LOCUS       sequence-464943-       11114 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rhamnose inducible expression of Nicotiana tabacum PAL 4 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11114)
  AUTHORS   Hunstiger D, Ma H, Paton AJ, Peebles CAM
  TITLE     Improving trans-cinnamic acid production in a model cyanobacterium
  JOURNAL   Biotechnol. Prog. 2024;e3512.
REFERENCE   2  (bases 1 to 11114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Prog. 2024;e3512."
COMMENT     Sequence Label: pDF1b-iNTP
FEATURES             Location/Qualifiers
     source          1..11114
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      204..298
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(527..1378)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(1365..2204)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(2715..3686)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            4925..5012
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      5354..5748
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(6227..6254)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6346..6432)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     6585..6602
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      complement(6709..6736)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             8201..8215
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
     protein_bind    9067..9083
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    9100..9116
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             9160..9996
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
     promoter        complement(9995..10072)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     primer_bind     complement(10142..10164)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             join(10483..11114,1..160)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta
       61 tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca
      121 ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag
      181 ccgacggata tcgagctcgc ttggactcct gttgatagat ccagtaatga cctcagaact
      241 ccatctggat ttgttcagaa cgctcggttg ccgccgggcg ttttttattg gtgagaatcc
      301 aagcactagg gacagtcggc cggcaggagc agaagagcat acatctggaa gcaaagccag
      361 gaaagcggcc tatggagctg tgcggcagcg ctcagtaggc aatttttcaa aatattgtta
      421 agccttttct gagcatggta tttttcatgg tattaccaat tagcaggaaa ataagccatt
      481 gaatataaaa gataaaaatg tcttgtttac aatagagtgg ggggggtcag cctgccgcct
      541 tgggccgggt gatgtcgtac ttgcccgccg cgaactcggt taccgtccag cccagcgcga
      601 ccagctccgg caacgcctcg cgcacccgct ggcggcgctt gcgcatggtc gaaccactgg
      661 cctctgacgg ccagacatag ccgcacaagg tatctatgga agccttgccg gttttgccgg
      721 ggtcgatcca gccacacagc cgctggtgca gcaggcgggc ggtttcgctg tccagcgccc
      781 gcacctcgtc catgctgatg cgcacatgct ggccgccacc catgacggcc tgcgcgatca
      841 aggggttcag ggccacgtac aggcgcccgt ccgcctcgtc gctggcgtac tccgacagca
      901 gccgaaaccc ctgccgcttg cggccattct gggcgatgat ggataccttc caaaggcgct
      961 cgatgcagtc ctgtatgtgc ttgagcgccc caccactatc gacctctgcc ccgatttcct
