LOCUS       sequence-464962-        7872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Red light-inducible Cre-C; Cre-106C-PhyA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7872)
  AUTHORS   Tous C, Kinstlinger IS, Rice MEL, Deng J, Wong WW
  TITLE     Multiplexing light-inducible recombinases to control cell fate, 
            Boolean logic, and cell patterning in mammalian cells.
  JOURNAL   Sci Adv. 2025 May 9;11(19):eadt1971. doi: 10.1126/sciadv.adt1971. 
            Epub 2025 May 9.
  PUBMED    40344052
REFERENCE   2  (bases 1 to 7872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-05-9"; volume: "11(19):eadt1971. doi"; pages: " 
            10.1126/sciadv.adt1971. Epub 2025 May 9"
COMMENT     Sequence Label: CT361
FEATURES             Location/Qualifiers
     source          1..7872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1460..1480
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1490..1714
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1954..1973)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2019..2074
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2073..2092)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2435..2451)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2435..2451)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2448..2470)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2459..2475
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2483..2489,2490..2507,2508..2513))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2528..2549
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      2634..2768
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2788..2922
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2825..2844)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2879..2898
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2990..3007)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3161..3749)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3241..3260)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3920..4711,4712..4780))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4543..4562
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4781..4885)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4916..5295
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5297..5573
                     /label=chicken beta-actin promoter
     intron          5574..6590
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     6514..6536
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     6598..6617
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      6719..6728
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cccttttccc ttacgttatg cttgtgaatt tttagcccaa gtgtttgcca tccacgtcaa
       61 caaggaggtc gagctggaca accagatggt ggagaagaac attttacgta cccagacact
      121 cctctgcgac atgctcatga gggacgctcc cctcggcatc gtgagccagt cccccaatat
      181 tatggattta gtcaagtgcg acggcgccgc cttattatac aaggacaaga tctggaagct
      241 gggcaccacc cctagcgagt tccatttaca agaaatcgct tcttggctgt gtgagtacca
      301 catggattcc accggtttaa gcaccgattc tttacacgac gctggctttc ctagggcttt
      361 atctttaggc gacagcgtct gcggaatggc tgccgttcgt atcagcagca aagatatgat
      421 tttctggttt cgttcccata ccgccggcga agtgagatgg ggcggcgcca aacacgaccc
      481 cgatgatagg gacgatgcca gaaggatgca ccccagatcc tccttcaagg cttttctgga
      541 ggtggtgaag accagaagct taccttggaa ggactacgaa atggatgcca tccactcttt
      601 acagctcatc ttacgtaacg cctttaagga cagcgagacc accgacgtga acacaaaggt
      661 gatctatagc aagctcaacg atctcaagat tgatggaatc caatccggag ggtctggctc
      721 cggatcaagt ggtggcagcg gtacccgccc ttctgactcc aatgctgtgt ccctggtgat
      781 gaggagaatc agaaaggaga atgtggatgc tggggagaga gccaagcagg ccctggcctt
      841 tgaacgcact gactttgacc aagtcagatc cctgatggag aactctgaca gatgccagga
      901 catcaggaac ctggccttcc tgggcattgc ctacaacacc ctgctgcgca ttgccgaaat
      961 tgccagaatc agagtgaagg acatctcccg caccgatggt gggagaatgc tgatccacat
     1021 tggcaggacc aagaccctgg tgtccacagc tggtgtggag aaggccctgt ccctgggggt
     1081 taccaagctg gtggagagat ggatctctgt gtctggtgtg gctgatgacc ccaacaacta
     1141 cctgttctgc cgggtcagaa agaatggtgt ggctgcccct tctgccacct cccaactgtc
     1201 cacccgggcc ctggaaggga tctttgaggc cacccaccgc ctgatctatg gtgccaagga
     1261 tgactctggg cagagatacc tggcctggtc tggccactct gccagagtgg gtgctgccag
     1321 ggacatggcc agggctggtg tgtccatccc tgaaatcatg caggctggtg gctggaccaa
     1381 tgtgaacatt gtgatgaact acatcagaaa cctggactct gagactgggg ccatggtgag
     1441 gctgctcgag gatggggacc ccaagaaaaa gcggaaggtg tgagaattcc tgtgccttct
     1501 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     1561 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     1621 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     1681 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     1741 ggctctaggg ggtatcccca cgcgccctgt agcgtgtacc gctgggagtt cgtagacgga
     1801 aacaaacgca gaatccaagc gcggccgccc aggatacata gattaccaca actccgagcc
     1861 cttccaccgc tagcgcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt
     1921 ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg
     1981 ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat
     2041 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
     2101 tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct
     2161 ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc
     2221 tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg
     2281 ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag
     2341 atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc
     2401 cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     2461 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     2521 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     2581 cgggaaacct gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc
