LOCUS       sequence-465010-        7908 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Red light-inducible Cre-N; Cre-N251-PhyA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7908)
  AUTHORS   Tous C, Kinstlinger IS, Rice MEL, Deng J, Wong WW
  TITLE     Multiplexing light-inducible recombinases to control cell fate, 
            Boolean logic, and cell patterning in mammalian cells.
  JOURNAL   Sci Adv. 2025 May 9;11(19):eadt1971. doi: 10.1126/sciadv.adt1971. 
            Epub 2025 May 9.
  PUBMED    40344052
REFERENCE   2  (bases 1 to 7908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-05-9"; volume: "11(19):eadt1971. doi"; pages: " 
            10.1126/sciadv.adt1971. Epub 2025 May 9"
COMMENT     Sequence Label: CT617
FEATURES             Location/Qualifiers
     source          1..7908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(101..120)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      914..1502
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1403..1422
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1656..1673
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    1741..1875
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1765..1784)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1819..1838
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      1895..2029
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    2114..2135
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2150..2155,2156..2173,2174..2180)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2188..2204
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2193..2215
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2212..2228
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2212..2228
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2571..2590
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    2589..2644
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     2690..2709
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2949..3173
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3183..3203)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5954..5973)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          5981..6997
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(6035..6057)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(6998..7274)
                     /label=chicken beta-actin promoter
     enhancer        7276..7655
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7686..7790
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7791..7859,7860..7908,1..743)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
       61 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
      121 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
      181 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
      241 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
      301 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
      361 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
      421 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
      481 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
      541 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
      601 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
      661 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
      721 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
      781 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
      841 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
      901 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      961 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     1021 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     1081 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     1141 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     1201 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     1261 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     1321 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     1381 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     1441 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     1501 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1561 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1621 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1681 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagcg
