LOCUS       sequence-464957-        9740 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BLADE (two-input, four-output decoder); Cre excises lox sites to 
            express GFP, Flp excises FRT sites to express iRFP, and the presence
            of both recombinases is designed to express mRuby..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9740)
  AUTHORS   Tous C, Kinstlinger IS, Rice MEL, Deng J, Wong WW
  TITLE     Multiplexing light-inducible recombinases to control cell fate, 
            Boolean logic, and cell patterning in mammalian cells.
  JOURNAL   Sci Adv. 2025 May 9;11(19):eadt1971. doi: 10.1126/sciadv.adt1971. 
            Epub 2025 May 9.
  PUBMED    40344052
REFERENCE   2  (bases 1 to 9740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-05-9"; volume: "11(19):eadt1971. doi"; pages: " 
            10.1126/sciadv.adt1971. Epub 2025 May 9"
COMMENT     Sequence Label: CT691
FEATURES             Location/Qualifiers
     source          1..9740
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(114..133)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      927..1515
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1416..1435
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1669..1686
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    1754..1888
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1778..1797)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1832..1851
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      1908..2042
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    2127..2148
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2163..2168,2169..2186,2187..2193)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2201..2217
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2228
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2225..2241
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2225..2241
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2584..2603
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    2602..2657
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     2703..2722
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2968..3192
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3196..3906)
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     regulatory      3903..3912
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    3922..3955
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    4076..4300
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(4301..5251)
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 720 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP720
                     /note="derived from Rhodopseudomonas palustris RpBphP2"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGSVIAEDRCAEVESKLGLHYPASFIPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPNHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
     regulatory      5248..5257
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    5267..5300
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(5302..5335)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    5456..5680
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(join(5681..6394,6395..6397,6398..6400))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(5684..6394,6395..6397,6398..6400))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5726..5747)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6075..6094
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6334..6355
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      6397..6406
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    6416..6449
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     polyA_signal    6570..6794
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(6795..7589)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6906..6925)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7516..7535
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     protein_bind    7632..7665
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(7667..7700)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(7799..7818)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          7826..8842
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(7880..7902)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     enhancer        9121..9500
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9531..9635
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9636..9704,9705..9740,1..756)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
       61 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      121 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      181 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      241 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      301 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      361 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      421 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
      481 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
      541 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
      601 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
      661 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
      721 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
