LOCUS       sequence-464942-       11102 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rhamnose inducible expression of Rhodotorula glutinis RgPAL gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11102)
  AUTHORS   Hunstiger D, Ma H, Paton AJ, Peebles CAM
  TITLE     Improving trans-cinnamic acid production in a model cyanobacterium
  JOURNAL   Biotechnol. Prog. 2024;e3512.
REFERENCE   2  (bases 1 to 11102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Prog. 2024;e3512."
COMMENT     Sequence Label: pDF1b-iRGP
FEATURES             Location/Qualifiers
     source          1..11102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     193..215
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        285..362
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             complement(361..1197)
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
     protein_bind    1241..1257
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    1274..1290
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      3609..3636
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(3743..3760)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3913..3999
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4091..4118
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      4597..4991
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            5333..5420
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             6659..7630
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             8141..8980
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             8967..9818
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     terminator      complement(10047..10141)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(10185..10976)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
       61 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gcttacgaac
      121 cgaacaggct tatgtccact gggttcgtgt gtacactccc ggcatccgct tacagacaag
      181 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
      241 cgaggcagca gatcaattcg cgcgcgaagg cgaagcggca cgttgacacc atcgaatggt
      301 gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga
      361 ttattgcaga aagccatccc gtccctggcg aatatcacgc ggtgaccagt taaactctcg
      421 gcgaaaaagc gtcgaaaagt ggttactgtc gctgaatcca cagcgatagg cgatgtcagt
      481 aacgctggcc tcgctgtggc gtagcagatg tcgggctttc atcagtcgca ggcggttcag
      541 gtatcgctga ggcgtcagtc ccgtttgctg cttaagctgc cgatgtagcg tacgcagtga
      601 aagagaaaat tgatccgcca cggcatccca attcacctca tcggcaaaat ggtcctccag
      661 ccaggccaga agcaagttga ggcgtgatgc gctgttttcc aggttctcct gcaaactgct
      721 tttacgcagc aagagcagta attgcataaa caagatctcg cgactggcgg tcgagggtaa
      781 atcattttcc ccttcctgct gttccatctg tgcaaccagc tgtcgaacct gctgcaatac
      841 gctgtggtta acgcgccagt gggacggata ctgcccatcc agctcttgtg gcagcaactg
      901 attcagcccg gcgagaaact gaaatcgatc cggcgagcga tacagcacat tggtcagaca
      961 cagattatcg gtatgttcat acagatgccg atcatgatcg cgtacgaaac agaccgtgcc
     1021 accggtgatg gtatagggct gcccattaaa cacatgaata cccgtgccat gttcgacaat
     1081 cacaatttca tgaaaatcat gatgatgttc aggaaaatcc gcctgcggga gccggggttc