     1741 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     1801 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     1861 tgcaataaac aagttagctt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac
     1921 ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt
     1981 cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggac tatggttgct
     2041 gactaattga gatgcggatc cgctggcacg acaggtttcc cgactggaaa gcgggcagtg
     2101 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     2161 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     2221 gctatgacca tgattacgcc aagcttgggc tgcaggtcga gggatctcca taagagaaga
     2281 gggacagcta tgactgggag tagtcaggag aggaggaaaa atctggctag taaaacatgt
     2341 aaggaaaatt ttagggatgt taaagaaaaa aataacacaa aacaaaatat aaaaaaaatc
     2401 taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg ctgtttcata
     2461 tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg ggcatatgtt
     2521 gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct cccatatgtc
     2581 cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat aaatttcctt
     2641 tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt tttggcagag
     2701 ggaaaaagat ctcagtggta tttgtgagcc agggcattgg ccacaccagc caccaccttc
     2761 tgataggcag cctgcacctg aggagtgcgc tagcggtgga agggctcgga gttgtggtaa
     2821 tctatgtatc ctgggcggcc gcgcttggat tctgcgtttg tttccgtcta cgaactccca
     2881 gcggtacacg ctacagggcg cgtggggata ccccctagag ccccagctgg ttctttccgc
     2941 ctcagaagcc atagagccca ccgcatcccc agcatgcctg ctattgtctt cccaatcctc
     3001 ccccttgctg tcctgcccca ccccaccccc cagaatagaa tgacacctac tcagacaatg
     3061 cgatgcaatt tcctcatttt attaggaaag gacagtggga gtggcacctt ccagggtcaa
     3121 ggaaggcacg ggggaggggc aaacaacaga tggctggcaa ctagaaggca caggaattct
     3181 cacaccttcc gctttttctt gggttggatt ccatcaatct tgagatcgtt gagcttgcta
     3241 tagatcacct ttgtgttcac gtcggtggtc tcgctgtcct taaaggcgtt acgtaagatg
     3301 agctgtaaag agtggatggc atccatttcg tagtccttcc aaggtaagct tctggtcttc
     3361 accacctcca gaaaagcctt gaaggaggat ctggggtgca tccttctggc atcgtcccta
     3421 tcatcggggt cgtgtttggc gccgccccat ctcacttcgc cggcggtatg ggaacgaaac
     3481 cagaaaatca tatctttgct gctgatacga acggcagcca ttccgcagac gctgtcgcct
     3541 aaagataaag ccctaggaaa gccagcgtcg tgtaaagaat cggtgcttaa accggtggaa
     3601 tccatgtggt actcacacag ccaagaagcg atttcttgta aatggaactc gctaggggtg
     3661 gtgcccagct tccagatctt gtccttgtat aataaggcgg cgccgtcgca cttgactaaa
     3721 tccataatat tgggggactg gctcacgatg ccgaggggag cgtccctcat gagcatgtcg
     3781 cagaggagtg tctgggtacg taaaatgttc ttctccacca tctggttgtc cagctcgacc
     3841 tccttgttga cgtggatggc aaacacttgg gctaaaaatt cacaagcata acgtaaggga
     3901 aaagggacaa atctgggggt ggtgttgtgg cagaccacta aaccccacag cctctttctc
     3961 ttctgaggct gtgtggtggc atcgggagca tcgccctctc catcctcctc attcaccacc
     4021 acggccatca ctaaagaagc gatgctgtcc atattggcca tgtattgcag atgacaagag
     4081 tggggagctc ttaaagtgct gccgcagagg gttaaatcga agctcagctt ctcatcttgt
     4141 aaaaccctag cgtgcttggc gttgcagtcc acgatcattc tcaccttgtt cttcataaat
     4201 aaaaatctgg cagcttgggg gatgtctgtg gcgggatagt gtaaacctaa atagggttct
     4261 aaaccgggct tggtcacttc gctcaccacc tcgccgtggt cgtcctcgtg gaacttgtag
     4321 gccatgacac gatcgtaacc agtcagctcg aagacttctt gcaccatggt gtcgcacagc
     4381 ctctccatgc ttccgctggg taaagactgg agtctggtga tggccttggc ggccagctta
     4441 tagctctgta aagctccggc ggctgtcatg ggcacttcat agggcttaac gggttcgaag
     4501 tcgatgatga tggatccggt caccctatgg ataatggcgt agaagggctt ggcggatgta
     4561 cgacaatgga cgaggatagg gttcagtaaa gacacgtcgc cgaagccgag agccttctgt
     4621 aaagcggaag cgctgggagc ggtaaataaa gatctgatat cggtgccgat gcccagaacg
