LOCUS       sequence-465044-        6559 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of Isl1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6559)
  AUTHORS   Wang NB, Lende-Dorn BA, Beitz AM, Han P, Adewumi HO, O'Shea TM, 
            Galloway KE
  TITLE     Proliferation history and transcription factor levels drive direct 
            conversion to motor neurons.
  JOURNAL   Cell Syst. 2025 Apr 16;16(4):101205. doi: 
            10.1016/j.cels.2025.101205. Epub 2025 Mar 13.
  PUBMED    40086434
REFERENCE   2  (bases 1 to 6559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Syst."; date: "2025-04-16"; volume: "16(4):101205. doi"; pages: " 
            10.1016/j.cels.2025.101205. Epub 2025 Mar 13"
COMMENT     Sequence Label: pMXs-Isl1
FEATURES             Location/Qualifiers
     source          1..6559
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    574..931
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             990..1406
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1336..1358
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1374..1390
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1416..1790
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1728..1744
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    1833..1857
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2831..2840
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3151..3175)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3192..3780
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3245..3265)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3663..3674)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3960..4429
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4559..4576)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4730..5318)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4810..4829)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5482..6273,6274..6342))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6105..6124
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6343..6447)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             join(6477..6559,1..511)
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 tagaaaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg ccaaacagga
       61 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga acagctgaat
      121 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag
      181 atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag atgtttccag
      241 ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt
      301 ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca
      361 ctcggcgcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc
      421 ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg
      481 attgactacc cgtcagcggg ggtctttcat ttgggggctc gtccgggatc gggagacccc
      541 tgcccaggga ccaccgaccc accaccggga ggtaagctgg ccagcaactt atctgtgtct
      601 gtccgattgt ctagtgtcta tgactgattt tatgcgcctg cgtcggtact agttagctaa
      661 ctagctctgt atctggcgga cccgtggtgg aactgacgag ttcggaacac ccggccgcaa
      721 ccctgggaga cgtcccaggg acttcggggg ccgtttttgt ggcccgacct gagtccaaaa
      781 atcccgatcg ttttggactc tttggtgcac ccccctaata ggagggatat gtggttctgg
      841 taggagacga gaacctaaaa cagttcccgc ctccgtctga atttttgctt tcggtttggg
      901 accgaagccg cgccgcgcgt cttgtctgct gcagcatcgt tctgtgttgt ctctgtctga
      961 ctgtgtttct gtatttgtct gaaaattagg gccagactgt taccactccc ttaagtttga
     1021 ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga
     1081 agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc ggatggccgc
     1141 gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc ttttcacctg
     1201 gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc ttggcttttg
     1261 acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct cttcctccat
     1321 ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc
     1381 cagccctcac tccttctcta ggcgccccca tatggccata tgagatctta tatggggcac
     1441 ccccgcccct tgtaaacttc cctgaccctg acatgacaag agttactaac agcccctctc
     1501 tccaagctca cttacaggct ctctacttag tccagcacga agtctggaga cctctggcgg
     1561 cagcctacca agaacaactg gaccgaccgg tggtacctca cccttaccga gtcggcgaca
     1621 cagtgtgggt ccgccgacac cagactaaga acctagaacc tcgctggaaa ggaccttaca
     1681 cagtcctgct gaccaccccc accgccctca aagtagacgg catcgcagct tggatacacg
     1741 ccgcccacgt gaaggctgcc gaccccgggg gtggaccatc ctctagactg ccggatctag
     1801 ctagttaatc gacggtatcg ataagctatc aaacaagttt gtacaaaaaa gcaggcttgg
     1861 cttaaatcgg actcccagat ctgcgagggc gcggcgcagc cgggcagctg tttcccccag
     1921 ttttggcaac cccgggggcc actatttgcc acctagccac agcaccagca tcctctctgt
     1981 gggctattca ccaattgtcc aaccaccatt tcactgtgga cgttactccc tcttacagat
     2041 atgggagaca tgggcgatcc accaaaaaaa aaacgtctga tttccctgtg tgttggttgc
     2101 ggcaatcaaa ttcacgacca gtatattctg agggtttctc cggatttgga gtggcatgca
     2161 gcatgtttga aatgtgcgga gtgtaatcag tatttggacg aaagctgtac gtgctttgtt
     2221 agggatggga aaacctactg taaaagagat tatatcaggt tgtacgggat caaatgcgcc
     2281 aagtgcagca taggcttcag caagaacgac ttcgtgatgc gcgcccgctc taaggtgtac
     2341 cacatcgagt gtttccgctg tgtagcctgc agccgacagc tcatcccggg agacgaattc
     2401 gccctgcggg aggatgggct tttctgccgt gcagaccacg atgtggtgga gagagccagc
     2461 ctgggagctg gagaccctct cagtcccttg catccagcgc ggcctctgca aatggcagcc
     2521 gaacccatct cggctaggca gccagctctg cggccgcacg tccacaagca gccggagaag
     2581 accacccgag tgcggactgt gctcaacgag aagcagctgc acaccttgcg gacctgctat
     2641 gccgccaacc ctcggccaga tgcgctcatg aaggagcaac tagtggagat gacgggcctc
     2701 agtcccagag tcatccgagt gtggtttcaa aacaagcggt gcaaggacaa gaaacgcagc
     2761 atcatgatga agcagctcca gcagcagcaa cccaacgaca aaactaatat ccaggggatg
     2821 acaggaactc ccatggtggc tgctagtccg gagagacatg atggtggttt acaggctaac
     2881 ccagtagagg tgcaaagtta ccagccgccc tggaaagtac tgagtgactt cgccttgcaa
     2941 agcgacatag atcagcctgc ttttcagcaa ctggtcaatt tttcagaagg aggaccaggc
     3001 tctaattcta ctggcagtga agtagcatcg atgtcctcgc agctcccaga tacacccaac
     3061 agcatggtag ccagtcctat tgaggcatga ggaacattca ttcagatgtt ttgttttgtt
     3121 ttgttttgtt tttttcccct gttggagaat acccagcttt cttgtacaaa gtggttcgat
     3181 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     3241 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     3301 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     3361 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     3421 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     3481 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     3541 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     3601 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     3661 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     3721 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     3781 atcgataccg tcgacgataa aataaaagat tttatttagt ctccagaaaa aggggggaat
     3841 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg
     3901 gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc
     3961 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
     4021 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc
     4081 cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga
     4141 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga
     4201 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata
     4261 aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt
     4321 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt
     4381 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcac atgcagcatg
     4441 tatcaaaatt aatttggttt tttttcttaa gtatttacat taaatggcca tagttgcatt
     4501 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     4561 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     4621 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     4681 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     4741 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     4801 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     4861 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     4921 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     4981 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     5041 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     5101 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     5161 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     5221 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     5281 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     5341 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     5401 caaaaaggat cttcacctag atccttttgc ggccggccgc aaatcaatct aaagtatata
     5461 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     5521 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     5581 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     5641 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     5701 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     5761 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     5821 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     5881 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     5941 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     6001 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     6061 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     6121 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     6181 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     6241 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     6301 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     6361 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     6421 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgcataatg
     6481 aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg
     6541 aaaaatacat aactgagaa
//