     1141 tatcgccacg gacgcgttac cagacggaaa aaaatccaca ctatgtaata cggtcatact
     1201 ggcctcctga tgtcgtcaac acggcgaaat atgtgtgaaa ttgttatccg ctcacaattg
     1261 aatctaagta tcattgttat ccgctcacaa gtcaacactc tttccacaat tcagcaaatt
     1321 gtgaacatca tcacgttcat ctttccctgg ttgccaatgg cccattttcc tgtcagtaac
     1381 gagaaggtcg cgtattcagg cgctttttag actggtcgta atgaatcggg taagtttata
     1441 atatacaaag gaggtagaaa tggcacctag cgttgacagc atcgcaacat ctgtggctaa
     1501 ttccttatcc aacggattgc atgcggcagc cgctgctaac ggtggggacg tccataaaaa
     1561 gacggcgggg gcagggtctc tcttacccac gacggagaca acgcagttag acattgttga
     1621 acgaattcta gccgatgccg gcgctactga ccaaattaag ctagacggtt acaccttgac
     1681 cttaggcgac gttgttgggg ctgcccgtcg cggtcggtcc gtaaaggttg cggatagccc
     1741 acacattcga gaaaaaattg acgcctccgt tgaattttta cggacacagc tagacaacag
     1801 cgtatatggg gtgacgactg gtttcggcgg cagcgctgac acgcgcaccg aagacgccat
     1861 tagcttacag aaagcactac tcgagcatca gttatgtggg gtactcccaa cgtctatgga
     1921 cggatttgca ctagggcggg ggctcgagaa ctctctgcct ctcgaagtcg tgcgcggtgc
     1981 gatgactatc cgcgtaaact ctctcacacg tggtcattcc gctgtgcgta ttgtagtact
     2041 cgaagcattg actaacttcc taaaccacgg catcactcca atcgttcctt tacgcggcac
     2101 aatttctgca tccggtgact tatctccact gagttatatc gcggctagca ttaccgggca
     2161 cccagactct aaggtgcatg tcgatggcaa gattatgtct gcacaagaag ccatcgcctt
     2221 aaagggctta caaccggttg tattgggccc aaaagaagga ttgggtttag tgaatggcac
     2281 tgccgtatct gcaagtatgg cgacgttggc tctcacggat gcccatgtcc tgagcttgct
     2341 cgctcaagcc ctgacggccc taacggttga agccatggtc ggacatgcag gtagcttcca
     2401 tccatttcta cacgacgtta cacgtccgca cccgactcaa attgaagtcg cgcgcaatat
     2461 tcgtacatta ctagaagggt ctaaatatgc tgttcatcac gagaccgaag ttaaagtcaa
     2521 agatgatgag gggattctcc gacaagaccg ctatcccctg cgttgctctc cacagtggtt
     2581 agggccactg gtcagcgaca tgattcatgc acatgctgtc ctctccttag aagcggggca
     2641 atctacgacg gacaatcctc tgattgactt agagaataaa atgacgcatc acggcggagc
     2701 gtttatggca tcttccgtag gtaacactat ggaaaagact cgcctggcag tagcattaat
     2761 gggcaaggtt tcctttacac agttaaccga gatgttaaac gcaggtatga accgtgcgtt
     2821 accgtcttgc ctggcagcgg aagatccgtc tctgtcttac cactgcaagg gactagacat
     2881 tgccgcagcg gcatacacct ccgaattggg ccatttagcc aatcctgtgt ctacccatgt
     2941 ccagccggct gaaatgggaa accaagctat caattctcta gctttaatta gtgcacgtcg
     3001 aactgcggaa gcaaatgatg tactcagttt actcctagcg acccacttat attgtgtcct
     3061 acaagccgta gatctgcgtg ctatggagtt tgagcacact aaagccttcg agccaatggt
     3121 gaccgagctc ttgaaacagc atttcggagc cctggccact gcagaagttg aggacaaagt
     3181 acgaaaatcc atctacaagc gtttgcagca gaacaacagt tacgacctgg aacagcggtg
     3241 gcatgataca ttctctgtcg ctacgggcgc agtggtagaa gccttggcag gacaggaggt
     3301 gtctttggca tccctgaacg cgtggaaggt agcatgtgcg gaaaaggcta ttgctttaac
     3361 acgctccgtg cgagacagct tttgggctgc cccaagctcc tcttcccccg ctctgaaata
     3421 cttgagccca cggacccgag tgttgtatag ctttgttcgt gaggaggtag gagtaaaggc
     3481 acgccgcggg gacgtgtatt tggggaaaca agaagtaact attgggacaa atgtatctcg
     3541 tatttatgaa gcgatcaaat ctggtcggat tgcccctgta ttagtcaaga tgatggccta
     3601 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatacgagag agagaatata
