LOCUS       sequence-464940-        8631 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The empty vector for quadruple sgRNA cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8631)
  AUTHORS   Yin JA, Frick L, Scheidmann MC, Liu T, Trevisan C, Dhingra A, 
            Spinelli A, Wu Y, Yao L, Vena DL, Knapp B, Guo J, De Cecco E, Ging 
            K, Armani A, Oakeley EJ, Nigsch F, Jenzer J, Haegele J, Pikusa M, 
            Tager J, Rodriguez-Nieto S, Bouris V, Ribeiro R, Baroni F, Bedi MS, 
            Berry S, Losa M, Hornemann S, Kampmann M, Pelkmans L, Hoepfner D, 
            Heutink P, Aguzzi A
  TITLE     Arrayed CRISPR libraries for the genome-wide activation, deletion 
            and silencing of human protein-coding genes.
  JOURNAL   Nat Biomed Eng. 2024 Dec 4. doi: 10.1038/s41551-024-01278-4.
  PUBMED    39633028
REFERENCE   2  (bases 1 to 8631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Biomed 
            Eng. 2024 Dec 4. doi: 10.1038/s41551-024-01278-4."
COMMENT     Sequence Label: pYJA5
FEATURES             Location/Qualifiers
     source          1..8631
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    383..508
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             555..735
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(753..951)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(778..798)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        953..1256
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(1326..1345)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1327..1345)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1355..1372)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1355..1371)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1364..1386)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1513..1968
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1600..1619)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1810..1831
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      2122..2710
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2611..2630
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2864..2881
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2998..3019
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3034..3039,3040..3057,3058..3064)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3072..3088
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3077..3099
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3096..3112
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3096..3112
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3131..3151
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3133..3151
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   3171..3205
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    3492..3716
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3705..3722
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(4077..4778)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             complement(4779..4832)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(4851..5447)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4931..4951)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     5428..5447
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(5487..5985)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5535..5554)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     5927..5950
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     promoter        6018..6266
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6018..6038
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6189..6208
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        6276..6380
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6381..6449,6450..7241)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6599..6618)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     repeat_region   7513..7575
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     misc_feature    7640..7757
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(8055..8099)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8284..8517
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 tccctcatat ctcctcctcc aggtctgaag atcagcggcc ggccgcttgc tgtgcggtgg
       61 tcttactttt gttttgctct tcctctatct tgtctaaagc ttccttggtg tcttttatct
      121 ctatcctttg atgcacacaa tagagggttg ctactgtatt atataatgat ctaagttctt
      181 ctgatcctgt ctgaagggat ggttgtagct gtcccagtat ttgtctacag ccttctgatg
      241 tttctaacag gccaggatta actgcgaatc gttctagctc cctgcttgcc catactatat
      301 gttttaattt atattttttc tttccccctg gccttaaccg aattttttcc catcgcgatc
      361 taattctccc ccgcttaata ctgacgctct cgcacccatc tctctccttc tagcctccgc
      421 tagtcaaaat ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc gtgcgcgctt
