LOCUS       sequence-464994-        7843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Red light-inducible Cre-N; Cre-N229-PhyA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7843)
  AUTHORS   Tous C, Kinstlinger IS, Rice MEL, Deng J, Wong WW
  TITLE     Multiplexing light-inducible recombinases to control cell fate, 
            Boolean logic, and cell patterning in mammalian cells.
  JOURNAL   Sci Adv. 2025 May 9;11(19):eadt1971. doi: 10.1126/sciadv.adt1971. 
            Epub 2025 May 9.
  PUBMED    40344052
REFERENCE   2  (bases 1 to 7843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-05-9"; volume: "11(19):eadt1971. doi"; pages: " 
            10.1126/sciadv.adt1971. Epub 2025 May 9"
COMMENT     Sequence Label: CT616
FEATURES             Location/Qualifiers
     source          1..7843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        32..136
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(137..205,206..997)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(355..374)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1168..1756
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1657..1676
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1910..1927
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    1995..2129
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2019..2038)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2073..2092
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      2149..2283
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    2368..2389
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2404..2409,2410..2427,2428..2434)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2442..2458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2447..2469
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2466..2482
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2466..2482
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2825..2844
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    2843..2898
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     2944..2963
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3203..3427
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3437..3457)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6142..6161)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          6169..7185
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(6223..6245)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(7186..7463)
                     /label=chicken beta-actin promoter
     enhancer        join(7465..7843,1)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cgacccaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
       61 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      121 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      181 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      241 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
      301 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      361 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      421 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      481 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
      541 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
      601 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
      661 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
      721 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
      781 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
      841 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
      901 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