     1021 ttgccagcgc ccgatagcta cctttgacca ccatggcatc agcggtgacg gcctcccact
     1081 tgggttccag gaacagccgg agctgccgtc cgccttcggt cttgggttcc gggccaagca
     1141 ctaggccatt aggcccagcc atggccacca gcccttgcag gatgcgcaga tcatcagcgc
     1201 ccagcggctc cgggccgctg aactcgatcc gcttgccgtc gccgtagtca tacgtcacgt
     1261 ccagcttgct gcgcttgcgc tcgccccgct tgagggcacg gaacaggccg ggggccagac
     1321 agtgcgccgg gtcgtgccgg acgtggctga ggctgtgctt gttcttaggc ttcaccacgg
     1381 ggcaccccct tgctcttgcg ctgcctctcc agcacggcgg gcttgagcac cccgccgtca
     1441 tgccgcctga accaccgatc agcgaacggt gcgccatagt tggccttgct cacaccgaag
     1501 cggacgaaga accggcgctg gtcgtcgtcc acaccccatt cctcggcctc ggcgctggtc
     1561 atgctcgaca ggtaggactg ccagcggatg ttatcgacca gtaccgagct gccccggctg
     1621 gcctgctgct ggtcgcctgc gcccatcatg gccgcgccct tgctggcatg gtgcaggaac
     1681 acgatagagc acccggtatc ggcggcgatg gcctccatgc gaccgatgac ctgggccatg
     1741 gggccgctgg cgttttcttc ctcgatgtgg aaccggcgca gcgtgtccag caccatcagg
     1801 cggcggccct cggcggcgcg cttgaggccg tcgaaccact ccggggccat gatgttgggc
     1861 aggctgccga tcagcggctg gatcagcagg ccgtcagcca cggcttgccg ttcctcggcg
     1921 ctgaggtgcg ccccaagggc gtgcaggcgg tgatgaatgg cggtgggcgg gtcttcggcg
     1981 ggcaggtaga tcaccgggcc ggtgggcagt tcgcccacct ccagcagatc cggcccgcct
     2041 gcaatctgtg cggccagttg cagggccagc atggatttac cggcaccacc gggcgacacc
     2101 agcgccccga ccgtaccggc caccatgttg ggcaaaacgt agtccagcgg tggcggcgct
     2161 gctgcgaacg cctccagaat attgataggc ttatgggtag ccattgattg cctcctttgc
     2221 aggcagttgg tggttaggcg ctggcggggt cactaccccc gccctgcgcc gctctgagtt
     2281 cttccaggca ctcgcgcagc gcctcgtatt cgtcgtcggt cagccagaac ttgcgctgac
     2341 gcatcccttt ggccttcatg cgctcggcat atcgcgcttg gcgtacagcg tcagggctgg
     2401 ccagcaggtc gccggtctgc ttgtcctttt ggtctttcat atcagtcacc gagaaacttg
     2461 ccggggccga aaggcttgtc ttcgcggaac aaggacaagg tgcagccgtc aaggttaagg
     2521 ctggccatat cagcgactga aaagcggcca gcctcggcct tgtttgacgt ataaccaaag
     2581 ccaccgggca accaatagcc cttgtcactt ttgatcaggt agaccgaccc tgaagcgctt
     2641 ttttcgtatt ccataaaacc cccttctgtg cgtgagtact catagtataa caggcgtgag
     2701 taccaacgca agcactacat gctgaaatct ggcccgcccc tgtccatgcc tcgctggcgg
     2761 ggtgccggtg cccgtgccag ctcggcccgc gcaagctgga cgctgggcag acccatgacc
     2821 ttgctgacgg tgcgctcgat gtaatccgct tcgtggccgg gcttgcgctc tgccagcgct
     2881 gggctggcct cggccatggc cttgccgatt tcctcggcac tgcggccccg gctggccagc
     2941 ttctgcgcgg cgataaagtc gcacttgctg aggtcatcac cgaagcgctt gaccagcccg
     3001 gccatctcgc tgcggtactc gtccagcgcc gtgcgccggt ggcggctaag ctgccgctcg
     3061 ggcagttcga ggctggccag cctgcgggcc ttctcctgct gccgctgggc ctgctcgatc
     3121 tgctggccag cctgctgcac cagcgccggg ccagcggtgg cggtcttgcc cttggattca
     3181 cgcagcagca cccacggctg ataaccggcg cgggtggtgt gcttgtcctt gcggttggtg
     3241 aagcccgcca agcggccata gtggcggctg tcggcgctgg ccgggtcggc gtcgtactcg
     3301 ctggccagcg tccgggcaat ctgcccccga agttcaccgc ctgcggcgtc ggccaccttg
     3361 acccatgcct gatagttctt cgggctggtt tccactacca gggcaggctc ccggccctcg
     3421 gctttcatgt catccaggtc aaactcgctg aggtcgtcca ccagcaccag accatgccgc
     3481 tcctgctcgg cgggcctgat atacacgtca ttgccctggg cattcatccg cttgagccat
