LOCUS       sequence-464941-       11099 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rhamnose inducible expression of Bambusa oldhamii PAL gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11099)
  AUTHORS   Hunstiger D, Ma H, Paton AJ, Peebles CAM
  TITLE     Improving trans-cinnamic acid production in a model cyanobacterium
  JOURNAL   Biotechnol. Prog. 2024;e3512.
REFERENCE   2  (bases 1 to 11099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Prog. 2024;e3512."
COMMENT     Sequence Label: pDF1b-iBOP
FEATURES             Location/Qualifiers
     source          1..11099
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     193..215
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        285..362
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             complement(361..1197)
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
     protein_bind    1241..1257
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    1274..1290
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      3606..3633
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(3740..3757)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3910..3996
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4088..4115
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      4594..4988
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            5330..5417
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             6656..7627
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             8138..8977
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             8964..9815
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     terminator      complement(10044..10138)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(10182..10973)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
       61 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gcttacgaac
      121 cgaacaggct tatgtccact gggttcgtgt gtacactccc ggcatccgct tacagacaag
      181 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
      241 cgaggcagca gatcaattcg cgcgcgaagg cgaagcggca cgttgacacc atcgaatggt
      301 gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga
      361 ttattgcaga aagccatccc gtccctggcg aatatcacgc ggtgaccagt taaactctcg
      421 gcgaaaaagc gtcgaaaagt ggttactgtc gctgaatcca cagcgatagg cgatgtcagt
      481 aacgctggcc tcgctgtggc gtagcagatg tcgggctttc atcagtcgca ggcggttcag
      541 gtatcgctga ggcgtcagtc ccgtttgctg cttaagctgc cgatgtagcg tacgcagtga
      601 aagagaaaat tgatccgcca cggcatccca attcacctca tcggcaaaat ggtcctccag
      661 ccaggccaga agcaagttga ggcgtgatgc gctgttttcc aggttctcct gcaaactgct
      721 tttacgcagc aagagcagta attgcataaa caagatctcg cgactggcgg tcgagggtaa
      781 atcattttcc ccttcctgct gttccatctg tgcaaccagc tgtcgaacct gctgcaatac
      841 gctgtggtta acgcgccagt gggacggata ctgcccatcc agctcttgtg gcagcaactg
      901 attcagcccg gcgagaaact gaaatcgatc cggcgagcga tacagcacat tggtcagaca
      961 cagattatcg gtatgttcat acagatgccg atcatgatcg cgtacgaaac agaccgtgcc
     1021 accggtgatg gtatagggct gcccattaaa cacatgaata cccgtgccat gttcgacaat
