LOCUS       sequence-464914-       10700 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ncHLAp-reactive T cell receptor (TCR005H).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10700)
  AUTHORS   Ely ZA, Kulstad ZJ, Gunaydin G, Addepalli S, Verzani EK, Casarrubios
            M, Clauser KR, Wang X, Lippincott IE, Louvet C, Schmitt T, Kapner 
            KS, Agus MP, Hennessey CJ, Cleary JM, Hadrup SR, Klaeger S, Su J, 
            Jaeger AM, Wolpin BM, Raghavan S, Smith EL, Greenberg PD, Aguirre 
            AJ, Abelin JG, Carr SA, Jacks T, Freed-Pastor WA.
  TITLE     Pancreatic cancer-restricted cryptic antigens are targets for T cell
            recognition.
  JOURNAL   Science. 2025. May 8; 388(6747)
REFERENCE   2  (bases 1 to 10700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2025. May 8; 388(6747)"
COMMENT     Sequence Label: TCR005H
FEATURES             Location/Qualifiers
     source          1..10700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2638..3816
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2869..3807
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3764..3784
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4831..4887
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    5792..6380
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5845..5865)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6263..6274)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6452..6685
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6711..6728)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6717..6941
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6987..7415
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7074..7093)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7284..7305
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7424..7444)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7429..7758
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7609..7744
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7671..7690
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7806..7853
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7872..8246
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8376..8497
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8413..8432)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8467..8486
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8546..8562)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8546..8562)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8559..8581)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8570..8586
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8594..8600,8601..8618,8619..8624))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8639..8660
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8777..8794)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8948..9536)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9028..9047)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9707..10498,10499..10567))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10330..10349
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10568..10672)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aggtaccgat cagtgtgagg gagtgtaaag ctggtttggc
     2641 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
     2701 ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
     2761 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
     2821 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg
     2881 tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact
     2941 tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag
     3001 agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct
     3061 gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc
     3121 gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa
     3181 gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg
     3241 ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg
     3301 cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc
     3361 ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt
     3421 gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg
     3481 cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc
     3541 tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag
     3601 ttctagagct tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag
     3661 tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc
     3721 tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg
     3781 gttcaaagtt tttttcttcc atttcaggtg tcgtgactaa ctcgaacgct agctgtgcga
     3841 tcgtttgcca ccatgcttag tcctgacctg cctgactctg cctggaacac caggctcctc
     3901 tgccgtgtca tgctttgtct cctgggagca ggttcagtgg ctgctggagt catccagtcc
     3961 ccaagacatc tgatcaaaga aaagagggaa acagccactc tgaaatgcta tcctatccct
     4021 agacacgaca ctgtctactg gtaccagcag ggtccaggtc aggaccccca gttcctcatt
     4081 tcgttttatg aaaagatgca gagcgataaa ggaagcatcc ctgatcgatt ctcagctcaa
     4141 cagttcagtg actatcattc tgaactgaac atgagctcct tggagctggg ggactcagcc
     4201 ctgtacttct gtgccagcag cttatccggt tccgatcagc cccagcattt tggtgatggg
     4261 actcgactct ccatcctaga ggacctgaac aaggtgttcc cacccgaggt cgctgtgttt
     4321 gagccatcag aagcagagat ctcccacacc caaaaggcca cactggtgtg cctggccaca
     4381 ggcttcttcc ctgaccacgt ggagctgagc tggtgggtga atggcaaaga agtgcattct
     4441 ggggtctgca cggacccgca gcccctcaag gagcagcccg ccctcaatga ctccagatac
     4501 tgcctgagca gccgcctgag ggtctcggcc accttctggc agaacccccg caaccacttc
     4561 cgctgtcaag tccagttcta cgggctctcg gagaatgacg agtggaccca ggatagggcc
     4621 aaacccgtca cccagatcgt cagcgccgag gcctggggta gagcagactg tggctttacc
     4681 tcggtgtcct accagcaagg ggtcctgtct gccaccatcc tctatgagat cctgctaggg
     4741 aaggccaccc tgtatgctgt gctggtcagc gcccttgtgt tgatggccat ggtcaagaga
     4801 aaggatttca gagccaaaag aggaagcggc gccacaaact tctcactgct gaaacaggcc
     4861 ggcgacgtgg aagagaatcc cggacctatg aagaagctac tagcaatgat tctgtggctt
     4921 caactagacc ggttaagtgg agagctgaaa gtggaacaaa accctctgtt cctgagcatg
     4981 caggagggaa aaaactatac catctactgc aattattcaa ccacttcaga cagactgtat
     5041 tggtacaggc aggatcctgg gaaaagtctg gaatctctgt ttgtgttgct atcaaatgga
     5101 gcagtgaagc aggagggacg attaatggcc tcacttgata ccaaagcccg tctcagcacc
     5161 ctccacatca cagctgccgt gcatgacctc tctgccacct acttctgtgc cgtggacccg
     5221 atttctggtg gctacaataa gctgattttt ggagcaggga ccaggctggc tgtacaccca
     5281 tatatccaga accctgaccc tgctgtgtat cagctgcgtg atagcaaatc cagtgacaag
     5341 tctgtctgcc tattcaccga ttttgattct caaacaaatg tgtcacaaag taaggattct
     5401 gatgtgtata tcacagacaa atgcgtgcta gacatgaggt ctatggactt caagagcaac
     5461 agtgctgtgg cctggagcaa caaatctgac tttgcatgtg caaacgcctt caacaacagc
     5521 attattccag aagacacctt cttccccagc ccagaaagtt cctgtgatgt caagctggtc
     5581 gagaaaagct ttgaaacaga tacgaaccta aactttcaaa acctgtcagt gattgggttc
     5641 cgaatcctcc tcctgaaagt ggccgggttt aatctgctca tgacgctgcg gctgtggtcc
     5701 agctgaaaac gttgttgttt ggggttgaat tactctacat gtttaagggt tccggttcca
     5761 ctaggtacaa ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
     5821 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     5881 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     5941 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     6001 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     6061 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     6121 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     6181 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     6241 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     6301 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     6361 cctttgggcc gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat
     6421 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     6481 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     6541 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     6601 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     6661 tagtcagtgt ggaaaatctc tagcagggcc cggcgaaacc cgctgatcag cctcgactgt
     6721 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     6781 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     6841 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     6901 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     6961 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     7021 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     7081 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     7141 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     7201 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     7261 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     7321 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     7381 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     7441 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     7501 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     7561 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     7621 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     7681 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     7741 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
     7801 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     7861 ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
     7921 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
     7981 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
     8041 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
     8101 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
     8161 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
     8221 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
     8281 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     8341 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     8401 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     8461 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     8521 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     8581 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     8641 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     8701 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     8761 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     8821 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     8881 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     8941 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     9001 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     9061 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     9121 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     9181 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     9241 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     9301 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     9361 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     9421 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     9481 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     9541 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     9601 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     9661 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     9721 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     9781 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     9841 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     9901 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     9961 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    10021 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    10081 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    10141 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    10201 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    10261 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    10321 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    10381 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    10441 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    10501 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    10561 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    10621 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    10681 cccgaaaagt gccacctgac
//