      961 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     1021 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1081 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1141 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1201 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1261 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     1321 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1381 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1441 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1501 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1561 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1621 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1681 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1741 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1801 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     1861 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     1921 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     1981 tcattaatgc agcggatcca gacatgataa gatacattga tgagtttgga caaaccacaa
     2041 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     2101 taaccattat aagctgcaat aaacaagtta gctttttgca aaagcctagg cctccaaaaa
     2161 agcctcctca ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa
     2221 taaaaaaaat tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg
     2281 ggactatggt tgctgactaa ttgagatgcg gatccgctgg cacgacaggt ttcccgactg
     2341 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     2401 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     2461 tcacacagga aacagctatg accatgatta cgccaagctt gggctgcagg tcgagggatc
     2521 tccataagag aagagggaca gctatgactg ggagtagtca ggagaggagg aaaaatctgg
     2581 ctagtaaaac atgtaaggaa aattttaggg atgttaaaga aaaaaataac acaaaacaaa
     2641 atataaaaaa aatctaacct caagtcaagg cttttctatg gaataaggaa tggacagcag
     2701 ggggctgttt catatactga tgacctcttt atagccaacc tttgttcatg gcagccagca
     2761 tatgggcata tgttgccaaa ctctaaacca aatactcatt ctgatgtttt aaatgatttg
     2821 ccctcccata tgtccttccg agtgagagac acaaaaaatt ccaacacact attgcaatga
     2881 aaataaattt cctttattag ccagaagtca gatgctcaag gggcttcatg atgtccccat
     2941 aatttttggc agagggaaaa agatctcagt ggtatttgtg agccagggca ttggccacac
     3001 cagccaccac cttctgatag gcagcctgca cctgaggagt gcgctagcgg tggaagggct
     3061 cggagttgtg gtaatctatg tatcctgggc ggccgcgctt ggattctgcg tttgtttccg
     3121 tctacgaact cccagcggta cacgctacag ggcgcgtggg gataccccct agagccccag
     3181 ctggttcttt ccgcctcaga agccatagag cccaccgcat ccccagcatg cctgctattg
     3241 tcttcccaat cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac
     3301 ctactcagac aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca
     3361 ccttccaggg tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa
     3421 ggcacaggaa ttctcacacc ttccgctttt tcttgggttg gattccatca atcttgagat
     3481 cgttgagctt gctatagatc acctttgtgt tcacgtcggt ggtctcgctg tccttaaagg
     3541 cgttacgtaa gatgagctgt aaagagtgga tggcatccat ttcgtagtcc ttccaaggta
     3601 agcttctggt cttcaccacc tccagaaaag ccttgaagga ggatctgggg tgcatccttc
     3661 tggcatcgtc cctatcatcg gggtcgtgtt tggcgccgcc ccatctcact tcgccggcgg
     3721 tatgggaacg aaaccagaaa atcatatctt tgctgctgat acgaacggca gccattccgc
     3781 agacgctgtc gcctaaagat aaagccctag gaaagccagc gtcgtgtaaa gaatcggtgc
     3841 ttaaaccggt ggaatccatg tggtactcac acagccaaga agcgatttct tgtaaatgga
     3901 actcgctagg ggtggtgccc agcttccaga tcttgtcctt gtataataag gcggcgccgt
     3961 cgcacttgac taaatccata atattggggg actggctcac gatgccgagg ggagcgtccc
     4021 tcatgagcat gtcgcagagg agtgtctggg tacgtaaaat gttcttctcc accatctggt
     4081 tgtccagctc gacctccttg ttgacgtgga tggcaaacac ttgggctaaa aattcacaag
     4141 cataacgtaa gggaaaaggg acaaatctgg gggtggtgtt gtggcagacc actaaacccc
     4201 acagcctctt tctcttctga ggctgtgtgg tggcatcggg agcatcgccc tctccatcct
     4261 cctcattcac caccacggcc atcactaaag aagcgatgct gtccatattg gccatgtatt
     4321 gcagatgaca agagtgggga gctcttaaag tgctgccgca gagggttaaa tcgaagctca
     4381 gcttctcatc ttgtaaaacc ctagcgtgct tggcgttgca gtccacgatc attctcacct
     4441 tgttcttcat aaataaaaat ctggcagctt gggggatgtc tgtggcggga tagtgtaaac
     4501 ctaaataggg ttctaaaccg ggcttggtca cttcgctcac cacctcgccg tggtcgtcct
     4561 cgtggaactt gtaggccatg acacgatcgt aaccagtcag ctcgaagact tcttgcacca
