121 gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa
     3181 gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg
     3241 ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg
     3301 cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc
     3361 ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt
     3421 gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg
     3481 cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc
     3541 tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag
     3601 ttctagagct tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag
     3661 tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc
     3721 tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg
     3781 gttcaaagtt tttttcttcc atttcaggtg tcgtgactaa ctcgaacgct agctgtgcga
     3841 tcgtttgcca ccatgggaat caggctcctc tgtcgtgtgg ccttttgttt cctggctgta
     3901 ggcctcgtag atgtgaaagt aacccagagc tcgagatatc tagtcaaaag gacgggagag
     3961 aaagtttttc tggaatgtgt ccaggatatg gaccatgaaa atatgttctg gtatcgacaa
     4021 gacccaggtc tggggctacg gctgatctat ttctcatatg atgttaaaat gaaagaaaaa
     4081 ggagatattc ctgaggggta cagtgtctct agagagaaga aggagcgctt ctccctgatt
     4141 ctggagtccg ccagcaccaa ccagacatct atgtacctct gtgccagcag tttaccagga
     4201 agcaatcagc cccagcattt tggtgatggg actcgactct ccatcctaga ggacctgaac
     4261 aaggtgttcc cacccgaggt cgctgtgttt gagccatcag aagcagagat ctcccacacc
     4321 caaaaggcca cactggtgtg cctggccaca ggcttcttcc ctgaccacgt ggagctgagc
     4381 tggtgggtga atggcaaaga agtgcattct ggggtctgca cggacccgca gcccctcaag
     4441 gagcagcccg ccctcaatga ctccagatac tgcctgagca gccgcctgag ggtctcggcc
     4501 accttctggc agaacccccg caaccacttc cgctgtcaag tccagttcta cgggctctcg
     4561 gagaatgacg agtggaccca ggatagggcc aaacccgtca cccagatcgt cagcgccgag
     4621 gcctggggta gagcagactg tggctttacc tcggtgtcct accagcaagg ggtcctgtct
     4681 gccaccatcc tctatgagat cctgctaggg aaggccaccc tgtatgctgt gctggtcagc
     4741 gcccttgtgt tgatggccat ggtcaagaga aaggatttca gagccaaaag aggaagcggc
     4801 gccacaaact tctcactgct gaaacaggcc ggcgacgtgg aagagaatcc cggacctatg
     4861 gagaccctct tgggcctgct tatcctttgg ctgcagctgc aatgggtgag cagcaaacag
     4921 gaggtgacac agattcctgc agctctgagt gtcccagaag gagaaaactt ggttctcaac
     4981 tgcagtttca ctgatagcgc tatttacaac ctccagtggt ttaggcagga ccctgggaaa
     5041 ggtctcacat ctctgttgct tattcagtca agtcagagag agcaaacaag tggaagactt
     5101 aatgcctcgc tggataaatc atcaggacgt agtactttat acattgcagc ttctcagcct
     5161 ggtgactcag ccacctacct ctgtgctgtg cttggggata gcaactatca gttaatctgg
     5221 ggcgctggga ccaagctaat tataaagcca gatatccaga accctgaccc tgctgtgtat
     5281 cagctgcgtg atagcaaatc cagtgacaag tctgtctgcc tattcaccga ttttgattct
     5341 caaacaaatg tgtcacaaag taaggattct gatgtgtata tcacagacaa atgcgtgcta
     5401 gacatgaggt ctatggactt caagagcaac agtgctgtgg cctggagcaa caaatctgac
     5461 tttgcatgtg caaacgcctt caacaacagc attattccag aagacacctt cttccccagc
     5521 ccagaaagtt cctgtgatgt caagctggtc gagaaaagct ttgaaacaga tacgaaccta
     5581 aactttcaaa acctgtcagt gattgggttc cgaatcctcc tcctgaaagt ggccgggttt
     5641 aatctgctca tgacgctgcg gctgtggtcc agctgaaaac gttgttgttt ggggttgaat
     5701 tactctacat gtttaagggt tccggttcca ctaggtacaa ttcgatatca agcttatcga
     5761 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     5821 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     5881 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     5941 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     6001 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     6061 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     6121 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     6181 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     6241 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     6301 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta
     6361 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     6421 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     6481 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     6541 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     6601 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc
     6661 cggcgaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     6721 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     6781 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     6841 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     6901 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     6961 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     7021 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     7081 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     7141 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     7201 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     7261 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     7321 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     7381 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     7441 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     7501 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     7561 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     7621 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     7681 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     7741 gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt
     7801 atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg
     7861 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc
     7921 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga
     7981 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt
     8041 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc
     8101 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg
     8161 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg
     8221 tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     8281 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     8341 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     8401 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     8461 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     8521 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     8581 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     8641 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     8701 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     8761 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     8821 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     8881 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     8941 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     9001 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     9061 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     9121 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     9181 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     9241 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     9301 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     9361 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     9421 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     9481 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     9541 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     9601 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     9661 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     9721 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     9781 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     9841 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     9901 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     9961 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    10021 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    10081 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    10141 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    10201 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    10261 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    10321 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    10381 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    10441 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    10501 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    10561 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    10621 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
//
