LOCUS       sequence-464996-        7236 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Red light-inducible Flp-N; Flp-N27-PhyA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7236)
  AUTHORS   Tous C, Kinstlinger IS, Rice MEL, Deng J, Wong WW
  TITLE     Multiplexing light-inducible recombinases to control cell fate, 
            Boolean logic, and cell patterning in mammalian cells.
  JOURNAL   Sci Adv. 2025 May 9;11(19):eadt1971. doi: 10.1126/sciadv.adt1971. 
            Epub 2025 May 9.
  PUBMED    40344052
REFERENCE   2  (bases 1 to 7236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-05-9"; volume: "11(19):eadt1971. doi"; pages: " 
            10.1126/sciadv.adt1971. Epub 2025 May 9"
COMMENT     Sequence Label: CT438
FEATURES             Location/Qualifiers
     source          1..7236
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        34..138
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(139..207,208..999)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(357..376)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1170..1758
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1659..1678
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1912..1929
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    1997..2131
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2021..2040)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2075..2094
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      2151..2285
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    2370..2391
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2406..2411,2412..2429,2430..2436)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2444..2460
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2449..2471
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2468..2484
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2468..2484
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2827..2846
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    2845..2900
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     2946..2965
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3205..3429
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3439..3459)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5538..5557)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          5565..6581
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(5619..5641)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(6582..6858)
                     /label=chicken beta-actin promoter
     enhancer        join(6860..7236,1..3)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtcgacccag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
       61 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
      121 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
      181 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
      241 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
      301 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
      361 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
      421 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
      481 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
      541 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
      601 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
      661 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
      721 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
      781 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
      841 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
      901 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
      961 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     1021 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     1081 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     1141 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1201 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     1261 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     1321 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     1381 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     1441 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     1501 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     1561 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     1621 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     1681 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     1741 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     1801 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     1861 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     1921 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     1981 attcattaat gcagcggatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     2041 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     2101 tgtaaccatt ataagctgca ataaacaagt tagctttttg caaaagccta ggcctccaaa
     2161 aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata
     2221 aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg
     2281 cgggactatg gttgctgact aattgagatg cggatccgct ggcacgacag gtttcccgac
     2341 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     2401 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     2461 tttcacacag gaaacagcta tgaccatgat tacgccaagc ttgggctgca ggtcgaggga
     2521 tctccataag agaagaggga cagctatgac tgggagtagt caggagagga ggaaaaatct
     2581 ggctagtaaa acatgtaagg aaaattttag ggatgttaaa gaaaaaaata acacaaaaca
     2641 aaatataaaa aaaatctaac ctcaagtcaa ggcttttcta tggaataagg aatggacagc
     2701 agggggctgt ttcatatact gatgacctct ttatagccaa cctttgttca tggcagccag
