LOCUS       sequence-464952-       12099 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express GFP from an EFS promoter in the 3'-5' direction and 
            Flag-GluN2B from a TRE3g promoter in the 5'-3' direction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12099)
  TITLE     Ploski Lab Plasmids 2025
REFERENCE   2  (bases 1 to 12099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti EFS-GFP-pa (opposite orientation)-
            TRE3...
FEATURES             Location/Qualifiers
     source          1..12099
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2859..2976
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(join(3078..3791,3792..3794,3795..3797))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(3081..3791,3792..3794,3795..3797))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3123..3144)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3472..3491
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3731..3752
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      3794..3803
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3827..4038)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     promoter        4092..4470
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    4100..4118
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4136..4154
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4172..4190
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4208..4226
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4244..4262
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4280..4298
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4316..4334
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4379..4403
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4645..4668
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    9007..9595
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9060..9080)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9478..9489)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9675..9908
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9980..10101
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     10071..10090
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10141..10276
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10296..10315)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10297..10315)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10325..10342)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10325..10341)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10334..10356)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10483..10938
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10570..10589)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10780..10801
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10964..11068
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11069..11137,11138..11929)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11287..11306)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt
     2881 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2941 gaattacaaa aacaaattac aaaaattcaa aattttatcg atgcggccgc tcggtccagt
     3001 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     3061 gctgcaataa actcgagtta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg
     3121 gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg
     3181 gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg
     3241 ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg
     3301 aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg
     3361 tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc
     3421 agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag
     3481 ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac
     3541 ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag
     3601 gtcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac
     3661 ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg
     3721 tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc
     3781 tcgcccttgc tcaccatggt ggcggcgaat tcccgcgtca cgacacctgt gttctggcgg
     3841 caaacccgtt gcgaaaaaga acgttcacgg cgactactgc acttatatac ggttctcccc
     3901 caccctcggg aaaaaggcgg agccagtaca cgacatcact ttcccagttt accccgcgcc
     3961 accttctcta ggcaccggtt caattgccga cccctccccc caacttctcg gggactgtgg
     4021 gcgatgtgcg ctctgcccac tgacgggcac cggagccact cgaacgcgta tcacgagact
     4081 agcctcgagt tggctttact ccctatcagt gatagagaac gtatgaagag tttactccct
     4141 atcagtgata gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg
     4201 agtttactcc ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga
