LOCUS       sequence-46496-e       10634 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10634)
  AUTHORS   Longmire TA, Ikonomou L, Hawkins F, Christodoulou C, Cao Y, Jean JC,
            Kwok LW, Mou H, Rajagopal J, Shen SS, Dowton AA, Serra M, Weiss DJ, 
            Green MD, Snoeck HW, Ramirez MI, Kotton DN
  TITLE     Efficient derivation of purified lung and thyroid progenitors from 
            embryonic stem cells.
  JOURNAL   Cell Stem Cell. 2012 Apr 6;10(4):398-411.
  PUBMED    22482505
REFERENCE   2  (bases 1 to 10634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell."; date: "2012-04-6"; volume: "10(4)"; pages: "398-411"
COMMENT     Sequence Label: pHAGE hSPC-eGFP-W
FEATURES             Location/Qualifiers
     source          1..10634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2396..2418
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2410..2427
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2411..2427
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      6291..6300
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6297..6299,6300..6302,6303..7016)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6297..6299,6300..6302,6303..7013)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6342..6363)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6603..6622)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6950..6971
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7024..7612
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7077..7097)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7495..7506)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7687..7920
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8039..8057)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8125..8229
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8230..8298,8299..9090)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8448..8467)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9261..9849
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9750..9769
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10003..10020
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10260..10281
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10296..10301,10302..10319,10320..10326)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10334..10350
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10339..10361
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10358..10374
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10358..10374
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10424..10443
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtatg
     2221 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca
     2281 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     2341 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     2401 tcacgacgtt gtaaaacgac ggccagtgcc aagcttgaag actgctgctc tctaccacgt
     2461 tagctcccct gtggcggagg atgactgtcc taagagctca taggcacggg gcaaggggag
     2521 cctggctgtc agctgcctgg gcttctagtc ttgtgctttt tgcacaccag tccagggaac
     2581 gaagaccact ggctttaaga tgcttcccca gctgtcccca gactctgcca gcaggggatt
     2641 ctctggtctg agcttaagtt gtgttctccc agccagggat gcccctgccc tttgatgtct
     2701 ccttgctgcc acacatttag ccgccctccc catgccagct tggggggagg gaagcagtgg
     2761 gggtagggag gtggctgggg cagctgggca actgtcccca cccgtccctg gcacggctct
     2821 gcccagtaca caaagagcaa agtgaatctt gtccccaccc ctgcagctga ggggctggag
     2881 gaggaaacgg ggaggcccac acagaagggg tggccaccgt ggggctgtcc atcactcagg
     2941 gctctcagag ggagtcaacc cagaaacaga caaagagggt gagtctgggc tgtgttctta
     3001 gctagtgaga ggtcccctag aggatgaagt agatgatgct aatgaggatg actggatgtc
     3061 acacccatga tgctattagg tcctcataat agcatagtga ggtggacagc tagtacctga
     3121 cccatctcac agatgaacat actaatgcct aacaaagcag aacgactcac gctgggtccc
     3181 agagctggcc agtggaagca ctgagacctc cacatactga aggcatggac tattgaccgc
     3241 tgttggtatt ggtctcatca ttgactatca ttaagtgttg gctgtttgca tgcttcctgc
     3301 ccagtggcag gttcaaagaa gcccgcagga agcgtgctcc tttctttccc agggcccgca
     3361 attgggctgg aagatagagc aacaaaaagc gcccatgtaa ctcatgggaa cattcatgtg
     3421 tgctgaatgg caggtgaagg tgccacagag aggctgagga tttcagaggg caccatgaac
     3481 tggagtgagg tcgcagagca ggtgccattg gctcttggcc tgtttgggtg ggtggcattc
     3541 agagaggtgg aaggtcagat gcactgttca cgcctgtaat ctcagcactt tggaaggcca
     3601 aggtaggagg atcacacgag gccaggagat caacgctgca gtgagctatg aagctgtgat
     3661 tgcaccactg cactgcagct tgggtaacag agtgagaccc tgtctctaaa taattaaata
     3721 aataaaataa aaataaaacc ggagaagtgg agagggattg gaggtgggct ttcacagagg
     3781 gagaaacggc ttaagtacag gccaaaaagt gagaaggctg cagacagggc tggtaggggg
     3841 agggggaaat ttggcacacc cagctaaagg tccttctgtg tctccttctc caaggaaccc
     3901 aagaccttca cttggttggt gtgagcactc caggaggcag gcaccctccc tcagccctca
     3961 agcaagcaaa aatgggttta aaaaaagaag gagaagaagc agcagcagca gccgccacag
     4021 agcttgtgac agctacagcc taagggcaac aggcagggga gaccaaggac cagaaagagc
     4081 agaggctttt tcaaagaaag agatccctct cccagcaccc agcgatggcg gcaaacccca
     4141 cccacagtgc ctgctaagaa caagtcccac gtgagaacaa catggccccc cgagatgccc
     4201 acagggaccc cgagatgcct gcagtgcttg gctctcccgc tggccagctg cccacctggc
     4261 tcaggcccag tactcgtgag tcagccgatc aatcccaatg ttgccaggat gatgggggcg
     4321 ggagtaaggg ccctggggga gggcaggggt gggcactgca ggcgagctgt ctcccacatc
     4381 tggcacctgc acacagctga ggccgagctg agaggatgct tctgtgggct ccccctcctc
     4441 ccggcacccc tcccctcctt tcactgtcct aggacactct ctggctgctg gagtcttagg
     4501 caaatattta aaggggcagc aagggggtga ggagggtggt gggagcaaac acttccctcc
     4561 ctttcttcct ccctgcgctt ctcaggggct ctcagttcag atgccatgct gttatgcaac
     4621 cttggggctg aaggccctcc agatggagag ggggacaggg gaacctgcca gctcatgacc
     4681 gaagggcagg gcccaggtgg gagggggctg gggcagggga caggaactgg ggtggcatgt
     4741 taaaggacag gaggctggtt gggcatggtg gctcacacct gtaatcctag cactttagga
     4801 ggccgagatc acttgagccc aggagttcaa gaccagcctg ggcaacatgg tgaaaactca
     4861 tctctataaa acaagcaaaa attagctggg cacaatggca tgcactggta gtcccagcta
     4921 cttgggatgc tgaggtgtga ggatcaccgg agtccaggag gtcaacgctg cagtgagcag
     4981 tgatctcgct actgcatgcc agcctgggtg ataaagtgag accctgtctc acaacaaaac
