LOCUS       sequence-464945-       10679 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ncHLAp-reactive T cell receptor (TCR004H).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10679)
  AUTHORS   Ely ZA, Kulstad ZJ, Gunaydin G, Addepalli S, Verzani EK, Casarrubios
            M, Clauser KR, Wang X, Lippincott IE, Louvet C, Schmitt T, Kapner 
            KS, Agus MP, Hennessey CJ, Cleary JM, Hadrup SR, Klaeger S, Su J, 
            Jaeger AM, Wolpin BM, Raghavan S, Smith EL, Greenberg PD, Aguirre 
            AJ, Abelin JG, Carr SA, Jacks T, Freed-Pastor WA.
  TITLE     Pancreatic cancer-restricted cryptic antigens are targets for T cell
            recognition.
  JOURNAL   Science. 2025. May 8; 388(6747)
REFERENCE   2  (bases 1 to 10679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2025. May 8; 388(6747)"
COMMENT     Sequence Label: TCR004H
FEATURES             Location/Qualifiers
     source          1..10679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2638..3816
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2869..3807
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3764..3784
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3847..3856
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4804..4860
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    5771..6359
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5824..5844)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6242..6253)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6431..6664
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6690..6707)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6696..6920
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6966..7394
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7053..7072)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7263..7284
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7403..7423)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7408..7737
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7588..7723
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7650..7669
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7785..7832
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7851..8225
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8355..8476
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8392..8411)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8446..8465
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8525..8541)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8525..8541)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8538..8560)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8549..8565
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8573..8579,8580..8597,8598..8603))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8618..8639
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8756..8773)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8927..9515)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9007..9026)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9686..10477,10478..10546))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10309..10328
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10547..10651)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aggtaccgat cagtgtgagg gagtgtaaag ctggtttggc
     2641 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
     2701 ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
     2761 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
     2821 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg
     2881 tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact
     2941 tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag
     3001 agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct
     3061 gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc
     3121 gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa
     3181 gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg
     3241 ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg
     3301 cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc
     3361 ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt
     3421 gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg
     3481 cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc
     3541 tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag
     3601 ttctagagct tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag
     3661 tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc
     3721 tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg
     3781 gttcaaagtt tttttcttcc atttcaggtg tcgtgactaa ctcgaacgct agctgtgcga
     3841 tcgtttgcca ccatgggcac caggctcctc tgctgggtgg tcctgggttt cctagggaca
     3901 gatcacacag gtgctggagt ctcccagtcc cctaggtaca aagtcgcaaa gagaggacag
     3961 gatgtagctc tcaggtgtga tccaatttcg ggtcatgtat cccttttttg gtaccaacag
     4021 gccctggggc aggggccaga gtttctgact tatttccaga atgaagctca actagacaaa
     4081 tcggggctgc ccagtgatcg cttctttgca gaaaggcctg agggatccgt ctccactctg
     4141 aagatccagc gcacacagca ggaggactcc gccgtgtatc tctgtgccag cagcccggag
     4201 gcagggggtg aaaaactgtt ttttggcagt ggaacccagc tctctgtctt ggaggacctg
     4261 aacaaggtgt tcccacccga ggtcgctgtg tttgagccat cagaagcaga gatctcccac
     4321 acccaaaagg ccacactggt gtgcctggcc acaggcttct tccctgacca cgtggagctg
     4381 agctggtggg tgaatggcaa agaagtgcat tctggggtct gcacggaccc gcagcccctc
     4441 aaggagcagc ccgccctcaa tgactccaga tactgcctga gcagccgcct gagggtctcg
     4501 gccaccttct ggcagaaccc ccgcaaccac ttccgctgtc aagtccagtt ctacgggctc
     4561 tcggagaatg acgagtggac ccaggatagg gccaaacccg tcacccagat cgtcagcgcc
     4621 gaggcctggg gtagagcaga ctgtggcttt acctcggtgt cctaccagca aggggtcctg
     4681 tctgccacca tcctctatga gatcctgcta gggaaggcca ccctgtatgc tgtgctggtc
     4741 agcgcccttg tgttgatggc catggtcaag agaaaggatt tcagagccaa aagaggaagc
     4801 ggcgccacaa acttctcact gctgaaacag gccggcgacg tggaagagaa tcccggacct
     4861 atggcaggca ttcgagcttt atttatgtac ttgtggctgc agctggactg ggtgagcaga
     4921 ggagagagtg tggggctgca tcttcctacc ctgagtgtcc aggagggtga caactctatt
     4981 atcaactgtg cttattcaaa cagcgcctca gactacttca tttggtacaa gcaagaatct
     5041 ggaaaaggtc ctcaattcat tatagacatt cgttcaaata tggacaaaag gcaaggccaa
     5101 agagtcaccg ttttattgaa taagacagtg aaacatctct ctctgcaaat tgcagctact
     5161 caacctggag actcagctgt ctacttttgt gcagagaccc ccccttcagg aaacacacct
     5221 cttgtctttg gaaagggcac aagactttct gtgattgcaa atatccagaa ccctgaccct
     5281 gctgtgtatc agctgcgtga tagcaaatcc agtgacaagt ctgtctgcct attcaccgat
     5341 tttgattctc aaacaaatgt gtcacaaagt aaggattctg atgtgtatat cacagacaaa
     5401 tgcgtgctag acatgaggtc tatggacttc aagagcaaca gtgctgtggc ctggagcaac
     5461 aaatctgact ttgcatgtgc aaacgccttc aacaacagca ttattccaga agacaccttc
     5521 ttccccagcc cagaaagttc ctgtgatgtc aagctggtcg agaaaagctt tgaaacagat
     5581 acgaacctaa actttcaaaa cctgtcagtg attgggttcc gaatcctcct cctgaaagtg
     5641 gccgggttta atctgctcat gacgctgcgg ctgtggtcca gctgaaaacg ttgttgtttg
     5701 gggttgaatt actctacatg tttaagggtt ccggttccac taggtacaat tcgatatcaa
     5761 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     5821 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     5881 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     5941 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     6001 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     6061 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     6121 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     6181 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     6241 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     6301 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg
     6361 tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     6421 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     6481 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     6541 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     6601 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     6661 agcagggccc ggcgaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     6721 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     6781 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     6841 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     6901 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     6961 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     7021 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     7081 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     7141 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     7201 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     7261 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     7321 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     7381 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     7441 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     7501 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     7561 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     7621 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     7681 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     7741 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat
     7801 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt
     7861 tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga
     7921 ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg
     7981 acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg
     8041 cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca
     8101 cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc
     8161 gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg
     8221 actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     8281 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     8341 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     8401 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     8461 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     8521 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     8581 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     8641 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     8701 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     8761 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     8821 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     8881 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8941 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     9001 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     9061 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     9121 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     9181 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     9241 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     9301 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     9361 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     9421 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     9481 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     9541 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     9601 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9661 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9721 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     9781 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9841 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9901 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9961 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    10021 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    10081 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    10141 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    10201 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    10261 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    10321 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    10381 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    10441 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    10501 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    10561 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    10621 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgac
//
