LOCUS       sequence-46498-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Hong EL, Shinohara A, Bishop DK
  TITLE     Saccharomyces cerevisiae Dmc1 protein promotes renaturation of 
            single-strand DNA (ssDNA) and assimilation of ssDNA into homologous 
            super-coiled duplex DNA.
  JOURNAL   J Biol Chem. 2001 Nov 9;276(45):41906-12. Epub 2001 Sep 10.
  PUBMED    11551925
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2001-11-9"; volume: "276(45)"; pages: "41906-12. Epub
            2001 Sep 10"
COMMENT     Sequence Label: pNRB150
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     59..78
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        59..77
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    78..102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             117..139
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             150..167
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cggggagccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaatta
       61 atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg
      121 tttaacttta agaaggagat atacatatgc accatcatca ccatcattct gttacaggaa
      181 ctgagatcga tagtgataca gcaaaaaata ttcttagtgt agatgaatta cagaactatg
      241 gtattaatgc ctcagatctt caaaaattga agtctggtgg gatatacaca gtcaataccg
      301 ttttgtcaac aacaagaaga catctatgta aaattaaagg gttaagtgag gtgaaagtgg
      361 aaaaaattaa agaagctgct ggaaaaatca tacaggtggg atttatcccc gctactgtac
      421 aattagatat aagacagcgt gtgtactcct tatcaactgg atctaagcaa ctagattcaa
      481 tcctaggtgg tggaataatg acaatgagta tcactgaagt atttggtgaa tttaggtgtg
      541 gtaagacaca gatgtctcat actttgtgtg ttaccacgca gcttccgagg gaaatgggtg
      601 gtggtgaagg gaaagtagca tatattgata cagaaggcac tttcaggccc gagaggatta
      661 agcaaattgc agaaggttat gaattggatc ccgagtcatg tttggcaaac gtttcatatg
      721 ctagagcctt gaatagtgaa catcaaatgg aacttgttga acaattgggt gaagaactta
      781 gttctggaga ttatcgcctt atcgtggtag attctataat ggcaaacttc agagtagact
      841 actgcggtag aggtgaacta agcgaaagac agcaaaagct aaatcaacat cttttcaaat
      901 tgaatagatt ggcagagga
//
