LOCUS       sequence-464971-         587 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector that can express doxycycline-inducible hAqp1 with 
            fkbp12 degron in Tet ON mammalian cells. CMVtight promoter. EGFP 
            marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Yun J, Huang Y, Miller ADC, Chang BL, Baldini L, Dhanabalan KM, Li 
            E, Li H, Mukherjee A
  TITLE     Destabilized reporters for background-subtracted, chemically-gated, 
            and multiplexed deep-tissue imaging.
  JOURNAL   Chem Sci. 2024 Jun 4;15(28):11108-11121. doi: 10.1039/d4sc00377b. 
            eCollection 2024 Jul 17.
  PUBMED    39027298
REFERENCE   2  (bases 1 to 587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/d4sc00377b"; journalName: "Chem Sci"; date: "2024-06-4- 4";
            volume: "15"; issue: "28"; pages: "11108-11121"
COMMENT     Sequence Label: pL(CMVtight-phi-AQP1-FKBP12DD-IRES-EGFP-WPRE)
FEATURES             Location/Qualifiers
     source          1..587
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(1..575,576..578,579..587)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(168..185)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     395..414
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
       61 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
      121 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
      181 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
      241 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
      301 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
      361 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
      421 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
      481 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
      541 ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaacc
//
