LOCUS       sequence-465008-        6742 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Red light-inducible Vcre-N; Vcre-N277-FHY1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6742)
  AUTHORS   Tous C, Kinstlinger IS, Rice MEL, Deng J, Wong WW
  TITLE     Multiplexing light-inducible recombinases to control cell fate, 
            Boolean logic, and cell patterning in mammalian cells.
  JOURNAL   Sci Adv. 2025 May 9;11(19):eadt1971. doi: 10.1126/sciadv.adt1971. 
            Epub 2025 May 9.
  PUBMED    40344052
REFERENCE   2  (bases 1 to 6742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-05-9"; volume: "11(19):eadt1971. doi"; pages: " 
            10.1126/sciadv.adt1971. Epub 2025 May 9"
COMMENT     Sequence Label: CT533
FEATURES             Location/Qualifiers
     source          1..6742
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        50..154
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(155..223,224..1015)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(373..392)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1186..1774
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1675..1694
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1928..1945
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    2013..2147
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2037..2056)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2091..2110
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      2167..2301
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    2386..2407
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2422..2427,2428..2445,2446..2452)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2460..2476
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2465..2487
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2484..2500
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2484..2500
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2843..2862
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    2861..2916
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     2962..2981
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3221..3445
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3455..3475)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5059..5078)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          5086..6102
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(5140..5162)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(6103..6380)
                     /label=chicken beta-actin promoter
     enhancer        join(6382..6742,1..19)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ctagtcaata atcaatgtcg acccaggtgg cacttttcgg ggaaatgtgc gcggaacccc
       61 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      121 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
      181 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
      241 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
      301 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
      361 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
      421 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
      481 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
      541 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
      601 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
      661 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
      721 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
      781 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
      841 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
      901 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
      961 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     1021 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     1081 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     1141 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1201 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1261 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1321 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1381 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1441 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1501 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1561 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1621 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1681 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     1741 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     1801 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     1861 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     1921 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     1981 ccccgcgcgt tggccgattc attaatgcag cggatccaga catgataaga tacattgatg
     2041 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     2101 atgctattgc tttatttgta accattataa gctgcaataa acaagttagc tttttgcaaa
     2161 agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg
     2221 cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac
     2281 tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcgga tccgctggca
     2341 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     2401 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     2461 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgg
     2521 gctgcaggtc gagggatctc cataagagaa gagggacagc tatgactggg agtagtcagg
     2581 agaggaggaa aaatctggct agtaaaacat gtaaggaaaa ttttagggat gttaaagaaa
     2641 aaaataacac aaaacaaaat ataaaaaaaa tctaacctca agtcaaggct tttctatgga
     2701 ataaggaatg gacagcaggg ggctgtttca tatactgatg acctctttat agccaacctt
     2761 tgttcatggc agccagcata tgggcatatg ttgccaaact ctaaaccaaa tactcattct
     2821 gatgttttaa atgatttgcc ctcccatatg tccttccgag tgagagacac aaaaaattcc
     2881 aacacactat tgcaatgaaa ataaatttcc tttattagcc agaagtcaga tgctcaaggg
     2941 gcttcatgat gtccccataa tttttggcag agggaaaaag atctcagtgg tatttgtgag
     3001 ccagggcatt ggccacacca gccaccacct tctgataggc agcctgcacc tgaggagtgc
     3061 gctagcggtg gaagggctcg gagttgtggt aatctatgta tcctgggcgg ccgcgcttgg
     3121 attctgcgtt tgtttccgtc tacgaactcc cagcggtaca cgctacaggg cgcgtgggga
     3181 taccccctag agccccagct ggttctttcc gcctcagaag ccatagagcc caccgcatcc
     3241 ccagcatgcc tgctattgtc ttcccaatcc tcccccttgc tgtcctgccc caccccaccc
     3301 cccagaatag aatgacacct actcagacaa tgcgatgcaa tttcctcatt ttattaggaa
     3361 aggacagtgg gagtggcacc ttccagggtc aaggaaggca cgggggaggg gcaaacaaca
     3421 gatggctggc aactagaagg cacaggaatt ctcacacctt ccgctttttc ttgggcagca
     3481 ttaaagtgct aaagtactgc tcgaattctt ggtcgatggt gggcttcttg gtttcgtgta
     3541 aagtgctatc atcttggttc acggtccaac gaccagagga cagcacgaac ttggcagcgt
     3601 tgctgctaca gcccttggcg tcctcctcct cctcttcttg ctcgtcgagg tacagaatgt
     3661 tctcgatcac cttctcggag aagtcctcca gattctgggt gccgtagata tagtctaaat
     3721 gggactcgaa gctgatcatc ttgtcctctc cgcagctgcc gctggattct tccttcatct
     3781 cgatgtcgtt gttgcactcc atggaggtgg tggagttcac gtagctggac atgctgtact
     3841 cggtatcgat ctcgctgttg ccgttggtgt tctcgctaca agccacgttg gcaaagcatg
     3901 tgtgtttgga taaaggtaat aagctcagct cgtcgctctg gtcggtctgg aacttcctct
     3961 tcttggacac ctccacctcc tccatcttca gcacgttctt gctgctgctg ataatcatgt
     4021 ggtggaagct gttgatctcg ctgggcttct cgttgttgtt gtcgacctcc acctcggggg
     4081 taccgctgcc accacttgat ccggagccag accctccgga ggtctgcttg tccttcttgg
     4141 gcttgataca ggtgttgtgc ttggacacgc ccacgaacag gaagccgtcg ctgctcatat
     4201 ccagcttgcc ggcctcggtg tagtccatca gcagggacac cacatcctgg gacaggatgc
     4261 aggtatcggg ctcgccggag tggttggtct tggtgatagg gatggtcagg atggcggttc
     4321 cgtcgccggc cagttccata tcggacaccc ggatgttggc cagctcgctg gctctcagca
     4381 tgctctcgta ggccacagcc agcagagcca ggttccgccg cagcagcagc ttgtcgcttc
     4441 tgtaccacag gctggtcagc ttcagcagat gctgctcgtt gaaagggctg gcctgcttca
     4501 cggcctcgcc ttcccgcact ttcttccggg caatggcctt cagccgatct tccacgtaga
     4561 tgtccaggca gggatcaggg catccggcca ctctgtgcac ccggctgatg gcccatctgt
     4621 acacgctcag ggtgttccgg tgcagttcct cggcccgctc gatgaagaag gcttccacgg
     4681 tatcgggggt ggcaggcagg ctgtgtctgt ggttggccag gcaccaatcc acgaagcagg
     4741 tccaggcgct ttccagccgc cgcagggtgt tggggctgta tctctcttcc cgcttcttga
     4801 actcgttcag cagatgctcg aaggcggcct tgaactgctc gttctcggcc acgttgatgc
     4861 cctttttctg ggcggcctga atggcggttt tcacatcgtc gggccgcacg ccagaaaagt
     4921 cgcccagcag gctcagctgg ttctcgatca tggtggcggc acgcgtgcga gacgagacga
     4981 gacagcctga gaatggatgc gagtaatggg cgcgccttcg ctcgaattaa tcaattcttt
     5041 gccaaaatga tgagacagca caataaccag cacgttgccc aggagctgta ggaaaaagaa
     5101 gaaggcatga acatggttag cagaggctct agagccgccg gtcacacgcc agaagccgaa
     5161 ccccgccctg ccccgtcccc cccgaaggca gccgtccccc cgcggacagc cccgaggctg
     5221 gagagggaga aggggacggc ggcgcggcga cgcacgaagg ccctccccgc ccatttcctt
     5281 cctgccggcg ccgcaccgct tcgccccgcg cccgctagag ggggtgcggc ggcgcctccc
     5341 agatttcggc tccgcacaga tttgggacaa aggaagtccc tgcgccctct cgcacgatta
     5401 ccataaaagg caatggctgc ggctcgccgc gcctcgacag ccgccggcgc tccgggggcc
     5461 gccgcgcccc tcccccgagc cctccccggc ccgaggcggc cccgccccgc ccggcacccc
     5521 cacctgccgc caccccccgc ccggcacggc gagccccgcg ccacgccccg tacggagccc
     5581 cgcacccgaa gccgggccgt gctcagcaac tcggggaggg gggtgcaggg ggggttgcag
     5641 cccgaccgac gcgcccacac cccctgctca cccccccacg cacacacccc gcacgcagcc
     5701 tttgttcccc tcgcagcccc ccccgcaccg cggggcaccg cccccggccg cgctcccctc
     5761 gcgcacactg cggagcgcac aaagccccgc gccgcgcccg cagcgctcac agccgccggg
     5821 cagcgcggag ccgcacgcgg cgctccccac gcacacacac acgcacgcac cccccgagcc
     5881 gctccccccg cacaaagggc cctcccggag cccctcaagg ctttcacgca gccacagaaa
     5941 agaaacaagc cgtcattaaa ccaagcgcta attacagccc ggaggagaag ggccgtcccg
     6001 cccgctcacc tgtgggagta acgcggtcag tcagagccgg ggcgggcggc gcgaggcggc
     6061 ggcggagcgg ggcacggggc gaaggcagcg cgcagcgact cccgcccgcc gcgcgcttcg
     6121 ctttttatag ggccgccgcc gccgccgcct cgccataaaa ggaaactttc ggagcgcgcc
     6181 gctctgattg gctgccgccg cacctctccg cctcgccccg ccccgcccct cgccccgccc
     6241 cgccccgcct ggcgcgcgcc cccccccccc ccccgccccc atcgctgcac aaaataatta
     6301 aaaaataaat aaatacaaaa ttgggggtgg ggaggggggg gagatgggga gagtgaagca
     6361 gaacgtgggg ctcacctcga ccatggtaat agcgatgact aatacgtaga tgtactgcca
     6421 agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc
     6481 attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc
     6541 agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat
     6601 gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg
     6661 gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc
     6721 cgtaattgat tactattaat aa
//
