LOCUS       sequence-464998-        7101 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Red light-inducible Flp-C; Flp-28C-FHY1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7101)
  AUTHORS   Tous C, Kinstlinger IS, Rice MEL, Deng J, Wong WW
  TITLE     Multiplexing light-inducible recombinases to control cell fate, 
            Boolean logic, and cell patterning in mammalian cells.
  JOURNAL   Sci Adv. 2025 May 9;11(19):eadt1971. doi: 10.1126/sciadv.adt1971. 
            Epub 2025 May 9.
  PUBMED    40344052
REFERENCE   2  (bases 1 to 7101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-05-9"; volume: "11(19):eadt1971. doi"; pages: " 
            10.1126/sciadv.adt1971. Epub 2025 May 9"
COMMENT     Sequence Label: CT447
FEATURES             Location/Qualifiers
     source          1..7101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    75..96
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(111..116,117..134,135..141)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    149..165
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     154..176
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     173..189
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     173..189
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     532..551
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    550..605
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     651..670
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    910..1134
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(1144..1164)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3108..3127)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          3135..4151
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(3189..3211)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(4152..4428)
                     /label=chicken beta-actin promoter
     enhancer        4430..4809
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4840..4944
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4945..5013,5014..5805)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5163..5182)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5976..6564
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6465..6484
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6718..6735
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    6803..6937
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6827..6846)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6881..6900
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      6957..7091
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 tgactaattg agatgcggat ccgctggcac gacaggtttc ccgactggaa agcgggcagt
       61 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
      121 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
      181 agctatgacc atgattacgc caagcttggg ctgcaggtcg agggatctcc ataagagaag
      241 agggacagct atgactggga gtagtcagga gaggaggaaa aatctggcta gtaaaacatg
      301 taaggaaaat tttagggatg ttaaagaaaa aaataacaca aaacaaaata taaaaaaaat
      361 ctaacctcaa gtcaaggctt ttctatggaa taaggaatgg acagcagggg gctgtttcat
      421 atactgatga cctctttata gccaaccttt gttcatggca gccagcatat gggcatatgt
      481 tgccaaactc taaaccaaat actcattctg atgttttaaa tgatttgccc tcccatatgt
      541 ccttccgagt gagagacaca aaaaattcca acacactatt gcaatgaaaa taaatttcct
      601 ttattagcca gaagtcagat gctcaagggg cttcatgatg tccccataat ttttggcaga
      661 gggaaaaaga tctcagtggt atttgtgagc cagggcattg gccacaccag ccaccacctt
      721 ctgataggca gcctgcacct gaggagtgcg ctagcggtgg aagggctcgg agttgtggta
      781 atctatgtat cctgggcggc cgcgcttgga ttctgcgttt gtttccgtct acgaactccc
      841 agcggtacac gctacagggc gcgtggggat accccctaga gccccagctg gttctttccg
      901 cctcagaagc catagagccc accgcatccc cagcatgcct gctattgtct tcccaatcct
      961 cccccttgct gtcctgcccc accccacccc ccagaataga atgacaccta ctcagacaat
     1021 gcgatgcaat ttcctcattt tattaggaaa ggacagtggg agtggcacct tccagggtca
     1081 aggaaggcac gggggagggg caaacaacag atggctggca actagaaggc acaggaattc
     1141 tcacaccttc cgctttttct tggggatccg cctgttgatg tagctgctca ggtagtccag
     1201 cacctcctgg ctgatgatgc cgttccaggc ggggtatctg atgctgccct cggcgctgcc
     1261 cttcagctgc tcgatgtgct gccactcctc gatggggttg gtctcgtcct tcagggcgat
     1321 catctccttg ctgatggggt cgtaggcgta gtacctggac accagggcga agtagtggtc
     1381 ggggatggcg gtgatctggt gggtgtaggt ggtcctggcc acggcggagg ccctcttgtc
     1441 gctccagttg cccaccacgt ttgtcagctc ggtcaggccc ttcatgctca gaaagctggt
     1501 catcaggtgc ctgccgatgt ggctcttagg gccgttcttg atagcgaaga tggggtaggg
     1561 ggcgttcttc ttcagggcct tgttgtagct gcgcaccagg ttgtccttca gcagctggta
     1621 ctcctgcttg ttgctgctgc tgttgccggt cctgttcact ctcttcagca cgggctcgct
     1681 gttcctcagg aactcgtcca ggtacaccag ggggtcgatc ctgcctctgg cgctgaaaaa
     1741 gtagatgtgc ctggacacgc ttgtcttggt ctcggtcacc aggcactgaa tgatcacgcc
     1801 caggtacttg ttctgcacca gcttgaagct cttggggtcc acgttcttga tgtcgctgaa
     1861 cctgccgcag ttgatgaatg tggccaggaa caggaactgg tacagggtct tggtcttggt
     1921 gaacctgctg gtgtactcga agctgttcag gatcttctcg gtgatctccc agatgctctc
     1981 gccctcggac agcagggcct tcagcatctt cttgctgtgg ctgttgccct tgtcggcctc
     2041 ctcgctgctc tcgaactgca gctgcaggct ggacacgatg tcggtgatgt cgctctggtg
     2101 cttctggccg ttgtaaggga tgatggtgaa ctcccaggcg gggatcagct tcttcaggct
     2161 ggcctccagg atggtggcct tctgggtctt gtacttgaac tgcaggctct tgttcacgat
     2221 gtcgaagctc aggctgttgc tgatgatggt gttgtagctc atgaaggtgg ccctcttgat
     2281 ggcggtgccg ttgtgggtga tcatccagca caggtaggtc agctcggcgg cacagctggc
