LOCUS       sequence-465011-        6135 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Red light-inducible Cre-C; Cre-271C-FHY1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Tous C, Kinstlinger IS, Rice MEL, Deng J, Wong WW
  TITLE     Multiplexing light-inducible recombinases to control cell fate, 
            Boolean logic, and cell patterning in mammalian cells.
  JOURNAL   Sci Adv. 2025 May 9;11(19):eadt1971. doi: 10.1126/sciadv.adt1971. 
            Epub 2025 May 9.
  PUBMED    40344052
REFERENCE   2  (bases 1 to 6135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-05-9"; volume: "11(19):eadt1971. doi"; pages: " 
            10.1126/sciadv.adt1971. Epub 2025 May 9"
COMMENT     Sequence Label: CT614
FEATURES             Location/Qualifiers
     source          1..6135
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(106..125)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      919..1507
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1408..1427
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1661..1678
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    1746..1880
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1770..1789)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1824..1843
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      1900..2034
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    2119..2140
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2155..2160,2161..2178,2179..2185)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2193..2209
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2198..2220
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2217..2233
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2217..2233
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2576..2595
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    2594..2649
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     2695..2714
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2954..3178
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3188..3208)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4183..4202)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          4210..5226
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(4264..4286)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(5227..5506)
                     /label=chicken beta-actin promoter
     enhancer        5508..5887
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5918..6022
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6023..6091,6092..6135,1..748)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
       61 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
      121 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
      181 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
      241 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
      301 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
      361 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
      421 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
      481 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
      541 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
      601 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
      661 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
      721 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
      781 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
      841 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
      901 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
      961 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     1021 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     1081 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     1141 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     1201 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     1261 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     1321 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     1381 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     1441 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     1501 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     1561 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     1621 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     1681 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     1741 cagcggatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     1801 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     1861 taagctgcaa taaacaagtt agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     1921 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     1981 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggactatgg
     2041 ttgctgacta attgagatgc ggatccgctg gcacgacagg tttcccgact ggaaagcggg
