LOCUS       sequence-465001-        7307 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Red light-inducible Flp-C; Flp-375C-PhyA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7307)
  AUTHORS   Tous C, Kinstlinger IS, Rice MEL, Deng J, Wong WW
  TITLE     Multiplexing light-inducible recombinases to control cell fate, 
            Boolean logic, and cell patterning in mammalian cells.
  JOURNAL   Sci Adv. 2025 May 9;11(19):eadt1971. doi: 10.1126/sciadv.adt1971. 
            Epub 2025 May 9.
  PUBMED    40344052
REFERENCE   2  (bases 1 to 7307)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-05-9"; volume: "11(19):eadt1971. doi"; pages: " 
            10.1126/sciadv.adt1971. Epub 2025 May 9"
COMMENT     Sequence Label: CT443
FEATURES             Location/Qualifiers
     source          1..7307
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(104..123)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      917..1505
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1406..1425
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1659..1676
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    1744..1878
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1768..1787)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1822..1841
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      1898..2032
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    2117..2138
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2153..2158,2159..2176,2177..2183)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2191..2207
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2196..2218
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2215..2231
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2215..2231
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2574..2593
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    2592..2647
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     2693..2712
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2952..3176
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3186..3206)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      5243..5252
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5354..5373)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          5381..6397
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(5435..5457)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(6398..6676)
                     /label=chicken beta-actin promoter
     enhancer        6678..7057
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7088..7192
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7193..7261,7262..7307,1..746)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
       61 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      121 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      181 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      241 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      301 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      361 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      421 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      481 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      541 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      601 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      661 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      721 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
      781 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
      841 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
      901 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      961 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1021 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1081 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1141 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1201 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1261 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1321 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1381 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1441 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1501 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1561 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     1621 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     1681 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     1741 gcggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     1801 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     1861 agctgcaata aacaagttag ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac
     1921 tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt
     1981 agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gactatggtt
     2041 gctgactaat tgagatgcgg atccgctggc acgacaggtt tcccgactgg aaagcgggca
     2101 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     2161 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     2221 acagctatga ccatgattac gccaagcttg ggctgcaggt cgagggatct ccataagaga
     2281 agagggacag ctatgactgg gagtagtcag gagaggagga aaaatctggc tagtaaaaca
     2341 tgtaagaaaa attttaggga tgttaaagaa aaaaataaca caaaacaaaa tataaaaaaa
     2401 atctaacctc aagtcaaggc ttttctatgg aataaggaat ggacagcagg gggctgtttc
     2461 atatactgat gacctcttta tagccaacct ttgttcatgg cagccagcat atgggcatat
     2521 gttgccaaac tctaaaccaa atactcattc tgatgtttta aatgatttgc cctcccatat
     2581 gtccttccga gtgagagaca caaaaaattc caacacacta ttgcaatgaa aataaatttc
     2641 ctttattagc cagaagtcag atgctcaagg ggcttcatga tgtccccata atttttggca
     2701 gagggaaaaa gatctcagtg gtatttgtga gccagggcat tggccacacc agccaccacc