     2761 catatgggca tatgttgcca aactctaaac caaatactca ttctgatgtt ttaaatgatt
     2821 tgccctccca tatgtccttc cgagtgagag acacaaaaaa ttccaacaca ctattgcaat
     2881 gaaaataaat ttcctttatt agccagaagt cagatgctca aggggcttca tgatgtcccc
     2941 ataatttttg gcagagggaa aaagatctca gtggtatttg tgagccaggg cattggccac
     3001 accagccacc accttctgat aggcagcctg cacctgagga gtgcgctagc ggtggaaggg
     3061 ctcggagttg tggtaatcta tgtatcctgg gcggccgcgc ttggattctg cgtttgtttc
     3121 cgtctacgaa ctcccagcgg tacacgctac agggcgcgtg gggatacccc ctagagcccc
     3181 agctggttct ttccgcctca gaagccatag agcccaccgc atccccagca tgcctgctat
     3241 tgtcttccca atcctccccc ttgctgtcct gccccacccc accccccaga atagaatgac
     3301 acctactcag acaatgcgat gcaatttcct cattttatta ggaaaggaca gtgggagtgg
     3361 caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc tggcaactag
     3421 aaggcacagg aattctcaca ccttccgctt tttcttgggt tggattccat caatcttgag
     3481 atcgttgagc ttgctataga tcacctttgt gttcacgtcg gtggtctcgc tgtccttaaa
     3541 ggcgttacgt aagatgagct gtaaagagtg gatggcatcc atttcgtagt ccttccaagg
     3601 taagcttctg gtcttcacca cctccagaaa agccttgaag gaggatctgg ggtgcatcct
     3661 tctggcatcg tccctatcat cggggtcgtg tttggcgccg ccccatctca cttcgccggc
     3721 ggtatgggaa cgaaaccaga aaatcatatc tttgctgctg atacgaacgg cagccattcc
     3781 gcagacgctg tcgcctaaag ataaagccct aggaaagcca gcgtcgtgta aagaatcggt
     3841 gcttaaaccg gtggaatcca tgtggtactc acacagccaa gaagcgattt cttgtaaatg
     3901 gaactcgcta ggggtggtgc ccagcttcca gatcttgtcc ttgtataata aggcggcgcc
     3961 gtcgcacttg actaaatcca taatattggg ggactggctc acgatgccga ggggagcgtc
     4021 cctcatgagc atgtcgcaga ggagtgtctg ggtacgtaaa atgttcttct ccaccatctg
     4081 gttgtccagc tcgacctcct tgttgacgtg gatggcaaac acttgggcta aaaattcaca
     4141 agcataacgt aagggaaaag ggacaaatct gggggtggtg ttgtggcaga ccactaaacc
     4201 ccacagcctc tttctcttct gaggctgtgt ggtggcatcg ggagcatcgc cctctccatc
     4261 ctcctcattc accaccacgg ccatcactaa agaagcgatg ctgtccatat tggccatgta
     4321 ttgcagatga caagagtggg gagctcttaa agtgctgccg cagagggtta aatcgaagct
     4381 cagcttctca tcttgtaaaa ccctagcgtg cttggcgttg cagtccacga tcattctcac
     4441 cttgttcttc ataaataaaa atctggcagc ttgggggatg tctgtggcgg gatagtgtaa
     4501 acctaaatag ggttctaaac cgggcttggt cacttcgctc accacctcgc cgtggtcgtc
     4561 ctcgtggaac ttgtaggcca tgacacgatc gtaaccagtc agctcgaaga cttcttgcac
     4621 catggtgtcg cacagcctct ccatgcttcc gctgggtaaa gactggagtc tggtgatggc
     4681 cttggcggcc agcttatagc tctgtaaagc tccggcggct gtcatgggca cttcataggg
     4741 cttaacgggt tcgaagtcga tgatgatgga tccggtcacc ctatggataa tggcgtagaa
     4801 gggcttggcg gatgtacgac aatggacgag gatagggttc agtaaagaca cgtcgccgaa
     4861 gccgagagcc ttctgtaaag cggaagcgct gggagcggta aataaagatc tgatatcggt
     4921 gccgatgccc agaacggggt gctctcccac gctaggcacg gcgtgggaag ccattgtgag
     4981 cagttcggag gcgttctcgg agtaggcgat gaccttgaag gtcttttcgt ctaaagccag
     5041 taaacatcca aagggctgga tcagcttgcc cttctggata tgatggaggt aggttgtggt
     5101 gaccttgtcg ctcctaggag gctggttctc gaccacggga ccagtcaccc tcacgctggt
     5161 ggagtaatca aaggaggagc cgctctcctc gaaatcggcg tgcagtttgg cgtccacggt
     5221 ggtctgagcg atgatacgag cggagtgacg agatctacga gatccctcgc tggactgtgt
     5281 gggacgagat ccgctcatct tcttctcggt accgctgcca ccacttgatc cggagccaga
     5341 ccctccggag ctgggcctct cgaatctctc cacgaactgc cgcaccagca ccttgggggg
     5401 ggtcttgcac aggatgtcga actggctcat ggtggcggca cgcgtgcgag acgagacgag
     5461 acagcctgag aatggatgcg agtaatgggc gcgccttcgc tcgaattaat caattctttg
     5521 ccaaaatgat gagacagcac aataaccagc acgttgccca ggagctgtag gaaaaagaag
     5581 aaggcatgaa catggttagc agaggctcta gagccgccgg tcacacgcca gaagccgaac
     5641 cccgccctgc cccgtccccc ccgaaggcag ccgtcccccc gcggacagcc ccgaggctgg
     5701 agagggagaa ggggacggcg gcgcggcgac gcacgaaggc cctccccgcc catttccttc
     5761 ctgccggcgc cgcaccgctt cgccccgcgc ccgctagagg gggtgcggcg gcgcctccca
     5821 gatttcggct ccgcacagat ttgggacaaa ggaagtccct gcgccctctc gcacgattac
     5881 cataaaaggc aatggctgcg gctcgccgcg cctcgacagc cgccggcgct ccgggggccg
     5941 ccgcgcccct cccccgagcc ctccccggcc cgaggcggcc ccgccccgcc cggcaccccc
     6001 acctgccgcc accccccgcc cggcacggcg agccccgcgc cacgccccgt acggagcccc
     6061 gcacccgaag ccgggccgtg ctcagcaact cggggagggg ggtgcagggg gggttgcagc
     6121 ccgaccgacg cgcccacacc ccctgctcac ccccccacgc acacaccccg cacgcagcct
     6181 ttgttcccct cgcagccccc cccgcaccgc ggggcaccgc ccccggccgc gctcccctcg
     6241 cgcacactgc ggagcgcaca aagccccgcg ccgcgcccgc agcgctcaca gccgccgggc
     6301 agcgcggagc cgcacgcggc gctccccacg cacacacaca cgcacgcacc ccccgagccg
     6361 ctccccccgc acaaagggcc ctcccggagc ccctcaaggc tttcacgcag ccacagaaaa
     6421 gaaacaagcc gtcattaaac caagcgctaa ttacagcccg gaggagaagg gccgtcccgc
     6481 ccgctcacct gtgggagtaa cgcggtcagt cagagccggg gcgggcggcg cgaggcggcg
     6541 gcggagcggg gcacggggcg aaggcagcgc gcagcgactc ccgcccgccg cgcgcttcgc
     6601 tttttatagg gccgccgccg ccgccgcctc gccataaaag gaaactttcg gagcgcgccg
     6661 ctctgattgg ctgccgccgc acctctccgc ctcgccccgc cccgcccctc gccccgcccc
     6721 gccccgcctg gcgcgcgccc cccccccccc ccgcccccat cgctgcacaa aataattaaa
     6781 aaataaataa atacaaaatt gggggtgggg agggggggga gatggggaga gtgaagcaga
     6841 acgtggggct cacctcgacc atggtaatag cgatgactaa tacgtagatg tactgccaag
     6901 taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat
     6961 tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag
     7021 tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg
     7081 gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc
     7141 catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg
     7201 taattgatta ctattaataa ctagtcaata atcaat
//