      481 cagcaagccg agtcctgcgt cgagagagct cctctggttt ccctttcgct ttcaagtccc
      541 tgttcgggcg ccactgctag agattttcca cactgactaa aagggtctga gggatctcta
      601 gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta
      661 ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc
      721 taaccagaga gacccagtac aggcaaaacg cgctgcttat atagacctcc caccgtacac
      781 gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt
      841 gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc
      901 gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa
      961 tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact
     1021 gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca
     1081 tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac
     1141 gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg
     1201 ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga
     1261 actccatata tgggctatga actaatgacc ccgtaattga ttactattaa taactagtca
     1321 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     1381 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     1441 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     1501 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     1561 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     1621 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     1681 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     1741 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     1801 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     1861 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     1921 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     1981 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     2041 tccgctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     2101 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     2161 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     2221 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     2281 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     2341 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     2401 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     2461 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     2521 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     2581 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     2641 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     2701 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     2761 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     2821 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     2881 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     2941 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     3001 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgctcc cggctcgtat
     3061 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     3121 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag cttaatttaa ccctagaaag
     3181 atagtctgcg taaaattgac gcatgcattc ttgaaatatt gctctctctt tctaaatagc
     3241 gcgaatccgt cgctgtgcat ttaggacatc tcagtcgccg cttggagctc ccgtgaggcg
     3301 tgcttgtcaa tgcggtaagt gtcactgatt ttgaactata acgaccgcgt gagtcaaaat
     3361 gacgcatgat tatcttttac gtgactttta agatttaact catacgataa ttatattgtt
     3421 atttcatgtt ctacttacgt gataacttat tatatatata ttttcttgtt atagatatca
     3481 ttaacggccg cccatagagc ccaccgcatc cccagcatgc ctgctattga cttcccaatc
     3541 ctcccccttg ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca
     3601 atgcgatgca atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt
     3661 caaggaaggc acgggggagg ggcaaacaac agatggctgg caactagaag gcacagtcga
     3721 ggctgatcag cgggtttaaa ctgctagaga ttttccacac tgactaaaag ggtctgaggg
     3781 atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca
     3841 agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga
     3901 tctggtctaa ccagagagac tctggtctaa ccagagagac ccagtacagg caaaaagcag
     3961 atcttgactt ctttgggagt gaattagccc ttccagtccc cccttttctt ttaaaaagtg
     4021 gctaagatct acagctgcct tgtaagtcat tggtcttaaa ggtacctagc ggccgctcaa
     4081 ttaagcttgt gccccagttt gctagggagg tcgcagtatc tggccactgc cacctcgtgc
     4141 tgctcgacgt aggtctcgtt gttggcctcc ttgattcttt ccagtctgta gtccacatag
     4201 tagacgccag gcatcttgag gttcttagcg ggtttcttgg atctatatgt ggtcttgatg
     4261 tttgcgatca gatggctccc gcccacgagc ttcagggcca tgtcgtttct gccttccagg
     4321 ccgccgtcag cggggtacag agtctcggtg aaggcctccc agccgagtgt tttcttctgc
     4381 atcacagggc cgttggatgt gaagttcacc cctctgatct tgacgttgta gatgaggcag
     4441 ccgtcctgga ggctggtgtc ctgggtagcg gtcagcacgc ccccgtcctc gtatgtggtg
     4501 actctctccc atgtgaagcc ctcagggaag gactgcttga agaagtcggg gatgccctgg
     4561 gtgtggttga tgaaggtctt gctgccgtag aggaagctag tagccaggat gtcgaaggcg
     4621 aaggggagag ggccgccctc gaccaccttg attctcatgg tctgggtgcc ctcgtagggc