     4681 gggtgctctc ccacgctagg cacggcgtgg gaagccattg tgagcagttc ggaggcgttc
     4741 tcggagtagg cgatgacctt gaaggtcttt tcgtctaaag ccagtaaaca tccaaagggc
     4801 tggatcagct tgcccttctg gatatgatgg aggtaggttg tggtgacctt gtcgctccta
     4861 ggaggctggt tctcgaccac gggaccagtc accctcacgc tggtggagta atcaaaggag
     4921 gagccgctct cctcgaaatc ggcgtgcagt ttggcgtcca cggtggtctg agcgatgata
     4981 cgagcggagt gacgagatct acgagatccc tcgctggact gtgtgggacg agatccgctc
     5041 atcttcttct cggtaccgct gccaccactt gatccggagc cagaccctcc ggaagaaggg
     5101 gcagccacac cattctttct gacccggcag aacaggtagt tgttggggtc atcagccaca
     5161 ccagacacag agatccatct ctccaccagc ttggtaaccc ccagggacag ggccttctcc
     5221 acaccagctg tggacaccag ggtcttggtc ctgccaatgt ggatcagcat tctcccacca
     5281 tcggtgcggg agatgtcctt cactctgatt ctggcaattt cggcaatgcg cagcagggtg
     5341 ttgtaggcaa tgcccaggaa ggccaggttc ctgatgtcct ggcatctgtc agagttctcc
     5401 atcagggatc tgacttggtc aaagtcagtg cgttcaaagg ccagggcctg cttggctctc
     5461 tccccagcat ccacattctc ctttctgatt ctcctcatca ccagggacac agcattggag
     5521 tcagaagggc gaggcaggcc agatctcctg tgcagcatgt tgagctggcc caggtgctgt
     5581 tggatggtct tcacagccag gcctctggct tgcaggtaca ggaggtagtc cctcacatcc
     5641 tcaggttcag cagggaacca tttcctgttg ttcagcttgc accaggcagc ccaggatctg
     5701 cacacagaca ggagcatctt ccaggtgtgt tcagagaagg cctgcctgtc cctgaacatg
     5761 tccatcaggt tcttcctgac ttcatcagag gtggcatcca cagggagggc aggcaggttt
     5821 tggtgcacag tcagcaggtt ggacatggtg gcggcacgcg tgcgagacga gacgagacag
     5881 cctgagaatg gatgcgagta atgggcgcgc cttcgctcga attaatcaat tctttgccaa
     5941 aatgatgaga cagcacaata accagcacgt tgcccaggag ctgtaggaaa aagaagaagg
     6001 catgaacatg gttagcagag gctctagagc cgccggtcac acgccagaag ccgaaccccg
     6061 ccctgccccg tcccccccga aggcagccgt ccccccgcgg acagccccga ggctggagag
     6121 ggagaagggg acggcggcgc ggcgacgcac gaaggccctc cccgcccatt tccttcctgc
     6181 cggcgccgca ccgcttcgcc ccgcgcccgc tagagggggt gcggcggcgc ctcccagatt
     6241 tcggctccgc acagatttgg gacaaaggaa gtccctgcgc cctctcgcac gattaccata
     6301 aaaggcaatg gctgcggctc gccgcgcctc gacagccgcc ggcgctccgg gggccgccgc
     6361 gcccctcccc cgagccctcc ccggcccgag gcggccccgc cccgcccggc acccccacct
     6421 gccgccaccc cccgcccggc acggcgagcc ccgcgccacg ccccgtacgg agccccgcac
     6481 ccgaagccgg gccgtgctca gcaactcggg gaggggggtg cagggggggt tgcagcccga
     6541 ccgacgcgcc cacaccccct gctcaccccc ccacgcacac accccgcacg cagcctttgt
     6601 tcccctcgca gccccccccg caccgcgggg caccgccccc ggccgcgctc ccctcgcgca
     6661 cactgcggag cgcacaaagc cccgcgccgc gcccgcagcg ctcacagccg ccgggcagcg
     6721 cggagccgca cgcggcgctc cccacgcaca cacacacgca cgcacccccc gagccgctcc
     6781 ccccgcacaa agggccctcc cggagcccct caaggctttc acgcagccac agaaaagaaa
     6841 caagccgtca ttaaaccaag cgctaattac agcccggagg agaagggccg tcccgcccgc
     6901 tcacctgtgg gagtaacgcg gtcagtcaga gccggggcgg gcggcgcgag gcggcggcgg
     6961 agcggggcac ggggcgaagg cagcgcgcag cgactcccgc ccgccgcgcg cttcgctttt
     7021 tatagggccg ccgccgccgc cgcctcgcca taaaaggaaa ctttcggagc gcgccgctct
     7081 gattggctgc cgccgcacct ctccgcctcc ccccgccccc cccctcgccc ccccccgccc
     7141 cgcccggcgc gcgccccccc ccccccccgc ccccatcgct gcacaaaata attaaaaaat
     7201 aaataaatac aaaattgggg gtggggaggg gggggagatg gggagagtga agcagaacgt
     7261 ggggctcacc tcgaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg
     7321 aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac
     7381 gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta
     7441 ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac
     7501 atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt
     7561 taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat
     7621 tgattactat taataactag tcaataatca atgtcgaccc aggtggcact tttcggggaa
     7681 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     7741 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     7801 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     7861 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtg
//