     3541 ggcgtgttct ggagcacttc ggcggctgac cattcccggt tcatcatctg gccggtggtg
     3601 gcgtccctga cgccgatatc gaagcgctca cagcccatgg ccttgagctg tcggcctatg
     3661 gcctgcaaag tcctgtcgtt cttcatcggg ccaccaagcg cagccagatc gagccgtcct
     3721 cggttgtcag tggcgtcagg tcgagcaaga gcaacgatgc gatcagcagc accaccgtag
     3781 gcatcatgga agccagcatc acggttagcc atagcttcca gtgccacccc cgcgacgcgc
     3841 tccgggcgct ctgcgcggcg ctgctcacct cggcggctac ctcccgcaac tctttggcca
     3901 gctccaccca tgccgcccct gtctggcgct gggctttcag ccactccgcc gcctgcgcct
     3961 cgctggcctg ctgggtctgg ctcatgacct gccgggcttc gtcggccagt gtcgccatgc
     4021 tctgggccag cggttcgatc tgctccgcta actcgttgat gcctctggat ttcttcactc
     4081 tgtcgattgc gttcatggtc tattgcctcc cggtattcct gtaagtcgat gatctgggcg
     4141 ttggcggtgt cgatgttcag ggccacgtct gcccggtcgg tgcggatgcc ccggccttcc
     4201 atctccacca cgttcggccc caggtgaaca ccgggcaggc gctcgatgcc ctgcgcctca
     4261 agtgttctgt ggtcaatgcg ggcgtcgtgg ccagcccgct ctaatgcccg gttggcatgg
     4321 tcggcccatg cctcgcgggt ctgctcaagc catgccttgg gcttgagcgc ttcggtcttc
     4381 tgtgccccgc ccttctccgg ggtcttgccg ttgtaccgct tgaaccactg agcggcgggc
     4441 cgctcgatgc cgtcattgat ccgctcggag atcatcaggt ggcagtgcgg gttctcgccg
     4501 ccaccggcat ggatggccag cgtatacggc aggcgctcgg caccggtcag gtgctgggcg
     4561 aactcggacg ccagcgcctt ctgctggtcg agggtcagct cgaccggcag ggcaaattcg
     4621 acctccttga acagccgccc attggcgcgt tcatacaggt cggcagcatc ccagtagtcg
     4681 gcgggccgct cgacgaactc cggcatgtgc ccggattcgg cgtgcaagac ttcatccatg
     4741 tcgcgggcat acttgccttc gcgctggata aagtcggcct tggccctggc cgattggccg
     4801 cccgacctgc tgccggtttt cgccgtaagg tgataaatcg ccatgctgcc tcgctgttgc
     4861 ttttgctttt cggctccatg caatggccct cggagagcgc accgcccgaa gggtggccgt
     4921 taggccagtt tctcgaagag aaaccggtaa gtgcgccctc ccctacaaag tagggtcggg
     4981 attgccgccg ctgtgcctcc atgatagcct acgagacagc acattaacaa tggggtgtca
     5041 agatggttaa ggggagcaac aaggcggcgg atcggctggc caagctcgaa gaacaacgag
     5101 cgcgaatcaa tgccgaaatt cagcgggtgc gggcaaggga acagcagcaa gagcgcaaga
     5161 acgaaacaag gcgcaaggtg ctggtggggg ccatgatttt ggccaaggtg aacagcagcg
     5221 agtggccgga ggatcggctc atggcggcaa tggatgcgta ccttgaacgc gaccacgacc
     5281 gcgccttgtt cggtctgccg ccacgccaga aggatgagcc gggctgaatg atcgaccgag
     5341 acaggccctg cggggctgca cacgcgcccc cacccttcgg gtagggggaa aggccgctaa
     5401 agcggctaaa agcgctccag cgtatttctg cggggtttgg tgtggggttt agcgggcttt
     5461 gcccgccttt ccccctgccg cgcagcggtg gggcggtgtg tagcctagcg cagcgaatag
     5521 accagctatc cggcctctgg ccgggcatat tgggcaaggg cagcagcgcc ccacaagggc
     5581 gctgataacc gcgcctagtg gattattctt agataatcat ggatggattt ttccaacacc
     5641 ccgccagccc ccgcccctgc tgggtttgca ggtttggggg cgtgacagtt attgcagggg
     5701 ttcgtgacag ttattgcagg ggggcgtgac agttattgca ggggttcgtg acagttagta
     5761 cgggagtgac gggcactggc tggcaatgtc tagcaacggc aggcatttcg gctgagggta
     5821 aaagaacttt ccgctaagcg atagactgta tgtaaacaca gtattgcaag gacgcggaac
     5881 atgcctcatg tggcggccag gacggccagc cgggatcggg atactggtcg ttaccagagc
     5941 caccgacccg agcaaaccct tctctatcag atcgttgacg agtattaccc ggcattcgct
