LOCUS       sequence-465003-        7467 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Red light-inducible Vcre-C; Vcre-278C-PhyA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7467)
  AUTHORS   Tous C, Kinstlinger IS, Rice MEL, Deng J, Wong WW
  TITLE     Multiplexing light-inducible recombinases to control cell fate, 
            Boolean logic, and cell patterning in mammalian cells.
  JOURNAL   Sci Adv. 2025 May 9;11(19):eadt1971. doi: 10.1126/sciadv.adt1971. 
            Epub 2025 May 9.
  PUBMED    40344052
REFERENCE   2  (bases 1 to 7467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-05-9"; volume: "11(19):eadt1971. doi"; pages: " 
            10.1126/sciadv.adt1971. Epub 2025 May 9"
COMMENT     Sequence Label: CT534
FEATURES             Location/Qualifiers
     source          1..7467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     438..457
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(676..780)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        811..1190
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1192..1468
                     /label=chicken beta-actin promoter
     intron          1469..2485
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2409..2431
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2493..2512
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2614..2623
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4712..4721
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4822..4842
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4852..5076
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5316..5335)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5381..5436
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5435..5454)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5797..5813)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5797..5813)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5810..5832)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5821..5837
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5845..5851,5852..5869,5870..5875))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5890..5911
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      5996..6130
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6150..6284
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6187..6206)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6241..6260
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6352..6369)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6523..7111)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6603..6622)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7282..7467,1..606,607..675))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
       61 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
      121 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
      181 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
      241 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
      301 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
      361 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
      421 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
      481 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
      541 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
      601 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
      661 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
      721 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
      781 cacatttccc cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt
      841 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      901 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      961 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     1021 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     1081 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     1141 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga
     1201 gccccacgtt ctgcttcact ctccccatct cccccccctc ccccccccca attttgtatt
     1261 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg ggcgcgcgcc
     1321 ggggggggcg gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc
     1381 caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc
     1441 ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc
     1501 ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca
     1561 ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg
     1621 gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg
     1681 cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg
     1741 ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg
     1801 cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga
     1861 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
     1921 gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc
     1981 ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg
     2041 gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga
     2101 ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg
     2161 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga
     2221 gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg
     2281 cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct
     2341 tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
     2401 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt
     2461 tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca
     2521 tcattttggc aaagaattga ttaattcgag cgaaggcgcg cccattactc gcatccattc
     2581 tcaggctgtc tcgtctcgtc tcgcacgcgt gccgccacca tggagaagaa gatgagcgga