     3661 aaaagccaga ttattaatcc ggctttttta ttattttact agagaagctt ggctgttttg
     3721 gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga
     3781 taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact
     3841 cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga
     3901 actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     3961 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac
     4021 gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat
     4081 caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg
     4141 tttatttttc taaatacatt caaatatgta tccgctccta ggggatccca tggtacgcta
     4201 gctgaaagcg accaggtgct cggcgtggca agactcgcag cgaacccgta gaaagccatg
     4261 ctccagccgc ccgcattgga gaaattcttc aaattcccgt tgcacatagc ccggcaattc
     4321 ctttccctgc tctgccataa gcgcagcgaa tgccgggtaa tactcgtcaa cgatctgata
     4381 gagaagggtt tgctcgggtc ggtggctctg gtaacgacca gtatcccgat cccggctggc
     4441 cgtcctggcc gccacatgag gcatgttccg cgtccttgca atactgtgtt tacatacagt
     4501 ctatcgctta gcggaaagtt cttttaccct cagccgaaat gcctgccgtt gctagacatt
     4561 gccagccagt gcccgtcact cccgtactaa ctgtcacgaa cccctgcaat aactgtcacg
     4621 cccccctgca ataactgtca cgaacccctg caataactgt cacgccccca aacctgcaaa
     4681 cccagcaggg gcgggggctg gcggggtgtt ggaaaaatcc atccatgatt atctaagaat
     4741 aatccactag gcgcggttat cagcgccctt gtggggcgct gctgcccttg cccaatatgc
     4801 ccggccagag gccggatagc tggtctattc gctgcgctag gctacacacc gccccaccgc
     4861 tgcgcggcag ggggaaaggc gggcaaagcc cgctaaaccc cacaccaaac cccgcagaaa
     4921 tacgctggag cgcttttagc cgctttagcg gcctttcccc ctacccgaag ggtgggggcg
     4981 cgtgtgcagc cccgcagggc ctgtctcggt cgatcattca gcccggctca tccttctggc
     5041 gtggcggcag accgaacaag gcgcggtcgt ggtcgcgttc aaggtacgca tccattgccg
     5101 ccatgagccg atcctccggc cactcgctgc tgttcacctt ggccaaaatc atggccccca
     5161 ccagcacctt gcgccttgtt tcgttcttgc gctcttgctg ctgttccctt gcccgcaccc
     5221 gctgaatttc ggcattgatt cgcgctcgtt gttcttcgag cttggccagc cgatccgccg
     5281 ccttgttgct ccccttaacc atcttgacac cccattgtta atgtgctgtc tcgtaggcta
     5341 tcatggaggc acagcggcgg caatcccgac cctactttgt aggggagggc gcacttaccg
     5401 gtttctcttc gagaaactgg cctaacggcc acccttcggg cggtgcgctc tccgagggcc
     5461 attgcatgga gccgaaaagc aaaagcaaca gcgaggcagc atggcgattt atcaccttac
     5521 ggcgaaaacc ggcagcaggt cgggcggcca atcggccagg gccaaggccg actttatcca
     5581 gcgcgaaggc aagtatgccc gcgacatgga tgaagtcttg cacgccgaat ccgggcacat
     5641 gccggagttc gtcgagcggc ccgccgacta ctgggatgct gccgacctgt atgaacgcgc
     5701 caatgggcgg ctgttcaagg aggtcgaatt tgccctgccg gtcgagctga ccctcgacca
     5761 gcagaaggcg ctggcgtccg agttcgccca gcacctgacc ggtgccgagc gcctgccgta
     5821 tacgctggcc atccatgccg gtggcggcga gaacccgcac tgccacctga tgatctccga
     5881 gcggatcaat gacggcatcg agcggcccgc cgctcagtgg ttcaagcggt acaacggcaa
     5941 gaccccggag aagggcgggg cacagaagac cgaagcgctc aagcccaagg catggcttga
     6001 gcagacccgc gaggcatggg ccgaccatgc caaccgggca ttagagcggg ctggccacga
     6061 cgcccgcatt gaccacagaa cacttgaggc gcagggcatc gagcgcctgc ccggtgttca
     6121 cctggggccg aacgtggtgg agatggaagg ccggggcatc cgcaccgacc gggcagacgt
