LOCUS       sequence-46494-e       10592 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10592)
  AUTHORS   Longmire TA, Ikonomou L, Hawkins F, Christodoulou C, Cao Y, Jean JC,
            Kwok LW, Mou H, Rajagopal J, Shen SS, Dowton AA, Serra M, Weiss DJ, 
            Green MD, Snoeck HW, Ramirez MI, Kotton DN
  TITLE     Efficient derivation of purified lung and thyroid progenitors from 
            embryonic stem cells.
  JOURNAL   Cell Stem Cell. 2012 Apr 6;10(4):398-411.
  PUBMED    22482505
REFERENCE   2  (bases 1 to 10592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell."; date: "2012-04-6"; volume: "10(4)"; pages: "398-411"
COMMENT     Sequence Label: pHAGE hSPC-dsRed Express-W
FEATURES             Location/Qualifiers
     source          1..10592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2396..2418
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2410..2427
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2411..2427
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      6291..6300
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6297..6974
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(6427..6445)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(6477..6497)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     6695..6714
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6889..6912
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    6982..7570
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7035..7055)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7453..7464)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7645..7878
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7997..8015)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8083..8187
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8188..8256,8257..9048)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8406..8425)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9219..9807
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9708..9727
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9961..9978
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10218..10239
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10254..10259,10260..10277,10278..10284)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10292..10308
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10297..10319
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10316..10332
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10316..10332
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10382..10401
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtatg
     2221 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca
     2281 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     2341 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     2401 tcacgacgtt gtaaaacgac ggccagtgcc aagcttgaag actgctgctc tctaccacgt
     2461 tagctcccct gtggcggagg atgactgtcc taagagctca taggcacggg gcaaggggag
     2521 cctggctgtc agctgcctgg gcttctagtc ttgtgctttt tgcacaccag tccagggaac
     2581 gaagaccact ggctttaaga tgcttcccca gctgtcccca gactctgcca gcaggggatt
     2641 ctctggtctg agcttaagtt gtgttctccc agccagggat gcccctgccc tttgatgtct
     2701 ccttgctgcc acacatttag ccgccctccc catgccagct tggggggagg gaagcagtgg
     2761 gggtagggag gtggctgggg cagctgggca actgtcccca cccgtccctg gcacggctct
     2821 gcccagtaca caaagagcaa agtgaatctt gtccccaccc ctgcagctga ggggctggag
     2881 gaggaaacgg ggaggcccac acagaagggg tggccaccgt ggggctgtcc atcactcagg
     2941 gctctcagag ggagtcaacc cagaaacaga caaagagggt gagtctgggc tgtgttctta
     3001 gctagtgaga ggtcccctag aggatgaagt agatgatgct aatgaggatg actggatgtc
     3061 acacccatga tgctattagg tcctcataat agcatagtga ggtggacagc tagtacctga
     3121 cccatctcac agatgaacat actaatgcct aacaaagcag aacgactcac gctgggtccc
     3181 agagctggcc agtggaagca ctgagacctc cacatactga aggcatggac tattgaccgc
     3241 tgttggtatt ggtctcatca ttgactatca ttaagtgttg gctgtttgca tgcttcctgc
     3301 ccagtggcag gttcaaagaa gcccgcagga agcgtgctcc tttctttccc agggcccgca
     3361 attgggctgg aagatagagc aacaaaaagc gcccatgtaa ctcatgggaa cattcatgtg
     3421 tgctgaatgg caggtgaagg tgccacagag aggctgagga tttcagaggg caccatgaac
     3481 tggagtgagg tcgcagagca ggtgccattg gctcttggcc tgtttgggtg ggtggcattc
     3541 agagaggtgg aaggtcagat gcactgttca cgcctgtaat ctcagcactt tggaaggcca
     3601 aggtaggagg atcacacgag gccaggagat caacgctgca gtgagctatg aagctgtgat
     3661 tgcaccactg cactgcagct tgggtaacag agtgagaccc tgtctctaaa taattaaata
     3721 aataaaataa aaataaaacc ggagaagtgg agagggattg gaggtgggct ttcacagagg
     3781 gagaaacggc ttaagtacag gccaaaaagt gagaaggctg cagacagggc tggtaggggg
     3841 agggggaaat ttggcacacc cagctaaagg tccttctgtg tctccttctc caaggaaccc
     3901 aagaccttca cttggttggt gtgagcactc caggaggcag gcaccctccc tcagccctca
     3961 agcaagcaaa aatgggttta aaaaaagaag gagaagaagc agcagcagca gccgccacag
     4021 agcttgtgac agctacagcc taagggcaac aggcagggga gaccaaggac cagaaagagc
     4081 agaggctttt tcaaagaaag agatccctct cccagcaccc agcgatggcg gcaaacccca
     4141 cccacagtgc ctgctaagaa caagtcccac gtgagaacaa catggccccc cgagatgccc
     4201 acagggaccc cgagatgcct gcagtgcttg gctctcccgc tggccagctg cccacctggc
     4261 tcaggcccag tactcgtgag tcagccgatc aatcccaatg ttgccaggat gatgggggcg
     4321 ggagtaaggg ccctggggga gggcaggggt gggcactgca ggcgagctgt ctcccacatc
     4381 tggcacctgc acacagctga ggccgagctg agaggatgct tctgtgggct ccccctcctc
     4441 ccggcacccc tcccctcctt tcactgtcct aggacactct ctggctgctg gagtcttagg
     4501 caaatattta aaggggcagc aagggggtga ggagggtggt gggagcaaac acttccctcc
     4561 ctttcttcct ccctgcgctt ctcaggggct ctcagttcag atgccatgct gttatgcaac
     4621 cttggggctg aaggccctcc agatggagag ggggacaggg gaacctgcca gctcatgacc
     4681 gaagggcagg gcccaggtgg gagggggctg gggcagggga caggaactgg ggtggcatgt
     4741 taaaggacag gaggctggtt gggcatggtg gctcacacct gtaatcctag cactttagga
     4801 ggccgagatc acttgagccc aggagttcaa gaccagcctg ggcaacatgg tgaaaactca
     4861 tctctataaa acaagcaaaa attagctggg cacaatggca tgcactggta gtcccagcta
     4921 cttgggatgc tgaggtgtga ggatcaccgg agtccaggag gtcaacgctg cagtgagcag
     4981 tgatctcgct actgcatgcc agcctgggtg ataaagtgag accctgtctc acaacaaaac
     5041 aaaacaaaac aaaacaaaag gataggaggt taagggagcg agcccaggcc tggactctgc