     2761 ttctgatagg cagcctgcac ctgaggagtg cgctagcggt ggaagggctc ggagttgtgg
     2821 taatctatgt atcctgggcg gccgcgcttg gattctgcgt ttgtttccgt ctacgaactc
     2881 ccagcggtac acgctacagg gcgcgtgggg atacccccta gagccccagc tggttctttc
     2941 cgcctcagaa gccatagagc ccaccgcatc cccagcatgc ctgctattgt cttcccaatc
     3001 ctcccccttg ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca
     3061 atgcgatgca atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt
     3121 caaggaaggc acgggggagg ggcaaacaac agatggctgg caactagaag gcacaggaat
     3181 tctcacacct tccgcttttt cttggggatc cgcctgttga tgtagctgct caggtagtcc
     3241 agcacctcct ggctgatgat gccgttccag gcggggtatc tgatgctgcc ctcggcgctg
     3301 cccttcagct gctcgatgtg ctgccactcc tcgatggggt tggtctcgtc cttggtaccg
     3361 ctgccaccac ttgatccgga gccagaccct ccggattgga ttccatcaat cttgagatcg
     3421 ttgagcttgc tatagatcac ctttgtgttc acgtcggtgg tctcgctgtc cttaaaggcg
     3481 ttacgtaaga tgagctgtaa agagtggatg gcatccattt cgtagtcctt ccaaggtaag
     3541 cttctggtct tcaccacctc cagaaaagcc ttgaaggagg atctggggtg catccttctg
     3601 gcatcgtccc tatcatcggg gtcgtgtttg gcgccgcccc atctcacttc gccggcggta
     3661 tgggaacgaa accagaaaat catatctttg ctgctgatac gaacggcagc cattccgcag
     3721 acgctgtcgc ctaaagataa agccctagga aagccagcgt cgtgtaaaga atcggtgctt
     3781 aaaccggtgg aatccatgtg gtactcacac agccaagaag cgatttcttg taaatggaac
     3841 tcgctagggg tggtgcccag cttccagatc ttgtccttgt ataataaggc ggcgccgtcg
     3901 cacttgacta aatccataat attgggggac tggctcacga tgccgagggg agcgtccctc
     3961 atgagcatgt cgcagaggag tgtctgggta cgtaaaatgt tcttctccac catctggttg
     4021 tccagctcga cctccttgtt gacgtggatg gcaaacactt gggctaaaaa ttcacaagca
     4081 taacgtaagg gaaaagggac aaatctgggg gtggtgttgt ggcagaccac taaaccccac
     4141 agcctctttc tcttctgagg ctgtgtggtg gcatcgggag catcgccctc tccatcctcc
     4201 tcattcacca ccacggccat cactaaagaa gcgatgctgt ccatattggc catgtattgc
     4261 agatgacaag agtggggagc tcttaaagtg ctgccgcaga gggttaaatc gaagctcagc
     4321 ttctcatctt gtaaaaccct agcgtgcttg gcgttgcagt ccacgatcat tctcaccttg
     4381 ttcttcataa ataaaaatct ggcagcttgg gggatgtctg tggcgggata gtgtaaacct
     4441 aaatagggtt ctaaaccggg cttggtcact tcgctcacca cctcgccgtg gtcgtcctcg
     4501 tggaacttgt aggccatgac acgatcgtaa ccagtcagct cgaagacttc ttgcaccatg
     4561 gtgtcgcaca gcctctccat gcttccgctg ggtaaagact ggagtctggt gatggccttg
     4621 gcggccagct tatagctctg taaagctccg gcggctgtca tgggcacttc atagggctta
     4681 acgggttcga agtcgatgat gatggatccg gtcaccctat ggataatggc gtagaagggc
     4741 ttggcggatg tacgacaatg gacgaggata gggttcagta aagacacgtc gccgaagccg
     4801 agagccttct gtaaagcgga agcgctggga gcggtaaata aagatctgat atcggtgccg
     4861 atgcccagaa cggggtgctc tcccacgcta ggcacggcgt gggaagccat tgtgagcagt
     4921 tcggaggcgt tctcggagta ggcgatgacc ttgaaggtct tttcgtctaa agccagtaaa
     4981 catccaaagg gctggatcag cttgcccttc tggatatgat ggaggtaggt tgtggtgacc
     5041 ttgtcgctcc taggaggctg gttctcgacc acgggaccag tcaccctcac gctggtggag
     5101 taatcaaagg aggagccgct ctcctcgaaa tcggcgtgca gtttggcgtc cacggtggtc
     5161 tgagcgatga tacgagcgga gtgacgagat ctacgagatc cctcgctgga ctgtgtggga
     5221 cgagatccgc tcatcttctt ctccatggtg gcggcacgcg tgcgagacga gacgagacag
     5281 cctgagaatg gatgcgagta atgggcgcgc cttcgctcga attaatcaat tctttgccaa
     5341 aatgatgaga cagcacaata accagcacgt tgcccaggag ctgtaggaaa aagaagaagg
     5401 catgaacatg gttagcagag gctctagagc cgccggtcac acgccagaag ccgaaccccg
     5461 ccctgccccg tcccccccga aggcagccgt ccccccgcgg acagccccga ggctggagag
     5521 ggagaagggg acggcggcgc ggcgacgcac gaaggccctc cccgcccatt tccttcctgc
     5581 cggcgccgca ccgcttcgcc ccgcgcccgc tagagggggt gcggcggcgc ctcccagatt
     5641 tcggctccgc acagatttgg gacaaaggaa gtccctgcgc cctctcgcac gattaccata
     5701 aaaggcaatg gctgcggctc gccgcgcctc gacagccgcc ggcgctccgg gggccgccgc
     5761 gcccctcccc cgagccctcc ccggcccgag gcggccccgc cccgcccggc acccccacct
     5821 gccgccaccc cccgcccggc acggcgagcc ccgcgccacg ccccgtacgg agccccgcac
     5881 ccgaagccgg gccgtgctca gcaactcggg gaggggggtg cagggggggt tgcagcccga
     5941 ccgacgcgcc cacaccccct gctcaccccc ccacgcacac accccgcacg cagcctttgt
     6001 tcccctcgca gccccccccg caccgcgggg caccgccccc ggccgcgctc ccctcgcgca
     6061 cactgcggag cgcacaaagc cccgcgccgc gcccgcagcg ctcacagccg ccgggcagcg
     6121 cggagccgca cgcggcgctc cccacgcaca cacacacgca cgcacccccc gagccgctcc
     6181 ccccgcacaa agggccctcc cggagcccct caaggctttc acgcagccac agaaaagaaa
     6241 caagccgtca ttaaaccaag cgctaattac agcccggagg agaagggccg tcccgcccgc
     6301 tcacctgtgg gagtaacgcg gtcagtcaga gccggggcgg gcggcgcgag gcggcggcgg
     6361 agcggggcac ggggcgaagg cagcgcgcag cgactcccgc ccgccgcgcg cttcgctttt
     6421 tatagggccg ccgccgccgc cgcctcgcca taaaaggaaa ctttcggagc gcgccgctct
     6481 gattggctgc cgccgcacct ctccgcctcg ccccgccccg cccccccccc cccccccccc
     6541 ccccccggcc cccgcccccc cccccccccc gcccccatcg ctgcacaaaa taattaaaaa
     6601 ataaataaat acaaaattgg gggtggggag gggggggaga tggggagagt gaagcagaac
     6661 gtggggctca cctcgaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta
     6721 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
     6781 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
     6841 taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga
     6901 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
     6961 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
     7021 attgattact attaataact agtcaataat caatgtcgac ccaggtggca cttttcgggg
     7081 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     7141 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     7201 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     7261 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgg
//
