LOCUS       sequence-464997-        7098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Red light-inducible Flp-N; Flp-N396-FHY1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7098)
  AUTHORS   Tous C, Kinstlinger IS, Rice MEL, Deng J, Wong WW
  TITLE     Multiplexing light-inducible recombinases to control cell fate, 
            Boolean logic, and cell patterning in mammalian cells.
  JOURNAL   Sci Adv. 2025 May 9;11(19):eadt1971. doi: 10.1126/sciadv.adt1971. 
            Epub 2025 May 9.
  PUBMED    40344052
REFERENCE   2  (bases 1 to 7098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-05-9"; volume: "11(19):eadt1971. doi"; pages: " 
            10.1126/sciadv.adt1971. Epub 2025 May 9"
COMMENT     Sequence Label: CT452
FEATURES             Location/Qualifiers
     source          1..7098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(115..134)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      928..1516
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1417..1436
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1670..1687
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    1755..1889
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1779..1798)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1833..1852
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      1909..2043
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    2128..2149
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2164..2169,2170..2187,2188..2194)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2202..2218
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2207..2229
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2226..2242
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2226..2242
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2585..2604
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    2603..2658
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     2704..2723
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2963..3187
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3197..3217)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5158..5177)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          5185..6201
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(5239..5261)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(6202..6478)
                     /label=chicken beta-actin promoter
     enhancer        6480..6859
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6890..6994
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6995..7063,7064..7098,1..757)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
       61 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      121 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      181 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      241 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
      301 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
      361 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
      421 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
      481 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
      541 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
      601 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
      661 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
      721 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
      781 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
      841 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
      901 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
      961 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1021 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     1081 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1141 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1201 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1261 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1321 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1381 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1441 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1501 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1561 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     1621 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     1681 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     1741 tcattaatgc agcggatcca gacatgataa gatacattga tgagtttgga caaaccacaa
     1801 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     1861 taaccattat aagctgcaat aaacaagtta gctttttgca aaagcctagg cctccaaaaa
     1921 agcctcctca ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa
     1981 taaaaaaaat tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg
     2041 ggactatggt tgctgactaa ttgagatgcg gatccgctgg cacgacaggt ttcccgactg
     2101 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     2161 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     2221 tcacacagga aacagctatg accatgatta cgccaagctt gggctgcagg tcgagggatc
     2281 tccataagag aagagggaca gctatgactg ggagtagtca ggagaggagg aaaaatctgg
     2341 ctagtaaaac atgtaaggaa aattttaggg atgttaaaga aaaaaataac acaaaacaaa
     2401 atataaaaaa aatctaacct caagtcaagg cttttctatg gaataaggaa tggacagcag
     2461 ggggctgttt catatactga tgacctcttt atagccaacc tttgttcatg gcagccagca
     2521 tatgggcata tgttgccaaa ctctaaacca aatactcatt ctgatgtttt aaatgatttg
     2581 ccctcccata tgtccttccg agtgagagac acaaaaaatt ccaacacact attgcaatga
     2641 aaataaattt cctttattag ccagaagtca gatgctcaag gggcttcatg atgtccccat