     6181 ggccctgaac atcgacaccg ccaacgccca gatcatcgac ttacaggaat accgggaggc
     6241 aatagaccat gaacgcaatc gacagagtga agaaatccag aggcatcaac gagttagcgg
     6301 agcagatcga accgctggcc cagagcatgg cgacactggc cgacgaagcc cggcaggtca
     6361 tgagccagac ccagcaggcc agcgaggcgc aggcggcgga gtggctgaaa gcccagcgcc
     6421 agacaggggc ggcatgggtg gagctggcca aagagttgcg ggaggtagcc gccgaggtga
     6481 gcagcgccgc gcagagcgcc cggagcgcgt cgcgggggtg gcactggaag ctatggctaa
     6541 ccgtgatgct ggcttccatg atgcctacgg tggtgctgct gatcgcatcg ttgctcttgc
     6601 tcgacctgac gccactgaca accgaggacg gctcgatctg gctgcgcttg gtggcccgat
     6661 gaagaacgac aggactttgc aggccatagg ccgacagctc aaggccatgg gctgtgagcg
     6721 cttcgatatc ggcgtcaggg acgccaccac cggccagatg atgaaccggg aatggtcagc
     6781 cgccgaagtg ctccagaaca cgccatggct caagcggatg aatgcccagg gcaatgacgt
     6841 gtatatcagg cccgccgagc aggagcggca tggtctggtg ctggtggacg acctcagcga
     6901 gtttgacctg gatgacatga aagccgaggg ccgggagcct gccctggtag tggaaaccag
     6961 cccgaagaac tatcaggcat gggtcaaggt ggccgacgcc gcaggcggtg aacttcgggg
     7021 gcagattgcc cggacgctgg ccagcgagta cgacgccgac ccggccagcg ccgacagccg
     7081 ccactatggc cgcttggcgg gcttcaccaa ccgcaaggac aagcacacca cccgcgccgg
     7141 ttatcagccg tgggtgctgc tgcgtgaatc caagggcaag accgccaccg ctggcccggc
     7201 gctggtgcag caggctggcc agcagatcga gcaggcccag cggcagcagg agaaggcccg
     7261 caggctggcc agcctcgaac tgcccgagcg gcagcttagc cgccaccggc gcacggcgct
     7321 ggacgagtac cgcagcgaga tggccgggct ggtcaagcgc ttcggtgatg acctcagcaa
     7381 gtgcgacttt atcgccgcgc agaagctggc cagccggggc cgcagtgccg aggaaatcgg
     7441 caaggccatg gccgaggcca gcccagcgct ggcagagcgc aagcccggcc acgaagcgga
     7501 ttacatcgag cgcaccgtca gcaaggtcat gggtctgccc agcgtccagc ttgcgcgggc
     7561 cgagctggca cgggcaccgg caccccgcca gcgaggcatg gacaggggcg ggccagattt
     7621 cagcatgtag tgcttgcgtt ggtactcacg cctgttatac tatgagtact cacgcacaga
     7681 agggggtttt atggaatacg aaaaaagcgc ttcagggtcg gtctacctga tcaaaagtga
     7741 caagggctat tggttgcccg gtggctttgg ttatacgtca aacaaggccg aggctggccg
     7801 cttttcagtc gctgatatgg ccagccttaa ccttgacggc tgcaccttgt ccttgttccg
     7861 cgaagacaag cctttcggcc ccggcaagtt tctcggtgac tgatatgaaa gaccaaaagg
     7921 acaagcagac cggcgacctg ctggccagcc ctgacgctgt acgccaagcg cgatatgccg
     7981 agcgcatgaa ggccaaaggg atgcgtcagc gcaagttctg gctgaccgac gacgaatacg
     8041 aggcgctgcg cgagtgcctg gaagaactca gagcggcgca gggcgggggt agtgaccccg
     8101 ccagcgccta accaccaact gcctgcaaag gaggcaatca atggctaccc ataagcctat
     8161 caatattctg gaggcgttcg cagcagcgcc gccaccgctg gactacgttt tgcccaacat
     8221 ggtggccggt acggtcgggg cgctggtgtc gcccggtggt gccggtaaat ccatgctggc
     8281 cctgcaactg gccgcacaga ttgcaggcgg gccggatctg ctggaggtgg gcgaactgcc
     8341 caccggcccg gtgatctacc tgcccgccga agacccgccc accgccattc atcaccgcct
     8401 gcacgccctt ggggcgcacc tcagcgccga ggaacggcaa gccgtggctg acggcctgct
     8461 gatccagccg ctgatcggca gcctgcccaa catcatggcc ccggagtggt tcgacggcct
     8521 caagcgcgcc gccgagggcc gccgcctgat ggtgctggac acgctgcgcc ggttccacat
     8581 cgaggaagaa aacgccagcg gccccatggc ccaggtcatc ggtcgcatgg aggccatcgc
     8641 cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc agcaagggcg cggccatgat