     1081 cacaatttca tgaaaatcat gatgatgttc aggaaaatcc gcctgcggga gccggggttc
     1141 tatcgccacg gacgcgttac cagacggaaa aaaatccaca ctatgtaata cggtcatact
     1201 ggcctcctga tgtcgtcaac acggcgaaat atgtgtgaaa ttgttatccg ctcacaattg
     1261 aatctaagta tcattgttat ccgctcacaa gtcaacactc tttccacaat tcagcaaatt
     1321 gtgaacatca tcacgttcat ctttccctgg ttgccaatgg cccattttcc tgtcagtaac
     1381 gagaaggtcg cgtattcagg cgctttttag actggtcgta atgaatcggg taagtttata
     1441 atatacaaag gaggtagaaa tgcctcggga agatggccat gtagctgcga atgggaacgg
     1501 gttgtgtatg gccgcccctc gcgcagaccc tttaaattgg ggtaaagctg cagaggagct
     1561 aatgggctcc cacctcgatg aagtgaagcg tatggttgca gaatatcgcc aacccgttgt
     1621 gaagatcgag ggggcctctt tgcggatcgc ccaggtggcg gcagtagcag tagctggcga
     1681 cgcaaaagtg gaattggacg aatccgctcg agagcgagtc aaggcctcta gcgattgggt
     1741 aatgaatagc atgatgaatg ggacagattc ctacggagta actaccgggt tcggcgcgac
     1801 tagccatcga cgaaccaagg aggggggagc cttacagcgg gaactcatcc gttttttaaa
     1861 cgctggtgca tttggcacag gctgtgacgg gcatgttcta cccgccgaag ctacgcgcgc
     1921 tgcgatgttg gtgcgaatca acactctcct gcagggttac agcggaattc ggttcgagat
     1981 tttagaagca attaccaagc tcctcaatgc gaatgtaaca ccgtgtttac ctctacgcgg
     2041 tacagtaact gctagcgggg acttagtgcc gctaagctat attgccggct tggtaaccgg
     2101 acgcgaaaac tccgttgccg tcgcccctga cggacggaaa gttaatgcgg ccgaggcgtt
     2161 taaaattgcg gggatccaag gggggttctt tgaactgcag cctaaggagg gattagccat
     2221 ggtgaacgga acggcagtcg gtagcgggct ggcaagtact gtcctcttcg aggctaacat
     2281 cctggcaatt ttagctgaag ttttgagtgc ggtcttctgt gaagtgatga acggaaagcc
     2341 ggaatacaca gatcatctaa cccataagct aaaacaccac cctggccaaa ttgaggctgc
     2401 tgctattatg gagcacattt tggaaggttc cagttacatg aagctcgcta agaaactagg
     2461 tgatttggac ccactcatga aacccaaaca ggatcggtac gctctgcgaa cgtctcccca
     2521 atggctagga ccacagattg aggtgatccg tgctgccacg aagtctattg agcgtgaaat
     2581 caattctgtg aatgacaatc ccttgatcga cgtctctcga aacaaggcgt tacacggagg
     2641 taatttccaa gggacgccca ttggggtttc catggacaac actcgactag cgattgcagc
     2701 tattggaaaa ttgatgtttg ctcagtttag tgaactcgta aacgactttt ataacaacgg
     2761 gctacctagc aacttaagcg gtggtcgaaa cccgtcttta gactatggct tcaaaggggc
     2821 cgagatcgcg atggccagct attgcagcga gctgcagttc ttgggtaacc ctgttacgaa
     2881 tcatgtacag agcgctgaac aacataacca agatgtgaat agcctaggcc taattagctc
     2941 tcgtaagacg gccgaagcca ttgatatttt aaagattatg agctctacat ttctagtcgc
     3001 attgtgtcag gctattgatc tccgacatat tgaggagaac gtaaagagcg ctgttaaaag
     3061 ttgtgttatg actgttgcca agaagacgcc ctccacaaac agcacaggtg acctgcacgt
     3121 agctcggttc tgtgaaaaag atctcctaaa agaaatcgac cgcgaggcgg tttttgcata
     3181 tgcagatgat ccctgctccc cgaattatcc cctaatgaag aagctacgaa gtgtcttggt
     3241 tgagagcgcg ttagcgaacg gtatggcaga atttaatgcg gagacatcca tcttcgctcg
     3301 cgtggctctc ttcgaagagg agttgcgtgc agccctccct cgtgctgtgg aggccgctcg
     3361 cgcgagcgtg gaaaacggga ctgcagcggc acccaaccgc atcaccgaat gtcgttctta
     3421 tccattatac cggttcgttc gggaagaact aggtacagaa tacctcacgg gcgagaagac
     3481 acgctcccct ggggaggagc tgaataaagt attgttggcc attaaccaag gaaaacatat
     3541 cgacccctta ctcgaatgtt tgaaagaatg gaatggtgag ccgctcccta tctgttgatc