     4621 tggtgtcgca cagcctctcc atgcttccgc tgggtaaaga ctggagtctg gtgatggcct
     4681 tggcggccag cttatagctc tgtaaagctc cggcggctgt catgggcact tcatagggct
     4741 taacgggttc gaagtcgatg atgatggatc cggtcaccct atggataatg gcgtagaagg
     4801 gcttggcgga tgtacgacaa tggacgagga tagggttcag taaagacacg tcgccgaagc
     4861 cgagagcctt ctgtaaagcg gaagcgctgg gagcggtaaa taaagatctg atatcggtgc
     4921 cgatgcccag aacggggtgc tctcccacgc taggcacggc gtgggaagcc attgtgagca
     4981 gttcggaggc gttctcggag taggcgatga ccttgaaggt cttttcgtct aaagccagta
     5041 aacatccaaa gggctggatc agcttgccct tctggatatg atggaggtag gttgtggtga
     5101 ccttgtcgct cctaggaggc tggttctcga ccacgggacc agtcaccctc acgctggtgg
     5161 agtaatcaaa ggaggagccg ctctcctcga aatcggcgtg cagtttggcg tccacggtgg
     5221 tctgagcgat gatacgagcg gagtgacgag atctacgaga tccctcgctg gactgtgtgg
     5281 gacgagatcc gctcatcttc ttctcggtac cgctgccacc acttgatccg gagccagacc
     5341 ctccggaacc agacacagag atccatctct ccaccagctt ggtaaccccc agggacaggg
     5401 ccttctccac accagctgtg gacaccaggg tcttggtcct gccaatgtgg atcagcattc
     5461 tcccaccatc ggtgcgggag atgtccttca ctctgattct ggcaatttcg gcaatgcgca
     5521 gcagggtgtt gtaggcaatg cccaggaagg ccaggttcct gatgtcctgg catctgtcag
     5581 agttctccat cagggatctg acttggtcaa agtcagtgcg ttcaaaggcc agggcctgct
     5641 tggctctctc cccagcatcc acattctcct ttctgattct cctcatcacc agggacacag
     5701 cattggagtc agaagggcga ggcaggccag atctcctgtg cagcatgttg agctggccca
     5761 ggtgctgttg gatggtcttc acagccaggc ctctggcttg caggtacagg aggtagtccc
     5821 tcacatcctc aggttcagca gggaaccatt tcctgttgtt cagcttgcac caggcagccc
     5881 aggatctgca cacagacagg agcatcttcc aggtgtgttc agagaaggcc tgcctgtccc
     5941 tgaacatgtc catcaggttc ttcctgactt catcagaggt ggcatccaca gggagggcag
     6001 gcaggttttg gtgcacagtc agcaggttgg acatggtggc ggcacgcgtg cgagacgaga
     6061 cgagacagcc tgagaatgga tgcgagtaat gggcgcgcct tcgctcgaat taatcaattc
     6121 tttgccaaaa tgatgagaca gcacaataac cagcacgttg cccaggagct gtaggaaaaa
     6181 gaagaaggca tgaacatggt tagcagaggc tctagagccg ccggtcacac gccagaagcc
     6241 gaaccccgcc ctgccccgtc ccccccgaag gcagccgtcc ccccgcggac agccccgagg
     6301 ctggagaggg agaaggggac ggcggcgcgg cgacgcacga aggccctccc cgcccatttc
     6361 cttcctgccg gcgccgcacc gcttcgcccc gcgcccgcta gagggggtgc ggcggcgcct
     6421 cccagatttc ggctccgcac agatttggga caaaggaagt ccctgcgccc tctcgcacga
     6481 ttaccataaa aggcaatggc tgcggctcgc cgcgcctcga cagccgccgg cgctccgggg
     6541 gccgccgcgc ccctcccccg agccctcccc ggcccgaggc ggccccgccc cgcccggcac
     6601 ccccacctgc cgccaccccc cgcccggcac ggcgagcccc gcgccacgcc ccgtacggag
     6661 ccccgcaccc gaagccgggc cgtgctcagc aactcgggga ggggggtgca gggggggttg
     6721 cagcccgacc gacgcgccca caccccctgc tcaccccccc acgcacacac cccgcacgca
     6781 gcctttgttc ccctcgcagc cccccccgca ccgcggggca ccgcccccgg ccgcgctccc
     6841 ctcgcgcaca ctgcggagcg cacaaagccc cgcgccgcgc ccgcagcgct cacagccgcc
     6901 gggcagcgcg gagccgcacg cggcgctccc cacgcacaca cacacgcacg caccccccga
     6961 gccgctcccc ccgcacaaag ggccctcccg gagcccctca aggctttcac gcagccacag
     7021 aaaagaaaca agccgtcatt aaaccaagcg ctaattacag cccggaggag aagggccgtc
     7081 ccgcccgctc acctgtggga gtaacgcggt cagtcagagc cggggcgggc ggcgcgaggc
     7141 ggcggcggag cggggcacgg ggcgaaggca gcgcgcagcg actcccgccc gccgcgcgct
     7201 tcgcttttta tagggccgcc gccgccgccg cctcgccata aaaggaaact ttcggagcgc
     7261 gccgctctga ttggctgccg ccgcacctct ccgcctcgcc ccgccccgcc cctcgccccg
     7321 ccccgccccg cctggcgcgc gccccccccc cccccccgcc cccatcgctg cacaaaataa
     7381 ttaaaaaata aataaataca aaattggggg tggggagggg ggggagatgg ggagagtgaa
     7441 gcagaacgtg gggctcacct cgaccatggt aatagcgatg actaatacgt agatgtactg
     7501 ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc
     7561 gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg
     7621 ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac
     7681 tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg
     7741 cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga
     7801 ccccgtaatt gattactatt aataactagt caataatcaa tgt
//