     5101 cacagtcacc tgagtttaga ggtgaaggga ctttagagac cacctggccc aaggagtgaa
     5161 agggaacctg agagaaaggg tgagccagcc caaagtcatt ggcagatttg acctcgtaaa
     5221 tacatagaga tggctttggg aaggcactag gaaagacaga gaaaagagaa ggagacagtc
     5281 ctcaaagctg atcgtatttg ggtgagtatt attctcaggg caaatttagg atcagaggat
     5341 gcagaaaggg gagtctagag gggtagagtg tagaccacag ggtgagtgag ctgattcgag
     5401 gatggggaga ctgggagccc accagtgacc agagccagcc ctgttcaggg ctgtccgggc
     5461 agaagaaagc agtgtcagac ctggaatctg ccatcagcac agcctgcaat tgacagacaa
     5521 gcccagagca aagaaggaag cactgcacat gagtaagagc ttgccaccag tggggacaga
     5581 gtttccagaa ttaggaaaat aatcactggg ggcaagtttg aggttggtac cagatatgtg
     5641 ggaggaggca aggtaaggga aagagtactt gaagttggaa ctggtccttg cagggaaatg
     5701 cacatttatg aaaccccgaa aactgatgtc aaagcacctc ctgccttggg cagagtcctc
     5761 tcagagtcta caggtgctgc ctccagaacc ctcttcctgg agcgcatccc tatgtatcta
     5821 gaaattctgc tgggaaatat gatggtcaga cccttggcca cctgaaaggt tcagggtggt
     5881 agaagaaaaa ggaaagccac agggcagcag gggcaggtgc cagcaaggaa ggcaggcacg
     5941 ccaggaagac acccatggtg agaagtgcag atggcccgag ggcaagtttg ctcaactcac
     6001 ccaggtttgc tcttgctggg gccaagagga ctcatgtgcc agggccaagg gcccttgggg
     6061 gctctcacag ggggcttatc tgggcttcgg ttctggaggg ccaggaacaa acaggcttca
     6121 aagccaaggg cttggctggc acacaggggg cttggtcctt cacctctgtc ccctctccct
     6181 acggacacat ataagaccct ggtcacacct gggagaggag gagaggagag catagcacct
     6241 gcaggtcgac tctagaggat cgatccccgg gtaccgagct cgaattagcg gccgccatgg
     6301 cctcctccga ggacgtcatc aaggagttca tgcgcttcaa ggtgcgcatg gagggctccg
     6361 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     6421 agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt
     6481 ccccccagtt ccagtacggc tccaaggtgt acgtgaagca ccccgccgac atccccgact
     6541 acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     6601 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggctccttc atctacaagg
     6661 tgaagttcat cggcgtgaac ttcccctccg acggccccgt aatgcagaag aagactatgg
     6721 gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc
     6781 acaaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag tccatctaca
     6841 tggccaagaa gcccgtgcag ctgcccggct actactacgt ggactccaag ctggacatca
     6901 cctcccacaa cgaggactac accatcgtgg agcagtacga gcgcgccgag ggccgccacc
     6961 acctgttcct gtagggatcc taatcaacct ctggattaca aaatttgtga aagattgact
     7021 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     7081 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     7141 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     7201 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     7261 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     7321 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     7381 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     7441 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     7501 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     7561 gcctccccgc ctgagatcct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     7621 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     7681 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     7741 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     7801 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     7861 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     7921 caaagaaatg aatatcagag agtgagaggc ccgggttaat taaggaaagg gctagatcat
     7981 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     8041 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     8101 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     8161 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     8221 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     8281 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     8341 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     8401 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     8461 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     8521 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     8581 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     8641 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     8701 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     8761 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     8821 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     8881 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     8941 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     9001 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     9061 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     9121 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     9181 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     9241 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     9301 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     9361 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     9421 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     9481 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     9541 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     9601 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     9661 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     9721 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     9781 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     9841 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     9901 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     9961 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
    10021 cgttggccga ttcattaatg cagcaagctc atggctgact aatttttttt atttatgcag
    10081 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
    10141 gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc
    10201 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
    10261 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
    10321 aacagctatg acatgattac gaatttcaca aataaagcat ttttttcact gcattctagt
    10381 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
    10441 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
    10501 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
    10561 atttgttaaa tatgtactac aaacttagta gt
//