     3601 acactggctc accttcgggt gggcctttct gcgtttatat acgagagaga gaatataaaa
     3661 agccagatta ttaatccggc ttttttatta ttttactaga gaagcttggc tgttttggcg
     3721 gatgagagaa gattttcagc ctgatacaga ttaaatcaga acgcagaagc ggtctgataa
     3781 aacagaattt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg ccgaactcag
     3841 aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga gtagggaact
     3901 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     3961 tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt
     4021 gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggcatcaa
     4081 attaagcaga aggccatcct gacggatggc ctttttgcgt ttctacaaac tcttttgttt
     4141 atttttctaa atacattcaa atatgtatcc gctcctaggg gatcccatgg tacgctagct
     4201 gaaagcgacc aggtgctcgg cgtggcaaga ctcgcagcga acccgtagaa agccatgctc
     4261 cagccgcccg cattggagaa attcttcaaa ttcccgttgc acatagcccg gcaattcctt
     4321 tccctgctct gccataagcg cagcgaatgc cgggtaatac tcgtcaacga tctgatagag
     4381 aagggtttgc tcgggtcggt ggctctggta acgaccagta tcccgatccc ggctggccgt
     4441 cctggccgcc acatgaggca tgttccgcgt ccttgcaata ctgtgtttac atacagtcta
     4501 tcgcttagcg gaaagttctt ttaccctcag ccgaaatgcc tgccgttgct agacattgcc
     4561 agccagtgcc cgtcactccc gtactaactg tcacgaaccc ctgcaataac tgtcacgccc
     4621 ccctgcaata actgtcacga acccctgcaa taactgtcac gcccccaaac ctgcaaaccc
     4681 agcaggggcg ggggctggcg gggtgttgga aaaatccatc catgattatc taagaataat
     4741 ccactaggcg cggttatcag cgcccttgtg gggcgctgct gcccttgccc aatatgcccg
     4801 gccagaggcc ggatagctgg tctattcgct gcgctaggct acacaccgcc ccaccgctgc
     4861 gcggcagggg gaaaggcggg caaagcccgc taaaccccac accaaacccc gcagaaatac
     4921 gctggagcgc ttttagccgc tttagcggcc tttcccccta cccgaagggt gggggcgcgt
     4981 gtgcagcccc gcagggcctg tctcggtcga tcattcagcc cggctcatcc ttctggcgtg
     5041 gcggcagacc gaacaaggcg cggtcgtggt cgcgttcaag gtacgcatcc attgccgcca
     5101 tgagccgatc ctccggccac tcgctgctgt tcaccttggc caaaatcatg gcccccacca
     5161 gcaccttgcg ccttgtttcg ttcttgcgct cttgctgctg ttcccttgcc cgcacccgct
     5221 gaatttcggc attgattcgc gctcgttgtt cttcgagctt ggccagccga tccgccgcct
     5281 tgttgctccc cttaaccatc ttgacacccc attgttaatg tgctgtctcg taggctatca
     5341 tggaggcaca gcggcggcaa tcccgaccct actttgtagg ggagggcgca cttaccggtt
     5401 tctcttcgag aaactggcct aacggccacc cttcgggcgg tgcgctctcc gagggccatt
     5461 gcatggagcc gaaaagcaaa agcaacagcg aggcagcatg gcgatttatc accttacggc
     5521 gaaaaccggc agcaggtcgg gcggccaatc ggccagggcc aaggccgact ttatccagcg
     5581 cgaaggcaag tatgcccgcg acatggatga agtcttgcac gccgaatccg ggcacatgcc
     5641 ggagttcgtc gagcggcccg ccgactactg ggatgctgcc gacctgtatg aacgcgccaa
     5701 tgggcggctg ttcaaggagg tcgaatttgc cctgccggtc gagctgaccc tcgaccagca
     5761 gaaggcgctg gcgtccgagt tcgcccagca cctgaccggt gccgagcgcc tgccgtatac
     5821 gctggccatc catgccggtg gcggcgagaa cccgcactgc cacctgatga tctccgagcg
     5881 gatcaatgac ggcatcgagc ggcccgccgc tcagtggttc aagcggtaca acggcaagac
     5941 cccggagaag ggcggggcac agaagaccga agcgctcaag cccaaggcat ggcttgagca
     6001 gacccgcgag gcatgggccg accatgccaa ccgggcatta gagcgggctg gccacgacgc