     2641 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     2701 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     2761 tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa
     2821 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     2881 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg
     2941 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     3001 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     3061 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     3121 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     3181 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     3241 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     3301 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     3361 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     3421 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     3481 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     3541 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     3601 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     3661 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     3721 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     3781 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     3841 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     3901 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     3961 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     4021 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     4081 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     4141 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     4201 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     4261 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     4321 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     4381 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     4441 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     4501 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     4561 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     4621 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     4681 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     4741 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     4801 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     4861 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtcgacat
     4921 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     4981 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     5041 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     5101 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     5161 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     5221 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     5281 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
     5341 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg
     5401 gcgggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg
     5461 cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta
     5521 tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg
     5581 ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc
     5641 tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct
     5701 gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg
     5761 aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg
     5821 tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc
     5881 gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg
     5941 ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg
     6001 gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc
     6061 cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg
     6121 ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc
     6181 ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc
     6241 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
     6301 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta
     6361 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
     6421 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg
     6481 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     6541 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     6601 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attgattaat tcgagcgaag
     6661 gcgcgcccat tactcgcatc cattctcagg ctgtctcgtc tcgtctcgca cgcgtgccgc
     6721 caccatggag aagaagatga gcggatctcg tcccacacag tccagcgagg gatctcgtag
     6781 atctcgtcac tccgctcgta tcatcgctca gaccaccgtg gacgccaaac tgcacgccga
     6841 tttcgaggag agcggctcct cctttgatta ctccaccagc gtgagggtga ctggtcccgt
     6901 ggtcgagaac cagcctccta ggagcgacaa ggtcaccaca acctacctcc atcatatcca
     6961 gaagggcaag ctgatccagc cctttggatg tttactggct ttagacgaaa agaccttcaa
     7021 ggtcatcgcc tactccgaga acgcctccga actgctcaca atggcttccc acgccgtgcc
     7081 tagcgtggga gagcaccccg ttctgggcat cggcaccgat atcagatctt tatttaccgc
     7141 tcccagcgct tccgctttac agaaggctct cggcttcggc gacgtgtctt tactgaaccc
     7201 tatcctcgtc cattgtcgta catccgccaa gcccttctac gccattatcc atagggtgac
     7261 cggatccatc atcatcgact tcgaacccgt taagccctat gaagtgccca tgacagccgc
     7321 cggagcttta cagagctata agctggccgc caaggccatc accagactcc agtctttacc
     7381 cagcggaagc atggagaggc tgtgcgacac catggtgcaa gaagtcttcg agctgactgg
     7441 ttacgatcgt gtcatggcct acaagttcca cgaggacgac cacggcgagg tggtgagcga
     7501 agtgaccaag cccggtttag aaccctattt aggtttacac tatcccgcca cagacatccc
     7561 ccaagctgcc agatttttat ttatgaagaa caaggtgaga atgatcgtgg actgcaacgc
     7621 caagcacgct agggttttac aagatgagaa gctgagcttc gatttaaccc tctgcggcag
     7681 cactttaaga gctccccact cttgtcatct gcaatacatg gccaatatgg acagcatcgc
     7741 ttctttagtg atggccgtgg tggtgaatga ggaggatgga gagggcgatg ctcccgatgc
     7801 caccacacag cctcagaaga gaaagaggct gtggggttta gtggtctgcc acaacaccac
     7861 ccccagattt gt
//