     2641 tctcgtccca cacagtccag cgagggatct cgtagatctc gtcactccgc tcgtatcatc
     2701 gctcagacca ccgtggacgc caaactgcac gccgatttcg aggagagcgg ctcctccttt
     2761 gattactcca ccagcgtgag ggtgactggt cccgtggtcg agaaccagcc tcctaggagc
     2821 gacaaggtca ccacaaccta cctccatcat atccagaagg gcaagctgat ccagcccttt
     2881 ggatgtttac tggctttaga cgaaaagacc ttcaaggtca tcgcctactc cgagaacgcc
     2941 tccgaactgc tcacaatggc ttcccacgcc gtgcctagcg tgggagagca ccccgttctg
     3001 ggcatcggca ccgatatcag atctttattt accgctccca gcgcttccgc tttacagaag
     3061 gctctcggct tcggcgacgt gtctttactg aaccctatcc tcgtccattg tcgtacatcc
     3121 gccaagccct tctacgccat tatccatagg gtgaccggat ccatcatcat cgacttcgaa
     3181 cccgttaagc cctatgaagt gcccatgaca gccgccggag ctttacagag ctataagctg
     3241 gccgccaagg ccatcaccag actccagtct ttacccagcg gaagcatgga gaggctgtgc
     3301 gacaccatgg tgcaagaagt cttcgagctg actggttacg atcgtgtcat ggcctacaag
     3361 ttccacgagg acgaccacgg cgaggtggtg agcgaagtga ccaagcccgg tttagaaccc
     3421 tatttaggtt tacactatcc cgccacagac atcccccaag ctgccagatt tttatttatg
     3481 aagaacaagg tgagaatgat cgtggactgc aacgccaagc acgctagggt tttacaagat
     3541 gagaagctga gcttcgattt aaccctctgc ggcagcactt taagagctcc ccactcttgt
     3601 catctgcaat acatggccaa tatggacagc atcgcttctt tagtgatggc cgtggtggtg
     3661 aatgaggagg atggagaggg cgatgctccc gatgccacca cacagcctca gaagagaaag
     3721 aggctgtggg gtttagtggt ctgccacaac accaccccca gatttgtccc ttttccctta
     3781 cgttatgctt gtgaattttt agcccaagtg tttgccatcc acgtcaacaa ggaggtcgag
     3841 ctggacaacc agatggtgga gaagaacatt ttacgtaccc agacactcct ctgcgacatg
     3901 ctcatgaggg acgctcccct cggcatcgtg agccagtccc ccaatattat ggatttagtc
     3961 aagtgcgacg gcgccgcctt attatacaag gacaagatct ggaagctggg caccacccct
     4021 agcgagttcc atttacaaga aatcgcttct tggctgtgtg agtaccacat ggattccacc
     4081 ggtttaagca ccgattcttt acacgacgct ggctttccta gggctttatc tttaggcgac
     4141 agcgtctgcg gaatggctgc cgttcgtatc agcagcaaag atatgatttt ctggtttcgt
     4201 tcccataccg ccggcgaagt gagatggggc ggcgccaaac acgaccccga tgatagggac
     4261 gatgccagaa ggatgcaccc cagatcctcc ttcaaggctt ttctggaggt ggtgaagacc
     4321 agaagcttac cttggaagga ctacgaaatg gatgccatcc actctttaca gctcatctta
     4381 cgtaacgcct ttaaggacag cgagaccacc gacgtgaaca caaaggtgat ctatagcaag
     4441 ctcaacgatc tcaagattga tggaatccaa tccggagggt ctggctccgg atcaagtggt
     4501 ggcagcggta ccggcgaggt gctgcacaag cccatcacca ccaagacagt ggaaggcgtg
     4561 ttctacagcg cctgggagac actggacctg ggcagacagg gcgtgaagcc tttcacagcc
     4621 cacagcgcca gagtgggagc cgctcaggac ctgctgaaga agggctacaa taccctgcag
     4681 atccagcagt ccggccggtg gtctagcgga gccatggtgg ccagatacgg cagagccatc
     4741 ctggctaggg atggcgctat ggcccacagc agagtgaaaa ccagatccgc ccccatgcag
     4801 tggggcaagg acgagaagga ccccaagaaa aagcggaagg tgtgagaatt cctgtgcctt
     4861 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     4921 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     4981 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     5041 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     5101 ggggctctag ggggtatccc cacgcgccct gtagcgtgta ccgctgggag ttcgtagacg
     5161 gaaacaaacg cagaatccaa gcgcggccgc ccaggataca tagattacca caactccgag
     5221 cccttccacc gctagcgcac tcctcaggtg caggctgcct atcagaaggt ggtggctggt
     5281 gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat
     5341 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc
     5401 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
     5461 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg
     5521 ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct
     5581 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt
     5641 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc
     5701 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga
     5761 tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     5821 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     5881 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     5941 gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg
     6001 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     6061 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     6121 tttggaggcc taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca
     6181 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6241 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgctgc attaatgaat
     6301 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     6361 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     6421 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     6481 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6541 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6601 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     6661 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     6721 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6781 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6841 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6901 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     6961 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7021 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7081 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7141 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7201 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     7261 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     7321 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     7381 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     7441 tccagattta tcagcaataa accagcc
//