     6001 gcgcttatgg cagagcaggg aaaggaattg ccgggctatg tgcaacggga atttgaagaa
     6061 tttctccaat gcgggcggct ggagcatggc tttctacggg ttcgctgcga gtcttgccac
     6121 gccgagcacc tggtcgcttt cagctagcgt accatgggat cccctaggag cggatacata
     6181 tttgaatgta tttagaaaaa taaacaaaag agtttgtaga aacgcaaaaa ggccatccgt
     6241 caggatggcc ttctgcttaa tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
     6301 ccctccgggc cgttgcttcg caacgttcaa atccgctccc ggcggatttg tcctactcag
     6361 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt
     6421 tcgttttatt tgatgcctgg cagttcccta ctctcgcatg gggagacccc acactaccat
     6481 cggcgctacg gcgtttcact tctgagttcg gcatggggtc aggtgggacc accgcgctac
     6541 tgccgccagg caaattctgt tttatcagac cgcttctgcg ttctgattta atctgtatca
     6601 ggctgaaaat cttctctcat ccgccaaaac agccaagctt ctctagtaaa ataataaaaa
     6661 agccggatta ataatctggc tttttatatt ctctctctcg tatataaacg cagaaaggcc
     6721 cacccgaagg tgagccagtg tgattaacag atcggtagag gcgcaccatt ccagcttttg
     6781 agacactcta acattggatc aataatctgt ccattgcaca tcgctgtgaa cactttctca
     6841 cactcctcgc ccggtgatcg gacacgttca cctgtcagta gttccgttcc cagctcttta
     6901 cgcacaaagc gatacagtgg ataggaacgg cattcggtta tgcggttggc tatcgcaggg
     6961 ttgccacatt ccaaggcagc gcgtgcgctc tcaacttctt ttggtaatac ggcctttaat
     7021 tcgtcttcga aggctccaat tttttggaag attgagctgt tcacattttt ctcgctttcc
     7081 ccattattca tcgcatgatc cacaagtacc tgacgcagtt tctgcattaa cgggtagttg
     7141 cacgagcaag gatcatcagc ataggcgaac aagtactcgc ggtccacgac gcgcagcagt
     7201 tccttttcac agaagcgtgc cggatgcagt tcaccgttcg cacccatcgt aagggtccgc
     7261 ttagctactt ggctcacagt gttcttgact gcatttttca ggttttcctc caaatgacgc
     7321 aggtcgatcg cctggcaaag tgccaccaga taggttgagc tcatgagttt taagatatcg
     7381 accgcttccg cggttttacg tgccgagatc aggcccaagg agttgacatc ctggttgtgt
     7441 tgctcggccg actggacatg gttcgtcacc ggattggcca aaaactgaag ttctgagcag
     7501 taagatgcca tggcgatttc ggcccccttg aaaccatagt ccaggctggg attgcgtgat
     7561 gcggtcaggt tagacggcag gccgttgttg taatagtcgt tgaccagttc cgaaaattga
     7621 gcaaacatca gtttcccaat tgatgcaaga gccagacgtg cattatccat ggacacaccg
     7681 atcggggtgc cttggaagtt gccaccgtgt aatgctttgt ttctgctaac atcgatcagg
     7741 gggttatcat tcaccgagtt aatctcgcgc tctatcattt tcgttgccgc gcgaatgact
     7801 tcgatttgcg ggcccagcca ctgtggagag gttcgcagag cataacgatc ctgctttggt
     7861 ttctggagcg gatccatttc atgtagcttc tgcgccgcct tcacataaga gctgccgtcc
     7921 agaatatgtt ccataatggc agccgcctca atttgccccg ggtggtgctt caatttgtgc
     7981 gtcagatggt cagtaaattc gggttttccg ttcattactt cagcgaaaat ggcgcttaaa
     8041 acttcagaca taacggccag aatgttggaa tcaaagagga ccatggacgc catgccagaa
     8101 ccaactgcgg tgccattcac cagcgccagt ccttctttcg gctgcagctc gaaaaaccca
     8161 ccgttaacac ccgccacgcg gaacgcttct tccgcattga gggtctcacc attgggacta
     8221 actgctttgg aattagggcg accggtgagc aaaccagcaa tgtaggataa aggaaccagg
     8281 tcgcccgagg ccgtgatcgt gccacgcagc ggtaaacacg gagtaatgtt gctgttaatc
     8341 aatttggcaa ttgcttccaa gatttcaaaa cggatgccgg agtagccctg caggagtgtg
     8401 ttgatacgaa caagcatagc ggcgcgtgtc gcactgtgtg gcagcgtgtg gcttgtttcc
     8461 gtgccattgc caaaaacccc cgcattcaaa aacctaatca gttctttctg cagggcacca