      781 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
      841 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
      901 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
      961 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1021 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1081 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1141 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1201 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1261 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1321 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1381 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1441 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1501 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1561 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     1621 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     1681 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     1741 cattaatgca gcggatccag acatgataag atacattgat gagtttggac aaaccacaac
     1801 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     1861 aaccattata agctgcaata aacaagttag ctttttgcaa aagcctaggc ctccaaaaaa
     1921 gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat
     1981 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
     2041 gactatggtt gctgactaat tgagatgcgg atccgctggc acgacaggtt tcccgactgg
     2101 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     2161 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     2221 cacacaggaa acagctatga ccatgattac gccaagcttg ggctgcaggt cgagggatct
     2281 ccataagaga agagggacag ctatgactgg gagtagtcag gagaggagga aaaatctggc
     2341 tagtaaaaca tgtaaggaaa attttaggga tgttaaagaa aaaaataaca caaaacaaaa
     2401 tataaaaaaa atctaacctc aagtcaaggc ttttctatgg aataaggaat ggacagcagg
     2461 gggctgtttc atatactgat gacctcttta tagccaacct ttgttcatgg cagccagcat
     2521 atgggcatat gttgccaaac tctaaaccaa atactcattc tgatgtttta aatgatttgc
     2581 cctcccatat gtccttccga gtgagagaca caaaaaattc caacacacta ttgcaatgaa
     2641 aataaatttc ctttattagc cagaagtcag atgctcaagg ggcttcatga tgtccccata
     2701 atttttggca gagggaaaaa gatctcagtg gtatttgtga gccagggcat tggccacacc
     2761 agccaccacc ttctgatagg cagcctgcac ctgaggagtg cgctagcggt ggaagggctc
     2821 ggagttgtgg taatctatgt atcctgggcg gccgcctcta acggacttga gtgaggttgt
     2881 aaagggagtt ggctcggtac cgaattccgc tacagggcgc gtggggatac cccctagagc
     2941 cccagctggt tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc
     3001 tattgtcttc ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat
     3061 gacacctact cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag
     3121 tggcaccttc cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac
     3181 tagaaggcac agttacttgt acagctcgtc catcccacca ccaagcccgg cgaacttggc
     3241 aactgcgtgt tcgcgttgta ctacgaacat ttcattgtca ctttcctcta acctttccag
     3301 gcggtgatca acggcatgga taccgggcat cttgatgttc ccgacggtct tttttgacct
     3361 gtaagttgtt acgaaagagc aagacagatg gccaccacca tcaactttca gtgccatatg
     3421 agtgtatccc ctcagaccac catctgctgg atacatcatc tctgtattag gctcccaacc
     3481 cttggtcttc ttctgcatca cgggaccatt ggagggaaag tttacccctc tgacttggac
     3541 gtggtaaacg agacagccat cctcaaggct ggtgtcctgc atgacggtga cgactccacc
     3601 atcttcgtat ctcgtaactc tttcccaagt aaaaccctca ggaaaggact gtttaaagaa
     3661 atcaggaatg cctttcgggt acttgataaa agtacggctg ccatacatga acgacgtggc
     3721 aagaatgtca aaggcaaatg gcaggggtcc tccctcgatg actttgatcc tcatggtttg
     3781 agttcccatg tacggattgc cttctccttc acctgtgcat ttgaattggt ggccgttgac
     3841 cgaaccttcc atgaccacct tcatacgcat attttccttg atcagctctt cgcccttaga
     3901 caccatggtg gcggcaccgg tataacttcg tataatgtat gctatacgaa gttatacgcg
     3961 tgcgactttg cgtgttgtct tactattgct ggcaggaggt cagggtaccg aattccgcta
     4021 cagggcgcgt ggggataccc cctagagccc cagctggttc tttccgcctc agaagccata
     4081 gagcccaccg catccccagc atgcctgcta ttgtcttccc aatcctcccc cttgctgtcc
     4141 tgccccaccc caccccccag aatagaatga cacctactca gacaatgcga tgcaatttcc
     4201 tcattttatt aggaaaggac agtgggagtg gcaccttcca gggtcaagga aggcacgggg
     4261 gaggggcaaa caacagatgg ctggcaacta gaaggcacag tcactcttcc atcacgccga
     4321 tctgccaggc cagaacctgg gcgactagct cgcaggcttg gcggccatcg agatcgacgt
     4381 agtacggcgt tcggtgatgg caaacgatca atccccacag tcgctcgccg cgcaaaatcg
     4441 agatcgacat cgtgccgtgc atgcctatgt tgcgcatgaa ctccagatgg ttgggcgaga
     4501 cgctgcgcag gatggcgaag ctaagatcaa tcggccgccc ggtgaccgga ttgaggtctg
     4561 gggtgaccgg caccggccga taattgatat cgggaatgat ccgtaccggg ttgatggtat
     4621 agagccgacg ggcctgcgcc gggatgaatg aggcaggata gtgcaggcct agttttgact
     4681 cgacctcggc gcaccgatcc tctgcgatca cggacccgct gaagtcggag gcgaagcgat
     4741 agatcatcac ccgatcgaag ccggtaatct tccgcacctc ttgcgccgcg gcggcgcagg
     4801 cgctttccaa ggtttcggcg gcctgcaggc ggcggatggc gctgttggtg cggcggaaga
     4861 acgcctgcgg ctcggcgacg tcccgctggg gaggctcgag ctcgaggaag atgagctgat
     4921 catggcgatg ccaggagccg atgaagcctg cgtcctttcg catcgtgaag ccgacagtga
     4981 tcggtgctcc gacggccgcc ccgggctcgg ccaaggcgat cgtcagacgg ttgtgcgtct
     5041 ccgagtcgaa gacatcggcc gcagagcggc cgatcagggc gccgatcgcc agtccggtga
     5101 gttcgggaag gttgtcgctg ccggcaacga tcgtcatgtc ggcggcgagg gcgagcagca
     5161 gtccatgcgg ttggatggca ccggggatat ggatcggctc atcgtcgcag gtcaagaggt
     5221 caggctgcct ggcgacggat ccttccgcca tggtggcggc accggtataa cttcgtataa