     2701 aatttttggc agagggaaaa agatctcagt ggtatttgtg agccagggca ttggccacac
     2761 cagccaccac cttctgatag gcagcctgca cctgaggagt gcgctagcgg tggaagggct
     2821 cggagttgtg gtaatctatg tatcctgggc ggccgcgctt ggattctgcg tttgtttccg
     2881 tctacgaact cccagcggta cacgctacag ggcgcgtggg gataccccct agagccccag
     2941 ctggttcttt ccgcctcaga agccatagag cccaccgcat ccccagcatg cctgctattg
     3001 tcttcccaat cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac
     3061 ctactcagac aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca
     3121 ccttccaggg tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa
     3181 ggcacaggaa ttctcacacc ttccgctttt tcttgggcag cattaaagtg ctaaagtact
     3241 gctcgaattc ttggtcgatg gtgggcttct tggtttcgtg taaagtgcta tcatcttggt
     3301 tcacggtcca acgaccagag gacagcacga acttggcagc gttgctgcta cagcccttgg
     3361 cgtcctcctc ctcctcttct tgctcgtcga ggtacagaat gttctcgatc accttctcgg
     3421 agaagtcctc cagattctgg gtgccgtaga tatagtctaa atgggactcg aagctgatca
     3481 tcttgtcctc tccgcagctg ccgctggatt cttccttcat ctcgatgtcg ttgttgcact
     3541 ccatggaggt ggtggagttc acgtagctgg acatgctgta ctcggtatcg atctcgctgt
     3601 tgccgttggt gttctcgcta caagccacgt tggcaaagca tgtgtgtttg gataaaggta
     3661 ataagctcag ctcgtcgctc tggtcggtct ggaacttcct cttcttggac acctccacct
     3721 cctccatctt cagcacgttc ttgctgctgc tgataatcat gtggtggaag ctgttgatct
     3781 cgctgggctt ctcgttgttg ttgtcgacct ccacctcggg ggtaccgctg ccaccacttg
     3841 atccggagcc agaccctccg gagccctcgg cgctgccctt cagctgctcg atgtgctgcc
     3901 actcctcgat ggggttggtc tcgtccttca gggcgatcat ctccttgctg atggggtcgt
     3961 aggcgtagta cctggacacc agggcgaagt agtggtcggg gatggcggtg atctggtggg
     4021 tgtaggtggt cctggccacg gcggaggccc tcttgtcgct ccagttgccc accacgtttg
     4081 tcagctcggt caggcccttc atgctcagaa agctggtcat caggtgcctg ccgatgtggc
     4141 tcttagggcc gttcttgata gcgaagatgg ggtagggggc gttcttcttc agggccttgt
     4201 tgtagctgcg caccaggttg tccttcagca gctggtactc ctgcttgttg ctgctgctgt
     4261 tgccggtcct gttcactctc ttcagcacgg gctcgctgtt cctcaggaac tcgtccaggt
     4321 acaccagggg gtcgatcctg cctctggcgc tgaaaaagta gatgtgcctg gacacgcttg
     4381 tcttggtctc ggtcaccagg cactgaatga tcacgcccag gtacttgttc tgcaccagct
     4441 tgaagctctt ggggtccacg ttcttgatgt cgctgaacct gccgcagttg atgaatgtgg
     4501 ccaggaacag gaactggtac agggtcttgg tcttggtgaa cctgctggtg tactcgaagc
     4561 tgttcaggat cttctcggtg atctcccaga tgctctcgcc ctcggacagc agggccttca
     4621 gcatcttctt gctgtggctg ttgcccttgt cggcctcctc gctgctctcg aactgcagct
     4681 gcaggctgga cacgatgtcg gtgatgtcgc tctggtgctt ctggccgttg taagggatga
     4741 tggtgaactc ccaggcgggg atcagcttct tcaggctggc ctccaggatg gtggccttct
     4801 gggtcttgta cttgaactgc aggctcttgt tcacgatgtc gaagctcagg ctgttgctga
     4861 tgatggtgtt gtagctcatg aaggtggccc tcttgatggc ggtgccgttg tgggtgatca
     4921 tccagcacag gtaggtcagc tcggcggcac agctggcgat cttctcgccg ctgggcctct
     4981 cgaatctctc cacgaactgc cgcaccagca ccttgggggg ggtcttgcac aggatgtcga
     5041 actggctcat ggtggcggca cgcgtgcgag acgagacgag acagcctgag aatggatgcg
     5101 agtaatgggc gcgccttcgc tcgaattaat caattctttg ccaaaatgat gagacagcac
     5161 aataaccagc acgttgccca ggagctgtag gaaaaagaag aaggcatgaa catggttagc
     5221 agaggctcta gagccgccgg tcacacgcca gaagccgaac cccgccctgc cccgtccccc
     5281 ccgaaggcag ccgtcccccc gcggacagcc ccgaggctgg agagggagaa ggggacggcg
     5341 gcgcggcgac gcacgaaggc cctccccgcc catttccttc ctgccggcgc cgcaccgctt
     5401 cgccccgcgc ccgctagagg gggtgcggcg gcgcctccca gatttcggct ccgcacagat
     5461 ttgggacaaa ggaagtccct gcgccctctc gcacgattac cataaaaggc aatggctgcg
     5521 gctcgccgcg cctcgacagc cgccggcgct ccgggggccg ccgcgcccct cccccgagcc
     5581 ctccccggcc cgaggcggcc ccgccccgcc cggcaccccc acctgccgcc accccccgcc
     5641 cggcacggcg agccccgcgc cacgccccgt acggagcccc gcacccgaag ccgggccgtg
     5701 ctcagcaact cggggagggg ggtgcagggg gggttgcagc ccgaccgacg cgcccacacc
     5761 ccctgctcac ccccccacgc acacaccccg cacgcagcct ttgttcccct cgcagccccc
     5821 cccgcaccgc ggggcaccgc ccccggccgc gctcccctcg cgcacactgc ggagcgcaca
     5881 aagccccgcg ccgcgcccgc agcgctcaca gccgccgggc agcgcggagc cgcacgcggc
     5941 gctccccacg cacacacaca cgcacgcacc ccccgagccg ctccccccgc acaaagggcc
     6001 ctcccggagc ccctcaaggc tttcacgcag ccacagaaaa gaaacaagcc gtcattaaac
     6061 caagcgctaa ttacagcccg gaggagaagg gccgtcccgc ccgctcacct gtgggagtaa
     6121 cgcggtcagt cagagccggg gcgggcggcg cgaggcggcg gcggagcggg gcacggggcg
     6181 aaggcagcgc gcagcgactc ccgcccgccg cgcgcttcgc tttttatagg gccgccgccg
     6241 ccgccgcctc gccataaaag gaaactttcg gagcgcgccg ctctgattgg ctgccgccgc
     6301 acctctccgc ctcgccccgc cccgcccctc gccccgcccc gccccgcctg gcgcgcgccc
     6361 cccccccccc ccgcccccat cgctgcacaa aataattaaa aaataaataa atacaaaatt
     6421 gggggtgggg agggggggga gatggggaga gtgaagcaga acgtggggct cacctcgacc
     6481 atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc
     6541 atgtactggg cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta
     6601 cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc
     6661 cattgacgtc aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac
     6721 gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta
     6781 acgcggaact ccatatatgg gctatgaact aatgaccccg taattgatta ctattaataa
     6841 ctagtcaata atcaatgtcg acccaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     6901 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     6961 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     7021 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     7081 gaaagtaaaa gatgctga
//
