LOCUS       sequence-464965-        9467 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9467)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 9467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag HIPK1
FEATURES             Location/Qualifiers
     source          1..9467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    425..624
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             825..1241
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1171..1193
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1209..1225
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1259..1268
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1265..1306
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             1331..1354
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1361..1385
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2887..2911)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2970..3431
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3026..3043)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3253..3272
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3436..4230
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3490..3509)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4100..4119
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             4417..4886
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        4922..5026
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5027..5095,5096..5887)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5245..5264)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7653..8241
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8142..8161
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8395..8412
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8516..8943)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8626..8647)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8838..8857
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     enhancer        9088..9467
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgcg ccagtcctcc gattgactga
      241 gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct
      301 cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca
      361 tttgggggct cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg
      421 aggcaagctg gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt
      481 ttatgcgcct gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg
      541 gaactgacga gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg
      601 gccgtttttg tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat
      661 atgtggttct ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc
      721 tttcggtttg gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt
      781 gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa ttagggccag actgttacca
      841 ctcccttaag tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt
      901 cggtagatgt caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta
      961 acgtcggatg gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca
     1021 aggtcttttc acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg
     1081 aagccttggc ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc
     1141 ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc
     1201 gatcctccct ttatccagcc ctcactcctt ctctaggcgc cggccggatc cagaattcgc
     1261 caccatgggg tcttcaaaat ctaaaccaaa ggaccccagc cagcgccggc gcaggatccg
     1321 aggttacctt gactacaaag acgatgacga caagcaattg acaagtttgt acaaaaaagt
     1381 tggcatggtt ttgatgtttc agattcgtta tatttcacaa acacaaggct tgccagctga
     1441 atatcttctc agtgccggaa caaaaacaac caggtttttc aacagagatc ctaatttggg
     1501 gtacccactg tggaggctta agacacctga agaacatgaa ctggagactg gaataaaatc
     1561 aaaagaagct cggaagtaca tttttaattg cttagatgac atggctcagg tgaatatgtc
     1621 tacggacctg gagggaacag acatgttggc agagaaggca gaccgaagag aatacattga
     1681 tctgttaaag aaaatgctca caattgatgc agataagaga attacccctc taaaaactct
     1741 taaccatcag tttgtgacaa tgactcacct tttggatttt ccacatagca atcatgttaa
     1801 gtcttgtttt cagaacatgg agatctgcaa gcggagggtt cacatgtatg atacagtgag
     1861 tcagatcaag agtcccttca ctacacatgt tgccccaaat acaagcacaa atctaaccat
     1921 gagcttcagc aatcagctca atacagtgca caatcaggcc agtgttctag cttccagttc
     1981 tactgcagca gctgctactc tttctctggc taattcagat gtctcactac taaactacca
     2041 gtcagctttg tacccatcat ctgctgcacc agttcctgga gttgcccagc agggtgtttc
     2101 cttgcagcct ggaaccaccc agatttgcac tcagacagat ccattccaac agacatttat
     2161 agtatgtcca cctgcgtttc aaactggact acaagcaaca acaaagcatt ctggattccc
     2221 tgtgaggatg gataatgctg taccaattgt accccaggca ccagctgctc agccactaca
     2281 gattcagtca ggagttctca cgcagggaag ctgtacacca ctaatggtag caactctcca
     2341 ccctcaagta gccaccatca caccgcagta tgcggtgccc tttactctga gctgcgcagc
     2401 cggccggccg gcgctggttg aacagactgc cgctgtactg caggcgtggc ctggagggac
     2461 tcagcaaatt ctcctgcctt caacttggca acagttgcct ggggtagctc tacacaactc
     2521 tgtccagccc acagcaatga ttccagaggc catggggagt ggacagcagc tagctgactg
     2581 gaggaatgcc cactctcatg gcaaccagta cagcactatc atgcagcagc catccttgct
     2641 gactaaccat gtgacattgg ccactgctca gcctctgaat gttggtgttg cccatgttgt
     2701 cagacaacaa caatccagtt ccctcccttc gaagaagaat aagcagtcag ctccagtctc
     2761 ttccaagtcc tctctagatg ttctgccttc ccaagtctat tctctggttg ggagcagtcc
     2821 cctccgcacc acatcttctt ataattcctt ggtccctgtc caagatcagc cagtattcct
     2881 acttataccc aactttcttg tacaaagtgg tttgatctag tcgaccaatt ccggttattt
     2941 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     3001 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     3061 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     3121 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     3181 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     3241 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     3301 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     3361 tcgaggttaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     3421 cacgatgata ataccatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     3481 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     3541 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     3601 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     3661 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     3721 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     3781 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     3841 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     3901 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     3961 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     4021 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     4081 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     4141 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     4201 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgca tcgataaaat
