LOCUS       sequence-464919-        2366 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid for MoClo, encodes the NC1 promoter for K.marxianus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Thornbury M, Knoops A, Summerby-Murray I, Dhaliwal J, Johnson S, 
            Utomo JC, Joshi J, Narcross L, Remondetto G, Pouliot M, Whiteway M, 
            Martin VJJ
  TITLE     Sequencing of a Dairy Isolate Unlocks Kluyveromyces marxianus as a 
            Host for Lactose Valorization.
  JOURNAL   ACS Synth Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096.
  PUBMED    40629255
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096."
COMMENT     Sequence Label: pMTKM28
FEATURES             Location/Qualifiers
     source          1..2366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(273..307)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(378..1037)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     952..971
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1219..1807
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1708..1727
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctagttgttg ttgctagaga tctttttcag aaacggaacg aaacgaaaaa ggagagctgt
       61 gggtggtggc gatagattac cagatggtcg agaaattttt ttttgagtat atatacaggg
      121 ggtacgtttc agaattttga aaacctattc ttccttctcc ttaactcttt tattataatt
      181 attatattat tcattttaga cttaaaaaac catagaataa tattcttcag tcactcgctt
      241 aaacacaaat caaaatatgt gagaccagac caataaaaaa cgcccggcgg caaccgagcg
      301 ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa
      361 gcgagctcga tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca
      421 ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc
      481 ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag
      541 aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca gggattggct
      601 gaaacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa
      661 cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc
      721 cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta
      781 tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc
      841 aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc
      901 tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac
      961 tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca
     1021 gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat
     1081 acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgcccgatc
     1141 aatcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     1201 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     1261 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     1321 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     1381 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     1441 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     1501 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     1561 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     1621 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     1681 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     1741 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     1801 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     1861 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtagtc ggtctcaaac
     1921 gccacgcagt gtgaatggac tttcgagagt gttgttaaat cctgcgagat ttctggtgtt
     1981 tgtggtaaaa gcatgtaaag ttgtatactg aatttgtacg cagtttctaa ggcatttctt
     2041 cttgtgcaca agagcaaaag aaaaaaagca aaatttatga aatgacgaaa aactacaaaa
     2101 aataaaaaat aaaaaaaaat aagacgtatt atttttggtg gacacgcaga ccacggcgtg
     2161 caaagcaagt tgtttttgtt ttttctaatt gggcagttgg gaaacggtga aaactgtgaa
     2221 gaaaaaaaac aacatttttc tttctttatg caataccaat ttggaaatct cgcggccgtg
     2281 cgtgttttct agggcgggtg ggattttttg acaaatttag aaaagatgcc accagaaata
     2341 acttttttta gatagtgttt tttttt
//
