LOCUS       sequence-46501-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, 
            Waterman ML
  TITLE     A uniform human Wnt expression library reveals a shared secretory 
            pathway and unique signaling activities.
  JOURNAL   Differentiation. 2012 Sep;84(2):203-13. doi: 
            10.1016/j.diff.2012.06.004. Epub 2012 Jul 9.
  PUBMED    22784633
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.diff.2012.06.004"; journalName: "Differentiation"; date: 
            "2012-09"; volume: "84"; issue: "2"; pages: "203-13"
COMMENT     Sequence Label: pcDNA-Wnt9B-V5
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    102..126
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag ttaagctatc aacaagtttg tacaaaaaag
      121 caggctccgc ggccgccccc ttcaccatgc gccccccgcc cgcgctggcc ctggccgggc
      181 tctgcctgct ggcgctgccc gccgccgccg cctcctactt cggcctgacc gggcgggaag
      241 tcctgacgcc cttcccagga ttgggcactg cggcagcccc ggcacagggc ggggcccacc
      301 tgaagcagtg tgacctgctg aagctgtccc ggcggcagaa gcagctctgc cggagggagc
      361 ccggcctggc tgagaccctg agggatgctg cgcacctcgg cctgcttgag tgccagtttc
      421 agttccggca tgagcgctgg aactgtagcc tggagggcag gacgggcctg ctcaagagag
      481 gcttcaaaga gacagctttc ctgtacgcgg tgtcctctgc cgccctcacc cacaccctgg
      541 cccgggcctg cagcgctggg cgcatggagc gctgcacctg tgatgactct ccggggctgg
      601 agagccggca ggcctggcag tggggcgtgt gcggtgacaa cctcaagtac agcaccaagt
      661 ttctgagcaa cttcctgggg tccaagagag gaaacaagga cctgcgggca cgggcagacg
      721 cccacaatac ccacgtgggc atcaaggctg tgaagagtgg cctcaggacc acgtgtaagt
      781 gccatggcgt atcaggctcc tgtgccgtgc gcacctgctg gaagcagctc tccccgttcc
      841 gtgagacggg ccaggtgctg aaactgcgct atgactcggc tgtcaaggtg tcc
//