     5281 tgtatgctat acgaagttat cgaagttcct atactttcta gagaatagga acttcacgcg
     5341 tgccgacctt gatgtttcca gtgcgattga ggaccttcag tgcggtaccg aattccgcta
     5401 cagggcgcgt ggggataccc cctagagccc cagctggttc tttccgcctc agaagccata
     5461 gagcccaccg catccccagc atgcctgcta ttgtcttccc aatcctcccc cttgctgtcc
     5521 tgccccaccc caccccccag aatagaatga cacctactca gacaatgcga tgcaatttcc
     5581 tcattttatt aggaaaggac agtgggagtg gcaccttcca gggtcaagga aggcacgggg
     5641 gaggggcaaa caacagatgg ctggcaacta gaaggcacag ttacttgtac agctcgtcca
     5701 tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct
     5761 cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg ggcagcagca
     5821 cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt
     5881 cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca
     5941 tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt
     6001 cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact
     6061 tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga
     6121 cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc
     6181 ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca
     6241 gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc
     6301 cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg
     6361 gcaccacccc ggtgaacagc tcctcgccct tgctcaccat ggtggcggca ccggtataac
     6421 ttcgtataaa gtatcctata cgaagttata cgcgtgcgct tggattctgc gtttgtttcc
     6481 gtctacgaac tcccagcggt accgaattcc gctacagggc gcgtggggat accccctaga
     6541 gccccagctg gttctttccg cctcagaagc catagagccc accgcatccc cagcatgcct
     6601 gctattgtct tcccaatcct cccccttgct gtcctgcccc accccacccc ccagaataga
     6661 atgacaccta ctcagacaat gcgatgcaat ttcctcattt tattaggaaa ggacagtggg
     6721 agtggcacct tccagggtca aggaaggcac gggggagggg caaacaacag atggctggca
     6781 actagaaggc acagtcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     6841 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     6901 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     6961 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     7021 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac
     7081 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     7141 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     7201 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     7261 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     7321 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     7381 agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc
     7441 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     7501 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     7561 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca
     7621 gatcaaatcc gataacttcg tataaagtat cctatacgaa gttatcgaag ttcctatact
     7681 ttctagagaa taggaacttc acgcgtgcga gacgagacga gacagcctga gaatggatgc
     7741 gagtaatggg cgcgccttcg ctcgaattaa tcaattcttt gccaaaatga tgagacagca
     7801 caataaccag cacgttgccc aggagctgta ggaaaaagaa gaaggcatga acatggttag
     7861 cagaggctct agagccgccg gtcacacgcc agaagccgaa ccccgccctg ccccgtcccc
     7921 cccgaaggca gccgtccccc cgcggacagc cccgaggctg gagagggaga aggggacggc
     7981 ggcgcggcga cgcacgaagg ccctccccgc ccatttcctt cctgccggcg ccgcaccgct
     8041 tcgccccgcg cccgctagag ggggtgcggc ggcgcctccc agatttcggc tccgcacaga
     8101 tttgggacaa aggaagtccc tgcgccctct cgcacgatta ccataaaagg caatggctgc
     8161 ggctcgccgc gcctcgacag ccgccggcgc tccgggggcc gccgcgcccc ycccccgagc
     8221 ccyccccggc ccgrggcggc cccgccccgc ccggcacccc cacctgccgc caccccccgc
     8281 ccggcacggc gagccccgcg ccacgccccg tacggagccc cgcacccgaa gccgggccgt
     8341 gctcagcaac tcggggaggg gggtgcaggg ggggttgcag cccgaccgac gcgcccacac
     8401 cccctgctca cccccccacg cacacacccc gcacgcagcc tttgttcccc tcgcagcccc
     8461 ccccgcaccg cggggcaccg cccccggccg cgctcccctc gcgcacactg cggagcgcac
     8521 aaagccccgc gccgcgcccg cagcgctcac agccgccggg cagcgcggag ccgcacgcgg
     8581 cgctccccac gcacacacac acgcacgcac cccccgagcc gctccccccg cacaaagggc
     8641 cctcccggag cccctcaagg ctttcacgca gccacagaaa agaaacaagc cgtcattaaa
     8701 ccaagcgcta attacagccc ggaggagaag ggccgtcccg cccgctcacc tgtgggagta
     8761 acgcggtcag tcagagccgg ggcgggcggc gcgaggcggc ggcggagcgg ggcacggggc
     8821 gaaggcagcg cgcagcgact cccgcccgcc gcgcgcttcg ctttttatag ggccgccgcc
     8881 gccgccgcct cgccataaaa ggaaactttc ggagcgcgcc gctctgattg gctgccgccg
     8941 cmccycyccs ccycsccccs ccccsccccc csccccsccc cccccccccy ggcgcgcgcc
     9001 cccccccccc cccgccccca tcgctgcaca aaataattaa aaaataaata aatacaaaat
     9061 tgggggtggg gagggggggg agatggggag agtgaagcag aacgtggggc tcacctcgac
     9121 catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt
     9181 catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt
     9241 acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac
     9301 ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga
     9361 cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt
     9421 aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata
     9481 actagtcaat aatcaatgtc gacccaggtg gcacttttcg gggaaatgtg cgcggaaccc
     9541 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     9601 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     9661 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     9721 tgaaagtaaa agatgctgaa
//