     5041 aaaacaaaac aaaacaaaag gataggaggt taagggagcg agcccaggcc tggactctgc
     5101 cacagtcacc tgagtttaga ggtgaaggga ctttagagac cacctggccc aaggagtgaa
     5161 agggaacctg agagaaaggg tgagccagcc caaagtcatt ggcagatttg acctcgtaaa
     5221 tacatagaga tggctttggg aaggcactag gaaagacaga gaaaagagaa ggagacagtc
     5281 ctcaaagctg atcgtatttg ggtgagtatt attctcaggg caaatttagg atcagaggat
     5341 gcagaaaggg gagtctagag gggtagagtg tagaccacag ggtgagtgag ctgattcgag
     5401 gatggggaga ctgggagccc accagtgacc agagccagcc ctgttcaggg ctgtccgggc
     5461 agaagaaagc agtgtcagac ctggaatctg ccatcagcac agcctgcaat tgacagacaa
     5521 gcccagagca aagaaggaag cactgcacat gagtaagagc ttgccaccag tggggacaga
     5581 gtttccagaa ttaggaaaat aatcactggg ggcaagtttg aggttggtac cagatatgtg
     5641 ggaggaggca aggtaaggga aagagtactt gaagttggaa ctggtccttg cagggaaatg
     5701 cacatttatg aaaccccgaa aactgatgtc aaagcacctc ctgccttggg cagagtcctc
     5761 tcagagtcta caggtgctgc ctccagaacc ctcttcctgg agcgcatccc tatgtatcta
     5821 gaaattctgc tgggaaatat gatggtcaga cccttggcca cctgaaaggt tcagggtggt
     5881 agaagaaaaa ggaaagccac agggcagcag gggcaggtgc cagcaaggaa ggcaggcacg
     5941 ccaggaagac acccatggtg agaagtgcag atggcccgag ggcaagtttg ctcaactcac
     6001 ccaggtttgc tcttgctggg gccaagagga ctcatgtgcc agggccaagg gcccttgggg
     6061 gctctcacag ggggcttatc tgggcttcgg ttctggaggg ccaggaacaa acaggcttca
     6121 aagccaaggg cttggctggc acacaggggg cttggtcctt cacctctgtc ccctctccct
     6181 acggacacat ataagaccct ggtcacacct gggagaggag gagaggagag catagcacct
     6241 gcaggtcgac tctagaggat cgatccccgg gtaccgagct cgaattagcg gccgccatgg
     6301 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     6361 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     6421 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     6481 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     6541 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     6601 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     6661 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     6721 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     6781 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     6841 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     6901 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     6961 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaggat
     7021 cctaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     7081 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     7141 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     7201 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     7261 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     7321 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     7381 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     7441 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     7501 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     7561 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgagatc
     7621 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     7681 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     7741 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     7801 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     7861 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     7921 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     7981 agagtgagag gcccgggtta attaaggaaa gggctagatc attcttgaag acgaaagggc
     8041 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     8101 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     8161 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     8221 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     8281 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     8341 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     8401 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     8461 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     8521 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     8581 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     8641 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     8701 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     8761 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     8821 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     8881 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     8941 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     9001 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     9061 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     9121 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     9181 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     9241 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     9301 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     9361 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     9421 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     9481 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     9541 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     9601 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     9661 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     9721 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     9781 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     9841 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     9901 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     9961 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
    10021 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
    10081 tgcagcaagc tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
    10141 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
    10201 agctccccgt ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
    10261 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
    10321 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt
    10381 acgaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    10441 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc
    10501 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa
    10561 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
    10621 acaaacttag tagt
//