     4261 gaacgtatct acagtttact ccctatcagt gatagagaac gtatatccag tttactccct
     4321 atcagtgata gagaacgtat aagctttagg cgtgtacggt gggcgcctat aaaagcagag
     4381 ctcgtttagt gaaccgtcag atcgcctgga gcaattccac aacacttttg tcttatacca
     4441 actttccgta ccacttccta ccctcgtaaa ggatccgccg ccaccatgaa gcccagcgca
     4501 gagtgctgtt ctcccaagtt ctggttggtg ttggccgtct tggccgtatc gggcagcaaa
     4561 gctcgttccc aaaagagcgc ccccagcatc ggcatcgctg tcatcctcgt gggcacttcc
     4621 gacgaagtgg ccataaaaga tgccgactac aaggacgacg atgacaagca cgagaaagat
     4681 gacttccatc atctctcagt agttccccgg gtggagctgg tagccatgaa cgagactgac
     4741 ccaaagagca taatcacccg catctgcgat cttatgtctg accggaagat ccagggggtg
     4801 gtgttcgcgg atgacacgga ccaggaagcc atcgcccaga tcctcgattt catttctgct
     4861 cagactctca cccccatcct gggcatccat gggggctcat ctatgataat ggcagataag
     4921 gatgagtcct ccatgttctt ccagtttggc ccatccattg aacagcaagc ttctgtcatg
     4981 ctcaacatca tggaagaata cgactggtac atcttctcca tcgtcaccac ctacttcccc
     5041 ggctaccagg acttcgtgaa caagatccgc agcactattg agaacagctt tgtaggctgg
     5101 gagctcgagg aagtcctcct gctagacatg tctctagacg atggcgactc taagattcag
     5161 aatcagctga agaagctgca gagccccatc attctcctct actgcacaaa ggaagaagcc
     5221 acctacatct tcgaagtagc taactcagtt gggctgactg gctacggcta cacatggatc
     5281 gtgccgagtc tggtggcggg ggatacagac acggtgcctt cagagttccc cacggggctc
     5341 atctctgtgt catatgacga atgggactat ggccttcctg ccagagtgag agatgggatt
     5401 gccatcatca ccactgctgc ctcggacatg ctgtccgaac acagtttcat ccctgagccc
     5461 aagagcagtt gctacaacac ccacgagaag aggatctacc agtctaacat gctgaatagg
     5521 tatctgatca acgtcacttt tgaagggaga aacctgtcct tcagtgaaga tggctaccag
     5581 atgcatccga agctggtgat aatccttctg aacaaggaga ggaagtggga gagggtggga
     5641 aaatggaaag acaagtccct gcagatgaaa tactacgtgt ggcctcgaat gtgtccagag
     5701 actgaagaac aggaagatga ccatctgagc atcgttacct tggaggaggc accgtttgtc
     5761 attgtggaaa gtgtggaccc tctcagtggg acctgcatgc ggaatacagt cccgtgccag
     5821 aagcgcatca tctctgagaa taaaacagat gaggaaccag gctacatcaa aaaatgctgc
     5881 aaggggtttt gtattgatat ccttaagaaa atttctaagt ctgtgaagtt cacctatgac
     5941 ctttacctgg tgaccaatgg caagcatgga aagaaaatca acgggacctg gaacggcatg
     6001 attggtgagg tggtcatgaa gagggcctac atggcagtgg gatcactaac tatcaatgaa
     6061 gaacggtcag aggtggttga cttctctgtg cccttcatag agactggcat cagtgtcatg
     6121 gtatcacgca gcaatgggac tgtgtcacct tctgccttct tagagccatt cagtgctgac
     6181 gtgtgggtga tgatgtttgt gatgctgctc attgtctctg ctgtagctgt ctttgtcttt
     6241 gaatacttca gccctgtggg ttacaaccgg tgcctagctg atggcagaga gccaggcggc
     6301 ccatctttca ccatcggcaa agcgatttgg ttactctggg gtctggtgtt taacaactcc
     6361 gtacctgtgc agaacccaaa ggggaccacc tccaagatca tggtgtcagt gtgggccttc
     6421 tttgctgtca ttttcctggc cagctacact gccaacttag ccgccttcat gatccaagag
     6481 gagtatgtgg accaggtttc cggcctgagt gacaagaagt tccagagacc taatgacttc
     6541 tcaccccctt tccgctttgg gactgtgccc aatggcagca cagagaggaa tatccgtaat
     6601 aactatgcag aaatgcatgc ctacatggga aagttcaacc aaaggggtgt agatgatgcc
     6661 ttgctctccc tgaaaacagg gaaacttgat gcattcatct acgatgcagc cgtgctcaac
     6721 tacatggctg gaagagacga aggctgcaag ctggtgacca ttggcagtgg caaggtcttt
     6781 gcttctacgg gctatggcat tgctatccaa aaagactctg gttggaaacg ccaggtggac
     6841 cttgctatcc tgcagctgtt tggagatggg gagatggaag aactggaagc tctctggctc
     6901 actggcattt gccacaatga gaagaatgag gttatgagca gccagctgga cattgacaac
     6961 atggcgggcg tcttctatat gttgggggca gccatggctc tcagcctcat caccttcatc
     7021 tgtgaacatc tcttctattg gcagttccga cattgcttca tgggtgtctg ttctggcaag
     7081 cctggcatgg tcttctccat cagcagaggt atctacagct gtatccacgg agtagctata
     7141 gaggagcgcc aatccgtgat gaactccccc actgccacca tgaacaacac acactccaat
     7201 atcctacgct tgctccgaac ggccaaaaac atggccaacc tgtctggagt caacggctcc
     7261 ccccagagtg ccctggactt catccgccgt gagtcctctg tctatgacat ctctgagcat
     7321 cgccgcagct tcacgcattc agactgcaag tcgtacaata acccaccctg tgaggaaaac
     7381 ctgttcagtg actacattag tgaggtagag agaacatttg gcaacctgca gctgaaggac
     7441 agcaatgtgt accaagacca ctatcaccat caccaccggc cccacagcat cggcagcacc
     7501 agctccattg atgggctcta tgactgtgac aacccaccct ttaccaccca gcccaggtca
     7561 atcagcaaga aacccctgga cattggcctg ccctcctcca aacacagcca gctcagcgac
     7621 ctgtacggca agttctcttt caagagtgac cgctacagtg gccatgatga cttgattcga
     7681 tcggatgtct cagacatctc cacgcatact gtcacctatg gcaacatcga gggcaacgca
     7741 gccaagagga ggaagcagca atataaggac agtctaaaga agcggccagc ctcggccaaa
     7801 tctaggaggg agtttgatga aatcgagctg gcctaccgtc gccgaccacc ccgctcccca
     7861 gaccacaagc gctacttcag ggacaaagaa gggctccgag acttctacct ggaccagttc