     2341 gatcttctcg ccggtaccgc tgccaccact tgatccggag ccagaccctc cggacagcat
     2401 taaagtgcta aagtactgct cgaattcttg gtcgatggtg ggcttcttgg tttcgtgtaa
     2461 agtgctatca tcttggttca cggtccaacg accagaggac agcacgaact tggcagcgtt
     2521 gctgctacag cccttggcgt cctcctcctc ctcttcttgc tcgtcgaggt acagaatgtt
     2581 ctcgatcacc ttctcggaga agtcctccag attctgggtg ccgtagatat agtctaaatg
     2641 ggactcgaag ctgatcatct tgtcctctcc gcagctgccg ctggattctt ccttcatctc
     2701 gatgtcgttg ttgcactcca tggaggtggt ggagttcacg tagctggaca tgctgtactc
     2761 ggtatcgatc tcgctgttgc cgttggtgtt ctcgctacaa gccacgttgg caaagcatgt
     2821 gtgtttggat aaaggtaata agctcagctc gtcgctctgg tcggtctgga acttcctctt
     2881 cttggacacc tccacctcct ccatcttcag cacgttcttg ctgctgctga taatcatgtg
     2941 gtggaagctg ttgatctcgc tgggcttctc gttgttgttg tcgacctcca cctcgggcat
     3001 ggtggcggca cgcgtgcgag acgagacgag acagcctgag aatggatgcg agtaatgggc
     3061 gcgccttcgc tcgaattaat caattctttg ccaaaatgat gagacagcac aataaccagc
     3121 acgttgccca ggagctgtag gaaaaagaag aaggcatgaa catggttagc agaggctcta
     3181 gagccgccgg tcacacgcca gaagccgaac cccgccctgc cccgtccccc ccgaaggcag
     3241 ccgtcccccc gcggacagcc ccgaggctgg agagggagaa ggggacggcg gcgcggcgac
     3301 gcacgaaggc cctccccgcc catttccttc ctgccggcgc cgcaccgctt cgccccgcgc
     3361 ccgctagagg gggtgcggcg gcgcctccca gatttcggct ccgcacagat ttgggacaaa
     3421 ggaagtccct gcgccctctc gcacgattac cataaaaggc aatggctgcg gctcgccgcg
     3481 cctcgacagc cgccggcgct ccgggggccg ccgcgcccct cccccgagcc ctccccggcc
     3541 cgaggcggcc ccgccccgcc cggcaccccc acctgccgcc accccccgcc cggcacggcg
     3601 agccccgcgc cacgccccgt acggagcccc gcacccgaag ccgggccgtg ctcagcaact
     3661 cggggagggg ggtgcagggg gggttgcagc ccgaccgacg cgcccacacc ccctgctcac
     3721 ccccccacgc acacaccccg cacgcagcct ttgttcccct cgcagccccc cccgcaccgc
     3781 ggggcaccgc ccccggccgc gctcccctcg cgcacactgc ggagcgcaca aagccccgcg
     3841 ccgcgcccgc agcgctcaca gccgccgggc agcgcggagc cgcacgcggc gctccccacg
     3901 cacacacaca cgcacgcacc ccccgagccg ctccccccgc acaaagggcc ctcccggagc
     3961 ccctcaaggc tttcacgcag ccacagaaaa gaaacaagcc gtcattaaac caagcgctaa
     4021 ttacagcccg gaggagaagg gccgtcccgc ccgctcacct gtgggagtaa cgcggtcagt
     4081 cagagccggg gcgggcggcg cgaggcggcg gcggagcggg gcacggggcg aaggcagcgc
     4141 gcagcgactc ccgcccgccg cgcgcttcgc tttttatagg gccgccgccg ccgccgcctc
     4201 gccataaaag gaaactttcg gagcgcgccg ctctgattgg ctgccgccgc acctctccgc
     4261 ctcgccccgc cccgcccctc gccccgcccc gccccgcctg gcgcgcgccc cccccccccc
     4321 ccgcccccat cgctgcacaa aataattaaa aaataaataa atacaaaatt gggggtgggg
     4381 agggggggga gatggggaga gtgaagcaga acgtggggct cacctcgacc atggtaatag
     4441 cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg
     4501 cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat
     4561 gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc
     4621 aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc
     4681 gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact
     4741 ccatatatgg gctatgaact aatgaccccg taattgatta ctattaataa ctagtcaata
     4801 atcaatgtcg acccaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     4861 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     4921 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     4981 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     5041 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5101 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5161 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     5221 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     5281 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     5341 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     5401 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     5461 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     5521 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     5581 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     5641 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     5701 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     5761 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     5821 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5881 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5941 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6001 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6061 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6121 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6181 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6241 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6301 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     6361 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     6421 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     6481 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     6541 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     6601 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     6661 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     6721 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     6781 tggccgattc attaatgcag cggatccaga catgataaga tacattgatg agtttggaca
     6841 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     6901 tttatttgta accattataa gctgcaataa acaagttagc tttttgcaaa agcctaggcc
     6961 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc
     7021 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
     7081 taggggcggg actatggttg c
//
