LOCUS       sequence-465046-       10196 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of human Delta-37-PPM1H with C-terminal 
            TMEM115-HA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10196)
  AUTHORS   Wondwossen M Yeshaw , Ayan Adhikari, Claire Y Chiang, Herschel S 
            Dhekne, Paulina S Wawro, Suzanne R Pfeffer
  TITLE     Localization of PPM1H phosphatase tunes Parkinson's disease-linked 
            LRRK2 kinase-mediated Rab GTPase phosphorylation and ciliogenesis
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Oct 31;120(44)
REFERENCE   2  (bases 1 to 10196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2023 Oct 31;120(44)"
COMMENT     Sequence Label: Lenti-TO-Delta-37 PPM1H-TMEM115-HA
FEATURES             Location/Qualifiers
     source          1..10196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             300..356
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             357..755
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    762..1350
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(815..835)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1233..1244)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1424..1657
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1729..1850
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1766..1785)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1820..1839
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1890..2025
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2058..2074)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2058..2074)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2071..2093)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2082..2098
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2106..2112,2113..2130,2131..2136))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2151..2172
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2289..2306)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2460..3048)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(3219..4010,4011..4079))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3842..3861
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4080..4184)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4252..4270
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4308..4330)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4430..4449
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4643..4665
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4657..4674
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4658..4674
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4689..4915
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4916..5096
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5143..5268
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5761..5994
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6179..6223
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6490..6606
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6639..6657
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6675..6693
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6711..6729
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6747..6765
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6783..6801
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6819..6837
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6855..6873
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     6918..6942
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             9155..9181
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        9223..9434
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             9447..9715
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             join(9743..10196,1..290)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 aggtcgccaa agaggaaaga gagacaccca ccaccgacag catgcccccc ctgctgaagc
       61 aggccatcga gctgttcgac agacagggcg ccgagcccgc cttcctgttc ggcctggaac
      121 tgatcatctg cggcctcgag aagcagctga agtgcgagag cggcggaccc accgacgccc
      181 tggacgactt cgacctggac atgctgcccg ccgatgccct ggatgatttt gatctggata
      241 tgctccctgc tgacgctctc gatgacttcg atctcgatat gctgccaggc ggaagcggag
      301 ctactaactt cagcctgctg aagcaggctg gagacgtgga ggagaaccct ggacctatgg
      361 ccaagccttt gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca
      421 gcatccccat ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca
      481 tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc
      541 tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg
      601 agaacagggg catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc
      661 ctgggatcaa agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg
      721 aattgctgcc ctctggttat gtgtgggagg gctaagtcga caatcaacct ctggattaca
      781 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
      841 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
      901 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
      961 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     1021 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     1081 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     1141 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     1201 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     1261 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     1321 gtcggatctc cctttgggcc gcctccccgc ctggtacctt taagaccaat gacttacaag
     1381 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     1441 tcccaacgaa gataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     1501 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     1561 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     1621 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     1681 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     1741 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     1801 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     1861 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     1921 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     1981 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac ttttgcagag
     2041 accaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     2101 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     2161 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     2221 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     2281 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     2341 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     2401 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     2461 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     2521 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     2581 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     2641 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     2701 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     2761 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     2821 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     2881 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     2941 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     3001 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     3061 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     3121 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     3181 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     3241 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     3301 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     3361 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     3421 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     3481 