     8521 ccattcttgg tacggcgatg cgaggtagcg ccaaacccag tcgtaacacc ataactatcg
     8581 gtccctttat tcatactgtc cattacccaa tcactactcg ctttgacgcc cgcccgggcc
     8641 tcttcgctaa gttctacttt tacgccattc gcacttttgt cccgaaccgc aatggccgcc
     8701 acctgcgcca cggttaaagt ttcgccacca agttttacca tcggtttccg aaattcactc
     8761 accatttttt tcacttcatc cagatgactc cctctcagag attcagctgc catttcccaa
     8821 ttcagcggat cagtggctga cgattttttg cacagctcaa agttttcgcc tccattcaca
     8881 tgaccattgc ttgccatttc tacctccttt gtatattata aacttacccg attcattacg
     8941 accagtctaa aaagcgcctg aatacgcgac cttctcgtta ctgacaggaa aatgggccat
     9001 tggcaaccag ggaaagatga acgtgatgat gttcacaatt tgctgaattg tggaaagagt
     9061 gttgacttgt gagcggataa caatgatact tagattcaat tgtgagcgga taacaatttc
     9121 acacatattt cgccgtgttg acgacatcag gaggccagta tgaccgtatt acatagtgtg
     9181 gatttttttc cgtctggtaa cgcgtccgtg gcgatagaac cccggctccc gcaggcggat
     9241 tttcctgaac atcatcatga ttttcatgaa attgtgattg tcgaacatgg cacgggtatt
     9301 catgtgttta atgggcagcc ctataccatc accggtggca cggtctgttt cgtacgcgat
     9361 catgatcggc atctgtatga acataccgat aatctgtgtc tgaccaatgt gctgtatcgc
     9421 tcgccggatc gatttcagtt tctcgccggg ctgaatcagt tgctgccaca agagctggat
     9481 gggcagtatc cgtcccactg gcgcgttaac cacagcgtat tgcagcaggt tcgacagctg
     9541 gttgcacaga tggaacagca ggaaggggaa aatgatttac cctcgaccgc cagtcgcgag
     9601 atcttgttta tgcaattact gctcttgctg cgtaaaagca gtttgcagga gaacctggaa
     9661 aacagcgcat cacgcctcaa cttgcttctg gcctggctgg aggaccattt tgccgatgag
     9721 gtgaattggg atgccgtggc ggatcaattt tctctttcac tgcgtacgct acatcggcag
     9781 cttaagcagc aaacgggact gacgcctcag cgatacctga accgcctgcg actgatgaaa
     9841 gcccgacatc tgctacgcca cagcgaggcc agcgttactg acatcgccta tcgctgtgga
     9901 ttcagcgaca gtaaccactt ttcgacgctt tttcgccgag agtttaactg gtcaccgcgt
     9961 gatattcgcc agggacggga tggctttctg caataatcac caccctgaat tgactctctt
    10021 ccgggcgcta tcatgccata ccgcgaaagg ttttgcacca ttcgatggtg tcaacgtgcc
    10081 gcttcgcctt cgcgcgcgaa ttgatctgct gcctcgcgcg tttcggtgat gacggtgaaa
    10141 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
    10201 gtgtacacac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag
    10261 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
    10321 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca
    10381 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
    10441 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc
    10501 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
    10561 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
    10621 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
    10681 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
    10741 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
    10801 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
    10861 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
    10921 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
    10981 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
    11041 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
    11101 gtcgctgccg actg
//
