LOCUS       sequence-464963-        7239 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Red light-inducible Flp-C; Flp-397C-PhyA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Tous C, Kinstlinger IS, Rice MEL, Deng J, Wong WW
  TITLE     Multiplexing light-inducible recombinases to control cell fate, 
            Boolean logic, and cell patterning in mammalian cells.
  JOURNAL   Sci Adv. 2025 May 9;11(19):eadt1971. doi: 10.1126/sciadv.adt1971. 
            Epub 2025 May 9.
  PUBMED    40344052
REFERENCE   2  (bases 1 to 7239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-05-9"; volume: "11(19):eadt1971. doi"; pages: " 
            10.1126/sciadv.adt1971. Epub 2025 May 9"
COMMENT     Sequence Label: CT445
FEATURES             Location/Qualifiers
     source          1..7239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             810..830
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    840..1064
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1304..1323)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1369..1424
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1423..1442)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(1785..1801)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1785..1801)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1798..1820)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1809..1825
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1833..1839,1840..1857,1858..1863))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1878..1899
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      1984..2118
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2138..2272
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2175..2194)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2229..2248
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2340..2357)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2511..3099)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2591..2610)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3270..4061,4062..4130))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3893..3912
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4131..4235)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4266..4645
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4647..4923
                     /label=chicken beta-actin promoter
     intron          4924..5940
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5864..5886
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     5948..5967
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      6069..6078
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgcttgtga atttttagcc caagtgtttg ccatccacgt caacaaggag gtcgagctgg
       61 acaaccagat ggtggagaag aacattttac gtacccagac actcctctgc gacatgctca
      121 tgagggacgc tcccctcggc atcgtgagcc agtcccccaa tattatggat ttagtcaagt
      181 gcgacggcgc cgccttatta tacaaggaca agatctggaa gctgggcacc acccctagcg
      241 agttccattt acaagaaatc gcttcttggc tgtgtgagta ccacatggat tccaccggtt
      301 taagcaccga ttctttacac gacgctggct ttcctagggc tttatcttta ggcgacagcg
      361 tctgcggaat ggctgccgtt cgtatcagca gcaaagatat gattttctgg tttcgttccc
      421 ataccgccgg cgaagtgaga tggggcggcg ccaaacacga ccccgatgat agggacgatg
      481 ccagaaggat gcaccccaga tcctccttca aggcttttct ggaggtggtg aagaccagaa
      541 gcttaccttg gaaggactac gaaatggatg ccatccactc tttacagctc atcttacgta
      601 acgcctttaa ggacagcgag accaccgacg tgaacacaaa ggtgatctat agcaagctca
      661 acgatctcaa gattgatgga atccaatccg gagggtctgg ctccggatca agtggtggca
      721 gcggtaccag catcagatac cccgcctgga acggcatcat cagccaggag gtgctggact
      781 acctgagcag ctacatcaac aggcggatcc ccaagaaaaa gcggaaggtg tgagaattcc
      841 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
      901 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
      961 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     1021 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     1081 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcgtgtacc gctgggagtt
     1141 cgtagacgga aacaaacgca gaatccaagc gcggccgccc aggatacata gattaccaca
     1201 actccgagcc cttccaccgc tagcgcactc ctcaggtgca ggctgcctat cagaaggtgg
     1261 tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca
     1321 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat
     1381 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
     1441 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg
     1501 cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac
     1561 agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt
     1621 ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt
     1681 tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct
     1741 tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct
     1801 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     1861 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     1921 gctttccagt cgggaaacct gtcgtgccag cggatccgca tctcaattag tcagcaacca
     1981 tagtcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     2041 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     2101 gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa
     2161 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     2221 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat
     2281 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     2341 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     2401 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     2461 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2521 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2581 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2641 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     2701 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     2761 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     2821 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2881 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     2941 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     3001 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     3061 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     3121 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     3181 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     3241 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     3301 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3361 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     3421 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3481 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3541 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     3601 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3661 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     3721 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     3781 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     3841 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     3901 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     3961 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     4021 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     4081 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     4141 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     4201 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     4261 gggtcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     4321 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     4381 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     4441 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     4501 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     4561 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     4621 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
     4681 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
     4741 agcgatgggg gcgggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg
     4801 cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
     4861 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
     4921 gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc
     4981 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
     5041 cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt
     5101 gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg
     5161 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag
     5221 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc
     5281 gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt
     5341 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg
     5401 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg
     5461 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
     5521 gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc
     5581 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg
     5641 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc
     5701 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg
     5761 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt
     5821 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
     5881 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag
     5941 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attgattaat
     6001 tcgagcgaag gcgcgcccat tactcgcatc cattctcagg ctgtctcgtc tcgtctcgca
     6061 cgcgtgccgc caccatggag aagaagatga gcggatctcg tcccacacag tccagcgagg
     6121 gatctcgtag atctcgtcac tccgctcgta tcatcgctca gaccaccgtg gacgccaaac
     6181 tgcacgccga tttcgaggag agcggctcct cctttgatta ctccaccagc gtgagggtga
     6241 ctggtcccgt ggtcgagaac cagcctccta ggagcgacaa ggtcaccaca acctacctcc
     6301 atcatatcca gaagggcaag ctgatccagc cctttggatg tttactggct ttagacgaaa
     6361 agaccttcaa ggtcatcgcc tactccgaga acgcctccga actgctcaca atggcttccc
     6421 acgccgtgcc tagcgtggga gagcaccccg ttctgggcat cggcaccgat atcagatctt
     6481 tatttaccgc tcccagcgct tccgctttac agaaggctct cggcttcggc gacgtgtctt
     6541 tactgaaccc tatcctcgtc cattgtcgta catccgccaa gcccttctac gccattatcc
     6601 atagggtgac cggatccatc atcatcgact tcgaacccgt taagccctat gaagtgccca
     6661 tgacagccgc cggagcttta cagagctata agctggccgc caaggccatc accagactcc
     6721 agtctttacc cagcggaagc atggagaggc tgtgcgacac catggtgcaa gaagtcttcg
     6781 agctgactgg ttacgatcgt gtcatggcct acaagttcca cgaggacgac cacggcgagg
     6841 tggtgagcga agtgaccaag cccggtttag aaccctattt aggtttacac tatcccgcca
     6901 cagacatccc ccaagctgcc agatttttat ttatgaagaa caaggtgaga atgatcgtgg
     6961 actgcaacgc caagcacgct agggttttac aagatgagaa gctgagcttc gatttaaccc
     7021 tctgcggcag cactttaaga gctccccact cttgtcatct gcaatacatg gccaatatgg
     7081 acagcatcgc ttctttagtg atggccgtgg tggtgaatga ggaggatgga gagggcgatg
     7141 ctcccgatgc caccacacag cctcagaaga gaaagaggct gtggggttta gtggtctgcc
     7201 acaacaccac ccccagattt gtcccttttc ccttacgtt
//