     2101 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     2161 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     2221 aaacagctat gaccatgatt acgccaagct tgggctgcag gtcgagggat ctccataaga
     2281 gaagagggac agctatgact gggagtagtc aggagaggag gaaaaatctg gctagtaaaa
     2341 catgtaagga aaattttagg gatgttaaag aaaaaaataa cacaaaacaa aatataaaaa
     2401 aaatctaacc tcaagtcaag gcttttctat ggaataagga atggacagca gggggctgtt
     2461 tcatatactg atgacctctt tatagccaac ctttgttcat ggcagccagc atatgggcat
     2521 atgttgccaa actctaaacc aaatactcat tctgatgttt taaatgattt gccctcccat
     2581 atgtccttcc gagtgagaga cacaaaaaat tccaacacac tattgcaatg aaaataaatt
     2641 tcctttatta gccagaagtc agatgctcaa ggggcttcat gatgtcccca taatttttgg
     2701 cagagggaaa aagatctcag tggtatttgt gagccagggc attggccaca ccagccacca
     2761 ccttctgata ggcagcctgc acctgaggag tgcgctagcg gtggaagggc tcggagttgt
     2821 ggtaatctat gtatcctggg cggccgcgct tggattctgc gtttgtttcc gtctacgaac
     2881 tcccagcggt acacgctaca gggcgcgtgg ggataccccc tagagcccca gctggttctt
     2941 tccgcctcag aagccataga gcccaccgca tccccagcat gcctgctatt gtcttcccaa
     3001 tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca cctactcaga
     3061 caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc accttccagg
     3121 gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga aggcacagga
     3181 attctcacac cttccgcttt ttcttggggt ccccatcctc gagcagcctc accatggccc
     3241 cagtctcaga gtccaggttt ctgatgtagt tcatcacaat gttcacattg gtccagccac
     3301 cagcctgcat gatttcaggg atggacacac cagccctggc catgtccctg gcagcaccca
     3361 ctctggcaga gtggccagac caggccaggt atctctgccc agagtcatcc ttggcaccat
     3421 agatcagggt accgctgcca ccacttgatc cggagccaga ccctccggac agcattaaag
     3481 tgctaaagta ctgctcgaat tcttggtcga tggtgggctt cttggtttcg tgtaaagtgc
     3541 tatcatcttg gttcacggtc caacgaccag aggacagcac gaacttggca gcgttgctgc
     3601 tacagccctt ggcgtcctcc tcctcctctt cttgctcgtc gaggtacaga atgttctcga
     3661 tcaccttctc ggagaagtcc tccagattct gggtgccgta gatatagtct aaatgggact
     3721 cgaagctgat catcttgtcc tctccgcagc tgccgctgga ttcttccttc atctcgatgt
     3781 cgttgttgca ctccatggag gtggtggagt tcacgtagct ggacatgctg tactcggtat
     3841 cgatctcgct gttgccgttg gtgttctcgc tacaagccac gttggcaaag catgtgtgtt
     3901 tggataaagg taataagctc agctcgtcgc tctggtcggt ctggaacttc ctcttcttgg
     3961 acacctccac ctcctccatc ttcagcacgt tcttgctgct gctgataatc atgtggtgga
     4021 agctgttgat ctcgctgggc ttctcgttgt tgttgtcgac ctccacctcg ggcatggtgg
     4081 cggcacgcgt gcgagacgag acgagacagc ctgagaatgg atgcgagtaa tgggcgcgcc
     4141 ttcgctcgaa ttaatcaatt ctttgccaaa atgatgagac agcacaataa ccagcacgtt
     4201 gcccaggagc tgtaggaaaa agaagaaggc atgaacatgg ttagcagagg ctctagagcc
     4261 gccggtcaca cgccagaagc cgaaccccgc cctgccccgt cccccccgaa ggcagccgtc
     4321 cccccgcgga cagccccgag gctggagagg gagaagggga cggcggcgcg gcgacgcacg
     4381 aaggccctcc ccgcccattt ccttcctgcc ggcgccgcac cgcttcgccc cgcgcccgct
     4441 agagggggtg cggcggcgcc tcccagattt cggctccgca cagatttggg acaaaggaag
     4501 tccctgcgcc ctctcgcacg attaccataa aaggcaatgg ctgcggctcg ccgcgcctcg
     4561 acagccgccg gcgctccggg ggccgccgcg cccctccccc gagccctccc cggcccgagg
     4621 cggccccgcc ccgcccggca cccccacctg ccgccacccc ccgcccggca cggcgagccc
     4681 cgcgccacgc cccgtacgga gccccgcacc cgaagccggg ccgtgctcag caactcgggg
     4741 aggggggtgc agggggggtt gcagcccgac cgacgcgccc acaccccctg ctcacccccc
     4801 cacgcacaca ccccgcacgc agcctttgtt cccctcgcag ccccccccgc accgcggggc
     4861 accgcccccg gccgcgctcc cctcgcgcac actgcggagc gcacaaagcc ccgcgccgcg
     4921 cccgcagcgc tcacagccgc cgggcagcgc ggagccgcac gcggcgctcc ccacgcacac
     4981 acacacgcac gcaccccccg agccgctccc cccgcacaaa gggccctccc ggagcccctc
     5041 aaggctttca cgcagccaca gaaaagaaac aagccgtcat taaaccaagc gctaattaca
     5101 gcccggagga gaagggccgt cccgcccgct cacctgtggg agtaacgcgg tcagtcagag
     5161 ccggggcggg cggcgcgagg cggcggcgga gcggggcacg gggcgaaggc agcgcgcagc
     5221 gactcccgcc cgccgcgcgc ttcgcttttt atagggccgc cgccgccgcc gcctcgccat
     5281 aaaaggaaac tttcggagcg cgccgctctg attggctgcc gccgcacctc tccgcctcgc
     5341 cccgcccccc cccccccccc ccgccccccc ccgcccggcg cgcgcccccc cccccccccc
     5401 gcccccatcg ctgcacaaaa taattaaaaa ataaataaat acaaaattgg gggtggggag
     5461 gggggggaga tggggagagt gaagcagaac gtggggctca cctcgaccat ggtaatagcg
     5521 atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca
     5581 taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga
     5641 tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa
     5701 tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg
     5761 gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc
     5821 atatatgggc tatgaactaa tgaccccgta attgattact attaataact agtcaataat
     5881 caatgtcgac ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     5941 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     6001 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     6061 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     6121 tgctgaagat cagtt
//