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     3541 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     3601 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     3661 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     3721 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     3781 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     3841 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     3901 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     3961 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     4021 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     4081 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     4141 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     4201 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     4261 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     4321 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     4381 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     4441 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     4501 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     4561 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     4621 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag
     4681 ctgacgcgtg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
     4741 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
     4801 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
     4861 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
     4921 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     4981 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     5041 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     5101 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     5161 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     5221 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     5281 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     5341 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     5401 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     5461 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     5521 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     5581 aaaagtaaga ccaccgcaca gcaagcggcc actgatcttc agacctggag gaggagatat
     5641 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     5701 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     5761 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     5821 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     5881 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     5941 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     6001 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     6061 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     6121 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     6181 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     6241 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     6301 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     6361 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     6421 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     6481 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     6541 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa
     6601 aattttatcg atactagttc caccccacag tggtttactc cctatcagtg atagagaacg
     6661 tatgaagagt ttactcccta tcagtgatag agaacgtatg cagactttac tccctatcag
     6721 tgatagagaa cgtataagga gtttactccc tatcagtgat agagaacgta tgaccagttt
     6781 actccctatc agtgatagag aacgtatcta cagtttactc cctatcagtg atagagaacg
     6841 tatatccagt ttactcccta tcagtgatag agaacgtata agctttaggc gtgtacggtg
     6901 ggcgcctata aaagcagagc tcgtttagtg aaccgtcaga tcgcctggag caattccaca
     6961 acacttttgt cttataccaa ctttccgtac cacttcctac cctcgtaaat ctagaatgcg
     7021 tttcccctac gggcggccag agttcctggg gctgtctcag gacgaggtgg agtgcagcgc
     7081 cgaccacatc gcccgcccca tcctcatcct caaggagact cggcggctgc cctgggccac
     7141 tggctacgca gaggttatca atgccgggaa gagcacacac aatgaagacc aagccagctg
     7201 tgaggtgctc actgtgaaga agaaggcagg ggccgtgacc tcaaccccaa acaggaactc
     7261 atccaagaga cggtcctccc ttcccaatgg ggaagggctg cagctgaagg agaactcgga
     7321 atccgagggt gtttcctgcc actattggtc gctgtttgac gggcacgcgg ggtccggggc
     7381 cgcggtggtg gcgtcacgcc tgctgcagca ccacatcacg gagcagctgc aggacatcgt
     7441 ggacatcctg aagaactccg ccgtcctgcc ccctacctgc ctgggggagg agcctgagaa
     7501 cacgcccgcc aacagccgga ctctgacccg ggcagcctcc ctgcgcggag gggtgggggc
     7561 cccgggctcc cccagcacgc cccccacacg cttctttacc gagaagaaga ttccccatga
     7621 gtgcctggtc atcggagcgc ttgaaagtgc attcaaggaa atggacctac agatagaacg
     7681 agagaggagt tcatataata tatctggtgg ctgcacggcc ctcattgtga tttgcctttt
     7741 ggggaagctg tatgttgcaa atgctgggga tagcagggcc ataatcatca gaaatggaga
     7801 aattatcccc atgtcttcag aatttacccc cgagacggag cgccagcgac ttcagtacct
     7861 ggcattcatg cagcctcact tgctgggaaa tgagttcaca catttggagt ttccaaggag
     7921 agtacagaga aaggagcttg gaaagaagat gctctacagg gactttaata tgacaggctg
     7981 ggcatacaaa accattgagg atgaggactt gaagttcccc cttatatatg gagaaggcaa
     8041 gaaggcccgg gtaatggcaa ctattggagt gaccagggga cttggggacc atgacctgaa
     8101 ggtgcatgac tccaacatct acattaaacc attcctgtct tcagctccag aggtaagaat
     8161 ctacgatctt tcaaaatatg atcatggatc agatgatgtg ctgatcttgg ccactgatgg
     8221 actctgggac gttttatcaa atgaagaagt agcagaagca atcactcagt ttcttcctaa
     8281 ctgtgatcca gatgatcctc acaggtacac actggcagct caggacctgg tgatgcgtgc
     8341 ccggggtgtg ctgaaggaca gaggatggcg gatatctaat gaccgactgg gctcaggaga
     8401 cgacatttct gtatatgtca ttcctttaat acatggaaac aagctgtcat cagcggccgc
     8461 catgcaacgt gccctgccag gcgcccgcca gcacttgggg gccattctgg ccagcgccag
     8521 cgtggtggtg aaggctctgt gtgcggcggt actattcctc tacctgctct ccttcgccgt
     8581 ggacacaggc tgcctggcgg tcaccccggg ctacctcttt cctcccaact tctggatctg
     8641 gaccctggcc acccatgggc tgatggagca gcatgtgtgg gacgtggcca tcagcctgac
     8701 aacggtggtg gtggccgggc gtttgctgga gcccctctgg ggggccttgg agctgctcat
     8761 cttcttctca gtggtgaatg tgtctgtagg gctgctgggg gccttcgcct acctcctcac
     8821 ctacatggct tccttcaacc tggtctacct gttcactgtc cgtatccacg gcgccttggg
     8881 cttcctaggt ggcgtcctgg tggcactcaa gcaaaccatg ggggactgtg tggtcctgcg
     8941 agtgccccag gtgcgcgtca gtgtgatgcc catgctgctg ctggcgctgc tgctcctgct
     9001 gcggctcgcc acgctgctcc agagcccggc gctggcttcc tatggcttcg ggctgctctc
     9061 cagttgggta tatcttcgct tctaccagcg ccatagccgg ggccgagggg acatggctga
     9121 ccactttgct ttcgccactt tcttccctgc tagctaccct tacgacgtgc cagactacgc
     9181 ctaaggccgc aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc
     9241 ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt
     9301 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     9361 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     9421 ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc
     9481 ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg
     9541 cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt
     9601 gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc
     9661 tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc
     9721 tgtgaccggc gcctacgcca ccatgagccg gctggacaag agcaaagtga tcaacagcgc
     9781 cctggaactg ctgaacggcg tgggcatcga gggcctgacc acccggaagc tggcccagaa
     9841 actgggcgtg gaacagccca ccctgtactg gcacgtgaag aacaagcggg ccctgctgga
     9901 cgccctgccc atcgagatgc tggaccggca ccacacccac agctgccctc tggaaggcga
     9961 gagctggcag gacttcctgc ggaacaacgc caagagctac agatgcgccc tgctgagcca
    10021 ccgggacggc gccaaagtgc acctgggcac cagacccacc gagaagcagt acgagacact
    10081 ggaaaaccag ctggccttcc tgtgccagca gggcttcagc ctggaaaacg ccctgtacgc
    10141 cctgagcgcc gtgggccact tcaccctggg ctgcgtgctg gaagaacagg aacacc
//
