LOCUS       sequence-464969-         315 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector that can express doxycycline-inducible hAqp1 with 
            fkbp12 degron in Tet ON mammalian cells. CMVtight promoter. EGFP 
            marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Yun J, Huang Y, Miller ADC, Chang BL, Baldini L, Dhanabalan KM, Li 
            E, Li H, Mukherjee A
  TITLE     Destabilized reporters for background-subtracted, chemically-gated, 
            and multiplexed deep-tissue imaging.
  JOURNAL   Chem Sci. 2024 Jun 4;15(28):11108-11121. doi: 10.1039/d4sc00377b. 
            eCollection 2024 Jul 17.
  PUBMED    39027298
REFERENCE   2  (bases 1 to 315)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/d4sc00377b"; journalName: "Chem Sci"; date: "2024-06-4- 4";
            volume: "15"; issue: "28"; pages: "11108-11121"
COMMENT     Sequence Label: pL(CMVtight-phi-AQP1-FKBP12DD-IRES-EGFP-WPRE)
FEATURES             Location/Qualifiers
     source          1..315
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..315
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    9..27
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    45..63
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    80..98
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    116..134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    152..170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    187..205
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    223..241
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     288..312
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
       61 agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct
      121 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
      181 agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
      241 aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
      301 aaccgtcaga tcgcc
//
