LOCUS       sequence-46492-e         613 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Alford SC, Abdelfattah AS, Ding Y, Campbell RE
  TITLE     A fluorogenic red fluorescent protein heterodimer.
  JOURNAL   Chem Biol. 2012 Mar 23;19(3):353-60.
  PUBMED    22444590
REFERENCE   2  (bases 1 to 613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem 
            Biol."; date: "2012-03-23"; volume: "19(3)"; pages: "353-60"
COMMENT     Sequence Label: pDDRFP-A1B1-DEVD
FEATURES             Location/Qualifiers
     source          1..613
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      107..116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(246..264)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     514..533
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 agcagagctc tctggctact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gtttaaactt aagcttgcca ccatggtgag
      121 caagagcgag gaggtcatca aagagttcat gcgcttcaag gtgcgcttgg agggctccat
      181 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
      241 gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc
      301 cccccagatc atgtacggct ccaaggcgta cgtgaagcac cccgccgacg tccccgatta
      361 catgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgcact tcgaggacgg
      421 cggtctggtg accgtgactc aggacacctc cctgcaggac ggcacgctga tctacaaggt
      481 gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga agaccttggg
      541 ctgggattat gccaccgagc gcctgtaccc cgaagacggc gtgctgaagg gcgagcttct
      601 ggggcgcctg aag
//