     4681 ttgccttcgc cctcggatgt gcacttgaag tgatggttgt ccacggtgcc ctccatgtac
     4741 agcttcatgt gcatgttctc cttaatcagc tcgctcattg ggccaggatt ctcctccacg
     4801 tcaccgcatg ttaggagact tcctctgccc tctcctccgg acccgccgcc ggcaccgggc
     4861 ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg
     4921 aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg aggtctccag gaaggcgggc
     4981 accccggcgc gctcggccgc ctccactccg gggagcacga cggcgctgcc cagacccttg
     5041 ccctggtggt cgggcgagac cccgacggtg gccaggaacc acgcgggctc cttgggccgg
     5101 tgcggcgcca ggaggccttc catctgttgc tgcgcggcca gccgggaacc gctcaactcg
     5161 gccatgcgcg ggccgatctc ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc
     5221 cagaccgcca ccgcggcgcc gtcgtccgcg acccacacct tgccgatgtc gagcccgacg
     5281 cgcgtgagga agagttcttg cagctcggtg acccgctcga tgtggcggtc cggatcgacg
     5341 gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga gggtgcgtac ggccctgggg
     5401 acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact cggtcatggt aagcttcagc
     5461 tgctcgagat ctagatggat gcaggtcgaa aggcccggag atgaggaaga ggagaacagc
     5521 gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga ccttcgggcg
     5581 cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct tcccagcctc
     5641 tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag gcctacccgc
     5701 ttccattgct cagcggtgct gtccatctgc acgagactag tgagactgct acttccattt
     5761 gtcacgtcct gcacgacgcg agctgcgggg cgggggggaa cttcctgact aggggaggag
     5821 tagaaggtgg cgcgaagggg ccaccaaaga acggagccgg ttggcgccta ccggtggatg
     5881 tggaatgtgt gcgaggccag aggccacttg tgtagcgcca agtgcccagc ggggctgcta
     5941 aagcgcatgc tccagactgc cttgggaaaa gcgcctcccc tacccggtag aattggatcc
     6001 ccaaggtcgg gcaggaagag ggcctatttc ccatgattcc ttcatatttg catatacgat
     6061 acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag atattagtac
     6121 aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt
     6181 ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat
     6241 atatcttgtg gaaaggacga aacaccgggg tcttccgcgg aacccctatt tgtttatttt
     6301 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     6361 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     6421 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     6481 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     6541 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     6601 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     6661 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     6721 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     6781 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     6841 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     6901 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     6961 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     7021 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     7081 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     7141 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     7201 agatcgctga gataggtgcc tcactgatta agcattggta agaagacggg tttcagagct
     7261 acagcagaaa tgctgtagca agttgaaata aggctagtcc gttatcaact tgaaaaagtg
     7321 gcaccgagtc ggtgcttttt ttgctttgtt actttataga agaaattttg agtttttgtt
     7381 tttttttaat aaataaataa acataaataa attgtttgtt gaatttatta ttagtatgta
     7441 agtgtaaata taataaaact taatatctat tcaaattaat aaataaacct cgatatacag
     7501 accgataaaa cacatgcgtc aattttacgc atgattatct ttaacgtacg tcacaatatg
     7561 attatctttc tagggttaag taacgcgtag ggcccggtac taaccaaact ggatctctgc
     7621 tgtccctgta ataaacccga aaattttgaa tttttgtaat ttgtttttgt aattctttag
     7681 tttgtatgtc tgttgctatt atgtctacta ttctttcccc tgcactgtac cccccaatcc
     7741 ccccttttct tttaaaattg tggatgaata ctgccatttg tctgcagaat tggcgcacgc
     7801 agtgccgatc cgttcactaa tcgaatggat ctgtctctgt ctctctctcc accttcttct
     7861 tctattcctt cgggcctgtc gggtcccctc ggggttggga ggtgggtctg aaacgataat
     7921 ggtgaatatc cctgcctaac tctattcact atagaaagta cagcaaaaac tattcttaaa
     7981 cctaccaagc ctcctactat cattatgaat aattttatat accacagcca atttgttatg
     8041 ttaaaccaat tccacaaact tgcccattta tctaattcca ataattcttg ttcattcttt
     8101 tcttgctggt tttgcgattc ttcaattaag gagtgtatta agcttgtgta attgttaatt
     8161 tctctgtccc actccatcca ggtcgtgtga ttccaaatct gttccagaga tttattactc
     8221 caactagcat tccaaggcac agcagtggtg caaatgagtt ttccagagca accccaaatc
     8281 cccaggagct gttgatcctt taggtatctt tccacagcca ggattcttgc ctggagctgc
     8341 ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg cctcaatagc cctcagcaaa
     8401 ttgttctgct gctgcactat accagacaat aattgtctgg cctgtaccgt cagcgtcatt
     8461 gacgctgcgc ccatagtgct tcctgctgct cccaagaacc caaggaacaa agctcctatt
     8521 cccactgctc ttttttctct ctgcaccact cttctctttg ccttggtggg tgctactcct
     8581 aatggttcaa tttttactac tttatattta tataattcac ttctccaatt g
//