     6061 ccgcattgac cacagaacac ttgaggcgca gggcatcgag cgcctgcccg gtgttcacct
     6121 ggggccgaac gtggtggaga tggaaggccg gggcatccgc accgaccggg cagacgtggc
     6181 cctgaacatc gacaccgcca acgcccagat catcgactta caggaatacc gggaggcaat
     6241 agaccatgaa cgcaatcgac agagtgaaga aatccagagg catcaacgag ttagcggagc
     6301 agatcgaacc gctggcccag agcatggcga cactggccga cgaagcccgg caggtcatga
     6361 gccagaccca gcaggccagc gaggcgcagg cggcggagtg gctgaaagcc cagcgccaga
     6421 caggggcggc atgggtggag ctggccaaag agttgcggga ggtagccgcc gaggtgagca
     6481 gcgccgcgca gagcgcccgg agcgcgtcgc gggggtggca ctggaagcta tggctaaccg
     6541 tgatgctggc ttccatgatg cctacggtgg tgctgctgat cgcatcgttg ctcttgctcg
     6601 acctgacgcc actgacaacc gaggacggct cgatctggct gcgcttggtg gcccgatgaa
     6661 gaacgacagg actttgcagg ccataggccg acagctcaag gccatgggct gtgagcgctt
     6721 cgatatcggc gtcagggacg ccaccaccgg ccagatgatg aaccgggaat ggtcagccgc
     6781 cgaagtgctc cagaacacgc catggctcaa gcggatgaat gcccagggca atgacgtgta
     6841 tatcaggccc gccgagcagg agcggcatgg tctggtgctg gtggacgacc tcagcgagtt
     6901 tgacctggat gacatgaaag ccgagggccg ggagcctgcc ctggtagtgg aaaccagccc
     6961 gaagaactat caggcatggg tcaaggtggc cgacgccgca ggcggtgaac ttcgggggca
     7021 gattgcccgg acgctggcca gcgagtacga cgccgacccg gccagcgccg acagccgcca
     7081 ctatggccgc ttggcgggct tcaccaaccg caaggacaag cacaccaccc gcgccggtta
     7141 tcagccgtgg gtgctgctgc gtgaatccaa gggcaagacc gccaccgctg gcccggcgct
     7201 ggtgcagcag gctggccagc agatcgagca ggcccagcgg cagcaggaga aggcccgcag
     7261 gctggccagc ctcgaactgc ccgagcggca gcttagccgc caccggcgca cggcgctgga
     7321 cgagtaccgc agcgagatgg ccgggctggt caagcgcttc ggtgatgacc tcagcaagtg
     7381 cgactttatc gccgcgcaga agctggccag ccggggccgc agtgccgagg aaatcggcaa
     7441 ggccatggcc gaggccagcc cagcgctggc agagcgcaag cccggccacg aagcggatta
     7501 catcgagcgc accgtcagca aggtcatggg tctgcccagc gtccagcttg cgcgggccga
     7561 gctggcacgg gcaccggcac cccgccagcg aggcatggac aggggcgggc cagatttcag
     7621 catgtagtgc ttgcgttggt actcacgcct gttatactat gagtactcac gcacagaagg
     7681 gggttttatg gaatacgaaa aaagcgcttc agggtcggtc tacctgatca aaagtgacaa
     7741 gggctattgg ttgcccggtg gctttggtta tacgtcaaac aaggccgagg ctggccgctt
     7801 ttcagtcgct gatatggcca gccttaacct tgacggctgc accttgtcct tgttccgcga
     7861 agacaagcct ttcggccccg gcaagtttct cggtgactga tatgaaagac caaaaggaca
     7921 agcagaccgg cgacctgctg gccagccctg acgctgtacg ccaagcgcga tatgccgagc
     7981 gcatgaaggc caaagggatg cgtcagcgca agttctggct gaccgacgac gaatacgagg
     8041 cgctgcgcga gtgcctggaa gaactcagag cggcgcaggg cgggggtagt gaccccgcca
     8101 gcgcctaacc accaactgcc tgcaaaggag gcaatcaatg gctacccata agcctatcaa
     8161 tattctggag gcgttcgcag cagcgccgcc accgctggac tacgttttgc ccaacatggt
     8221 ggccggtacg gtcggggcgc tggtgtcgcc cggtggtgcc ggtaaatcca tgctggccct
     8281 gcaactggcc gcacagattg caggcgggcc ggatctgctg gaggtgggcg aactgcccac
     8341 cggcccggtg atctacctgc ccgccgaaga cccgcccacc gccattcatc accgcctgca
     8401 cgcccttggg gcgcacctca gcgccgagga acggcaagcc gtggctgacg gcctgctgat
     8461 ccagccgctg atcggcagcc tgcccaacat catggccccg gagtggttcg acggcctcaa