     8701 gggcgcaggc gaccagcagc aggccagccg gggcagctcg gtactggtcg ataacatccg
     8761 ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc gaggaatggg gtgtggacga
     8821 cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag gccaactatg gcgcaccgtt
     8881 cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc aagcccgccg tgctggagag
     8941 gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa gaacaagcac agcctcagcc
     9001 acgtccggca cgacccggcg cactgtctgg cccccggcct gttccgtgcc ctcaagcggg
     9061 gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta cggcgacggc aagcggatcg
     9121 agttcagcgg cccggagccg ctgggcgctg atgatctgcg catcctgcaa gggctggtgg
     9181 ccatggctgg gcctaatggc ctagtgcttg gcccggaacc caagaccgaa ggcggacggc
     9241 agctccggct gttcctggaa cccaagtggg aggccgtcac cgctgatgcc atggtggtca
     9301 aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga ggtcgatagt ggtggggcgc
     9361 tcaagcacat acaggactgc atcgagcgcc tttggaaggt atccatcatc gcccagaatg
     9421 gccgcaagcg gcaggggttt cggctgctgt cggagtacgc cagcgacgag gcggacgggc
     9481 gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt catgggtggc ggccagcatg
     9541 tgcgcatcag catggacgag gtgcgggcgc tggacagcga aaccgcccgc ctgctgcacc
     9601 agcggctgtg tggctggatc gaccccggca aaaccggcaa ggcttccata gataccttgt
     9661 gcggctatgt ctggccgtca gaggccagtg gttcgaccat gcgcaagcgc cgccagcggg
     9721 tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac ggtaaccgag ttcgcggcgg
     9781 gcaagtacga catcacccgg cccaaggcgg caggctgacc ccccccactc tattgtaaac
     9841 aagacatttt tatcttttat attcaatggc ttattttcct gctaattggt aataccatga
     9901 aaaataccat gctcagaaaa ggcttaacaa tattttgaaa aattgcctac tgagcgctgc
     9961 cgcacagctc cataggccgc tttcctggct ttgcttccag atgtatgctc ttctgctcct
    10021 gccggccgac tgtccctagt gcttggattc tcaccaataa aaaacgcccg gcggcaaccg
    10081 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
    10141 ccaagcgagc tcgatatccg tcggcttgaa cgaattgtta gacattattt gccgactacc
    10201 ttggtgatct cgcctttcac gtagtggaca aattcttcca actgatctgc gcgcgaggcc
    10261 aagcgatctt cttcttgtcc aagataagcc tgtctagctt caagtatgac gggctgatac
    10321 tgggccggca ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc gattttgccg
    10381 gttactgcgc tgtaccaaat gcgggacaac gtaagcacta catttcgctc atcgccagcc
    10441 cagtcgggcg gcgagttcca tagcgttaag gtttcattta gcgcctcaaa tagatcctgt
    10501 tcaggaaccg gatcaaagag ttcctccgcc gctggaccta ccaaggcaac gctatgttct
    10561 cttgcttttg tcagcaagat agccagatca atgtcgatcg tggctggctc gaagatacct
    10621 gcaagaatgt cattgcgctg ccattctcca aattgcagtt cgcgcttagc tggataacgc
    10681 cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag aatctcgctc
    10741 tctccagggg aagccgaagt ttccaaaagg tcgttgatca aagctcgccg cgttgtttca
    10801 tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt caggccgcca
    10861 tccactgcgg agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg
    10921 acgccaacta cctctgatag ttgagtcgat acttcggcga tcaccgcttc cctcatgatg
    10981 tttaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc
    11041 aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtacc
    11101 cc
//
