LOCUS       sequence-465000-        7012 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Red light-inducible Flp-N; Flp-N374-FHY1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7012)
  AUTHORS   Tous C, Kinstlinger IS, Rice MEL, Deng J, Wong WW
  TITLE     Multiplexing light-inducible recombinases to control cell fate, 
            Boolean logic, and cell patterning in mammalian cells.
  JOURNAL   Sci Adv. 2025 May 9;11(19):eadt1971. doi: 10.1126/sciadv.adt1971. 
            Epub 2025 May 9.
  PUBMED    40344052
REFERENCE   2  (bases 1 to 7012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-05-9"; volume: "11(19):eadt1971. doi"; pages: " 
            10.1126/sciadv.adt1971. Epub 2025 May 9"
COMMENT     Sequence Label: CT450
FEATURES             Location/Qualifiers
     source          1..7012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     22..41
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(260..364)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        395..774
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        776..1032
                     /label=chicken beta-actin promoter
                     /note="contains a 21-bp deletion relative to the standard 
                     chicken beta-actin promoter"
     intron          1033..2049
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1973..1995
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2057..2076
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3951..3971
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3981..4205
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4445..4464)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4510..4565
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4564..4583)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4926..4942)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4926..4942)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4939..4961)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4950..4966
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4974..4980,4981..4998,4999..5004))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5019..5040
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      5125..5259
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5279..5413
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5316..5335)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5370..5389
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5481..5498)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5652..6240)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5732..5751)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6411..7012,1..190,191..259))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
       61 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      121 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      181 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      241 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
      301 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
      361 cgcgcacatt tccccgaaaa gtgccacctg ggtcgacatt gattattgac tagttattaa
      421 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
      481 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
      541 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
      601 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
      661 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
      721 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag
      781 gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg
      841 tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg ggcggggcgg
      901 ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg
      961 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
     1021 gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc
     1081 tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac
     1141 ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg
     1201 tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg
     1261 gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc
     1321 ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg
     1381 agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc
     1441 gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa
     1501 ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc
     1561 cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg
     1621 ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc
     1681 cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg
     1741 tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg
     1801 cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa
     1861 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct
     1921 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
     1981 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt
     2041 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
     2101 ttgattaatt cgagcgaagg cgcgcccatt actcgcatcc attctcaggc tgtctcgtct
     2161 cgtctcgcac gcgtgccgcc accatgagcc agttcgacat cctgtgcaag acccccccca
     2221 aggtgctggt gcggcagttc gtggagagat tcgagaggcc cagcggcgag aagatcgcca
     2281 gctgtgccgc cgagctgacc tacctgtgct ggatgatcac ccacaacggc accgccatca
     2341 agagggccac cttcatgagc tacaacacca tcatcagcaa cagcctgagc ttcgacatcg
     2401 tgaacaagag cctgcagttc aagtacaaga cccagaaggc caccatcctg gaggccagcc
     2461 tgaagaagct gatccccgcc tgggagttca ccatcatccc ttacaacggc cagaagcacc
     2521 agagcgacat caccgacatc gtgtccagcc tgcagctgca gttcgagagc agcgaggagg
     2581 ccgacaaggg caacagccac agcaagaaga tgctgaaggc cctgctgtcc gagggcgaga
     2641 gcatctggga gatcaccgag aagatcctga acagcttcga gtacaccagc aggttcacca
     2701 agaccaagac cctgtaccag ttcctgttcc tggccacatt catcaactgc ggcaggttca
     2761 gcgacatcaa gaacgtggac cccaagagct tcaagctggt gcagaacaag tacctgggcg
     2821 tgatcattca gtgcctggtg accgagacca agacaagcgt gtccaggcac atctactttt
     2881 tcagcgccag aggcaggatc gaccccctgg tgtacctgga cgagttcctg aggaacagcg
     2941 agcccgtgct gaagagagtg aacaggaccg gcaacagcag cagcaacaag caggagtacc
     3001 agctgctgaa ggacaacctg gtgcgcagct acaacaaggc cctgaagaag aacgccccct
     3061 accccatctt cgctatcaag aacggcccta agagccacat cggcaggcac ctgatgacca
     3121 gctttctgag catgaagggc ctgaccgagc tgacaaacgt ggtgggcaac tggagcgaca
     3181 agagggcctc cgccgtggcc aggaccacct acacccacca gatcaccgcc atccccgacc
     3241 actacttcgc cctggtgtcc aggtactacg cctacgaccc catcagcaag gagatgatcg
     3301 ccctgtccgg agggtctggc tccggatcaa gtggtggcag cggtaccccc gaggtggagg
     3361 tcgacaacaa caacgagaag cccagcgaga tcaacagctt ccaccacatg attatcagca
     3421 gcagcaagaa cgtgctgaag atggaggagg tggaggtgtc caagaagagg aagttccaga
     3481 ccgaccagag cgacgagctg agcttattac ctttatccaa acacacatgc tttgccaacg
     3541 tggcttgtag cgagaacacc aacggcaaca gcgagatcga taccgagtac agcatgtcca
     3601 gctacgtgaa ctccaccacc tccatggagt gcaacaacga catcgagatg aaggaagaat
     3661 ccagcggcag ctgcggagag gacaagatga tcagcttcga gtcccattta gactatatct
     3721 acggcaccca gaatctggag gacttctccg agaaggtgat cgagaacatt ctgtacctcg
     3781 acgagcaaga agaggaggag gaggacgcca agggctgtag cagcaacgct gccaagttcg
     3841 tgctgtcctc tggtcgttgg accgtgaacc aagatgatag cactttacac gaaaccaaga
     3901 agcccaccat cgaccaagaa ttcgagcagt actttagcac tttaatgctg cccaagaaaa
     3961 agcggaaggt gtgagaattc ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     4021 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     4081 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     4141 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     4201 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     4261 tagcgtgtac cgctgggagt tcgtagacgg aaacaaacgc agaatccaag cgcggccgcc
     4321 caggatacat agattaccac aactccgagc ccttccaccg ctagcgcact cctcaggtgc
     4381 aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact
     4441 gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga
     4501 cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc
     4561 tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg
     4621 tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt tggctataaa
     4681 gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct
     4741 tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc
     4801 taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag
     4861 tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag cttggcgtaa
     4921 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     4981 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     5041 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc
     5101 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccagttcc gcccattctc
     5161 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     5221 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctaa
     5281 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     5341 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     5401 tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     5461 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     5521 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     5581 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     5641 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     5701 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     5761 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     5821 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     5881 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     5941 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     6001 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     6061 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     6121 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     6181 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     6241 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     6301 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     6361 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     6421 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     6481 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     6541 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     6601 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     6661 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     6721 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     6781 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     6841 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     6901 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     6961 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ct
//