     7921 cgaacaaagg agaactcgcc tcactgggag cacgtggact taactgacat ttacaaagaa
     7981 cgtagtgatg acttcaagcg agattcggtc agtggaggcg ggccctgtac caacaggtct
     8041 caccttaaac acggaacagg cgataagcac ggagtggtag gcggggtgcc tgctccttgg
     8101 gagaagaacc tgaccaatgt ggattgggag gataggtctg ggggcaactt ctgccgcagc
     8161 tgtccctcca agctgcacaa ttactcctct acggtggcag ggcaaaactc gggccggcag
     8221 gcctgcatca ggtgtgaggc ctgcaagaag gctggcaacc tgtatgacat cagcgaggac
     8281 aactccctgc aggaactgga ccagcctgct gcccctgtgg ctgtgtcatc caacgcctcc
     8341 accaccaagt atcctcaaag cccgactaat tccaaggccc agaagaagaa tcggaacaaa
     8401 ctgcgccggc agcactccta cgacaccttc gtggacctgc agaaggagga ggccgccttg
     8461 gccccacgca gcgtgagcct gaaagacaag ggccgattca tggatgggag cccctacgcc
     8521 catatgtttg agatgccagc tggtgagagc tcctttgcca acaagtcctc agtgaccact
     8581 gccggacacc atcacaacaa tcccggcagc ggctacatgc tcagcaagtc gctctaccct
     8641 gaccgggtca cgcaaaaccc tttcatcccc acttttgggg atgatcagtg cttgcttcac
     8701 ggcagcaaat cctacttctt caggcagccc acggtggcag gggcgtcgaa aacaaggccg
     8761 gacttccggg cccttgtcac caataagcca gtggtgtcag cccttcatgg ggctgtgcca
     8821 ggtcgtttcc agaaggacat ttgtataggg aaccagtcca acccctgtgt gcctaacaac
     8881 aaaaacccca gggctttcaa tggctccagc aatggacatg tttatgagaa actttctagt
     8941 attcagtctg atgtctgagt gaggggaatt gatccggtac ccgcgtaccg gttagtaatg
     9001 atcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     9061 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     9121 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     9181 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     9241 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     9301 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     9361 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     9421 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     9481 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     9541 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggc
     9601 gatggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     9661 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     9721 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     9781 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     9841 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     9901 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     9961 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
    10021 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    10081 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
    10141 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
    10201 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
    10261 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
    10321 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
    10381 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
    10441 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
    10501 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
    10561 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
    10621 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
    10681 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
    10741 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
    10801 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
    10861 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
    10921 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
    10981 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
    11041 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
    11101 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
    11161 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
    11221 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
    11281 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
    11341 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
    11401 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
    11461 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
    11521 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
    11581 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
    11641 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
    11701 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
    11761 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
    11821 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
    11881 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
    11941 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
    12001 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
    12061 ctgagcgtca gaccccgtag aaaagatcaa aggatcttc
//