     4261 aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg
     4321 gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa ctgagaatag
     4381 agaagttcag atcaaggtca ggaacagatg gaacagctga atatgggcca aacaggatat
     4441 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggaaca gctgaatatg
     4501 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
     4561 gtccccagat gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt
     4621 gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc
     4681 gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc
     4741 ggggcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc
     4801 ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt
     4861 gactacccgt cagcgggggt ctttcacagg ccagtggggc cgcacttttc ggggaaatgt
     4921 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     4981 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5041 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5101 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5161 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5221 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     5281 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5341 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5401 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5461 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5521 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     5581 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     5641 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     5701 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     5761 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     5821 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     5881 ttggtaactg tcagaccaag tttactcata tatactttag attgatttgc ggccgcgaaa
     5941 aggacaagca gcgaaaattc acgccccctt gggaggtggc ggcatatgca aaggatagca
     6001 ctcccactct actactgggt atcatatgct gactgtatat gcatgaggat agcatatgct
     6061 acccggatac agattaggat agcatatact acccagatat agattaggat agcatatgct
     6121 acccagatat agattaggat agcctatgct acccagatat aaattaggat agcatatact
     6181 acccagatat agattaggat agcatatgct acccagatat agattaggat agcctatgct
     6241 acccagatat agattaggat agcatatgct atccagatat ttgggtagta tatgctaccc
     6301 atggcaacat tagcccaccg tgctctcagc gacctcgtga atatgaggac caacaaccct
     6361 gtgcttggcg ctcaggcgca agtgtgtgta atttgtcctc cagatcgcag caatcgcgcc
     6421 cctatcttgg cccgcccacc tacttatgca ggtattcccc ggggtgccat tagtggtttt
     6481 gtgggcaagt ggtttgaccg cagtggttag cggggttaca atcagccaag ttattacacc
     6541 cttattttac agtccaaaac cgcagggcgg cgtgtggggg ctgacgcgtg cccccactcc
     6601 acaatttcaa aaaaaagagt ggccacttgt ctttgtttat gggccccatt ggcgtggagc
     6661 cccgtttaat tttcgggggt gttagagaca accagtggag tccgctgctg tcggcgtcca
     6721 ctctctttcc ccttgttaca aatagagtgt aacaacatgg ttcacctgtc ttggtccctg
     6781 cctgggacac atcttaataa ccccagtatc atattgcact aggattatgt gttgcccata
     6841 gccataaatt cgtgtgagat ggacatccag tctttacggc ttgtccccac cccatggatt
     6901 tctattgtta aagatattca gaatgtttca ttcctacact agtatttatt gcccaagggg
     6961 tttgtgaggg ttatattggt gtcatagcac aatgccacca ctgaaccccc cgtccaaatt
     7021 ttattctggg ggcgtcacct gaaaccttgt tttcgagcac ctcacataca ccttactgtt
     7081 cacaactcag cagttattct attagctaaa cgaaggagaa tgaagaagca ggcgaagatt
     7141 caggagagtt cactgcccgc tccttgatct tcagccactg cccttgtgac taaaatggtt
     7201 cactaccctc gtggaatcct gaccccatgt aaataaaacc gtgacagctc atggggtggg
     7261 agatatcgct gttccttagg acccttttac taaccctaat tcgatagcat atgcttcccg
     7321 ttgggtaaca tatgctattg aattagggtt agtctggata gtatatacta ctacccggga
     7381 agcatatgct acccgtttag ggttaacaag ggggccttat aaacactatt gctaatgccc
     7441 tcttgagggt ccgcttatcg gtagctacac aggcccctct gattgacgtt ggtgtagcct
     7501 cccgtagtct tcctgggccc ctgggaggta catgtcggcc gcaaaaggat ctaggtgaag
     7561 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     7621 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     7681 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     7741 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     7801 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7861 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7921 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7981 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     8041 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     8101 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     8161 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     8221 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     8281 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     8341 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     8401 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     8461 ccgattcatt aatgcaacta tggttggcgc gttgtaaacg ttaatatttt gttaaaattc
     8521 gcgttaaatt tttgttaaat cagctcattt tttaaccaat agaccgaaat cggcaaaatc
     8581 ccttataaat caaaagaata gcccgagata gagttgagtg ttgttccagt ttggaacaag
     8641 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     8701 gatggcccac tacgtgaacc atcacccaaa tcaagttttt tggggtcgag gtgccgtaaa
     8761 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     8821 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctaaggc gctggcaagt
     8881 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     8941 gcgccgatct tcaatattgg ccattagcca tattattcat tggttatata gcataaatca
     9001 atattggcta ttggccattg catacgttgt atctatatca taatatgtac atttatattg
     9061 gctcatgtcc aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat
     9121 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     9181 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     9241 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     9301 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg
     9361 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact
     9421 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatg
//
