LOCUS       sequence-464995-        6225 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Red light-inducible Cre-N; Cre-N105-FHY1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Tous C, Kinstlinger IS, Rice MEL, Deng J, Wong WW
  TITLE     Multiplexing light-inducible recombinases to control cell fate, 
            Boolean logic, and cell patterning in mammalian cells.
  JOURNAL   Sci Adv. 2025 May 9;11(19):eadt1971. doi: 10.1126/sciadv.adt1971. 
            Epub 2025 May 9.
  PUBMED    40344052
REFERENCE   2  (bases 1 to 6225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-05-9"; volume: "11(19):eadt1971. doi"; pages: " 
            10.1126/sciadv.adt1971. Epub 2025 May 9"
COMMENT     Sequence Label: CT366
FEATURES             Location/Qualifiers
     source          1..6225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     26..45
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(264..368)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        399..778
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        780..1056
                     /label=chicken beta-actin promoter
     intron          1057..2073
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1997..2019
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2081..2100
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3168..3188
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3198..3422
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3662..3681)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3727..3782
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3781..3800)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4143..4159)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4143..4159)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4156..4178)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4167..4183
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4191..4197,4198..4215,4216..4221))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4236..4257
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      4342..4476
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4496..4630
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4533..4552)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4587..4606
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4698..4715)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4869..5457)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4949..4968)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5628..6225,1..194,195..263))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
       61 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
      121 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
      181 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
      241 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
      301 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
      361 gttccgcgca catttccccg aaaagtgcca cctgggtcga cattgattat tgactagtta
      421 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      481 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      541 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      601 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      661 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      721 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      781 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat
      841 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggg
      901 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
      961 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
     1021 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc
     1081 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
     1141 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
     1201 taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc
     1261 cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc
     1321 gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt
     1381 gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg
     1441 ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt
     1501 gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt tgctgagcac
     1561 ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc
     1621 ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggagggc
     1681 tcgggggagg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc ggcgagccgc
     1741 agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc
     1801 tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag
     1861 cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc
     1921 cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg
     1981 ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct
     2041 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg
     2101 ctgtctcatc attttggcaa agaattgatt aattcgagcg aaggcgcgcc cattactcgc
     2161 atccattctc aggctgtctc gtctcgtctc gcacgcgtgc cgccaccatg tccaacctgc
     2221 tgactgtgca ccaaaacctg cctgccctcc ctgtggatgc cacctctgat gaagtcagga
     2281 agaacctgat ggacatgttc agggacaggc aggccttctc tgaacacacc tggaagatgc
     2341 tcctgtctgt gtgcagatcc tgggctgcct ggtgcaagct gaacaacagg aaatggttcc
     2401 ctgctgaacc tgaggatgtg agggactacc tcctgtacct gcaagccaga ggcctggctg
     2461 tgaagaccat ccaacagcac ctgggccagc tcaacatgct gcacaggaga tctggcctgc
     2521 cttccggagg gtctggctcc ggatcaagtg gtggcagcgg tacccccgag gtggaggtcg
     2581 acaacaacaa cgagaagccc agcgagatca acagcttcca ccacatgatt atcagcagca
     2641 gcaagaacgt gctgaagatg gaggaggtgg aggtgtccaa gaagaggaag ttccagaccg
     2701 accagagcga cgagctgagc ttattacctt tatccaaaca cacatgcttt gccaacgtgg
     2761 cttgtagcga gaacaccaac ggcaacagcg agatcgatac cgagtacagc atgtccagct
     2821 acgtgaactc caccacctcc atggagtgca acaacgacat cgagatgaag gaagaatcca
     2881 gcggcagctg cggagaggac aagatgatca gcttcgagtc ccatttagac tatatctacg
     2941 gcacccagaa tctggaggac ttctccgaga aggtgatcga gaacattctg tacctcgacg
     3001 agcaagaaga ggaggaggag gacgccaagg gctgtagcag caacgctgcc aagttcgtgc
     3061 tgtcctctgg tcgttggacc gtgaaccaag atgatagcac tttacacgaa accaagaagc
     3121 ccaccatcga ccaagaattc gagcagtact ttagcacttt aatgctgccc aagaaaaagc
     3181 ggaaggtgtg agaattcctg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     3241 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     3301 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     3361 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     3421 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     3481 cgtgtaccgc tgggagttcg tagacggaaa caaacgcaga atccaagcgc ggccgcccag
     3541 gatacataga ttaccacaac tccgagccct tccaccgcta gcgcactcct caggtgcagg
     3601 ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag
     3661 atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt
     3721 ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct
     3781 cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt
     3841 agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag
     3901 gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga
     3961 cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa
     4021 aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca
     4081 tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt ggcgtaatca
     4141 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     4201 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     4261 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc
     4321 tcaattagtc agcaaccata gtcccgcccc taactccgcc cagttccgcc cattctccgc
     4381 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     4441 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt
     4501 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     4561 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     4621 tgtctggatc cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     4681 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     4741 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     4801 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     4861 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     4921 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     4981 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     5041 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     5101 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     5161 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     5221 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     5281 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     5341 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     5401 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     5461 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     5521 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     5581 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     5641 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     5701 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     5761 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     5821 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     5881 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     5941 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     6001 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     6061 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     6121 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     6181 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagt
//