     8521 gcgcgccgcc gagggccgcc gcctgatggt gctggacacg ctgcgccggt tccacatcga
     8581 ggaagaaaac gccagcggcc ccatggccca ggtcatcggt cgcatggagg ccatcgccgc
     8641 cgataccggg tgctctatcg tgttcctgca ccatgccagc aagggcgcgg ccatgatggg
     8701 cgcaggcgac cagcagcagg ccagccgggg cagctcggta ctggtcgata acatccgctg
     8761 gcagtcctac ctgtcgagca tgaccagcgc cgaggccgag gaatggggtg tggacgacga
     8821 ccagcgccgg ttcttcgtcc gcttcggtgt gagcaaggcc aactatggcg caccgttcgc
     8881 tgatcggtgg ttcaggcggc atgacggcgg ggtgctcaag cccgccgtgc tggagaggca
     8941 gcgcaagagc aagggggtgc cccgtggtga agcctaagaa caagcacagc ctcagccacg
     9001 tccggcacga cccggcgcac tgtctggccc ccggcctgtt ccgtgccctc aagcggggcg
     9061 agcgcaagcg cagcaagctg gacgtgacgt atgactacgg cgacggcaag cggatcgagt
     9121 tcagcggccc ggagccgctg ggcgctgatg atctgcgcat cctgcaaggg ctggtggcca
     9181 tggctgggcc taatggccta gtgcttggcc cggaacccaa gaccgaaggc ggacggcagc
     9241 tccggctgtt cctggaaccc aagtgggagg ccgtcaccgc tgatgccatg gtggtcaaag
     9301 gtagctatcg ggcgctggca aaggaaatcg gggcagaggt cgatagtggt ggggcgctca
     9361 agcacataca ggactgcatc gagcgccttt ggaaggtatc catcatcgcc cagaatggcc
     9421 gcaagcggca ggggtttcgg ctgctgtcgg agtacgccag cgacgaggcg gacgggcgcc
     9481 tgtacgtggc cctgaacccc ttgatcgcgc aggccgtcat gggtggcggc cagcatgtgc
     9541 gcatcagcat ggacgaggtg cgggcgctgg acagcgaaac cgcccgcctg ctgcaccagc
     9601 ggctgtgtgg ctggatcgac cccggcaaaa ccggcaaggc ttccatagat accttgtgcg
     9661 gctatgtctg gccgtcagag gccagtggtt cgaccatgcg caagcgccgc cagcgggtgc
     9721 gcgaggcgtt gccggagctg gtcgcgctgg gctggacggt aaccgagttc gcggcgggca
     9781 agtacgacat cacccggccc aaggcggcag gctgaccccc cccactctat tgtaaacaag
     9841 acatttttat cttttatatt caatggctta ttttcctgct aattggtaat accatgaaaa
     9901 ataccatgct cagaaaaggc ttaacaatat tttgaaaaat tgcctactga gcgctgccgc
     9961 acagctccat aggccgcttt cctggctttg cttccagatg tatgctcttc tgctcctgcc
    10021 ggccgactgt ccctagtgct tggattctca ccaataaaaa acgcccggcg gcaaccgagc
    10081 gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca
    10141 agcgagctcg atatccgtcg gcttgaacga attgttagac attatttgcc gactaccttg
    10201 gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag
    10261 cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg
    10321 gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt
    10381 actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag
    10441 tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca
    10501 ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt
    10561 gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca
    10621 agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac
    10681 ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct
    10741 ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca
    10801 agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc
    10861 actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg
    10921 ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttccct catgatgttt
    10981 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctcca taacatcaaa
    11041 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacccc
//
