LOCUS       sequence-465049-        9497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of HA-tagged human Delta-37-PPM1H.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9497)
  AUTHORS   Wondwossen M Yeshaw , Ayan Adhikari, Claire Y Chiang, Herschel S 
            Dhekne, Paulina S Wawro, Suzanne R Pfeffer
  TITLE     Localization of PPM1H phosphatase tunes Parkinson's disease-linked 
            LRRK2 kinase-mediated Rab GTPase phosphorylation and ciliogenesis
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Oct 31;120(44)
REFERENCE   2  (bases 1 to 9497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2023 Oct 31;120(44)"
COMMENT     Sequence Label: Lenti-TO-Delta-37-HA PPM1H
FEATURES             Location/Qualifiers
     source          1..9497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             8..406
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    413..1001
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(466..486)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(884..895)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1075..1308
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1380..1501
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1417..1436)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1471..1490
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1541..1676
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1709..1725)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1709..1725)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1722..1744)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1733..1749
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1757..1763,1764..1781,1782..1787))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1802..1823
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1940..1957)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2111..2699)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(2870..3661,3662..3730))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3493..3512
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3731..3835)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3903..3921
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3959..3981)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4081..4100
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4294..4316
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4308..4325
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4309..4325
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4340..4566
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4567..4747
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4794..4919
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5412..5645
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5830..5874
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6141..6257
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6290..6308
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6326..6344
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6362..6380
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6398..6416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6434..6452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6470..6488
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6506..6524
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     6569..6593
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             8107..8133
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        8175..8386
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             8399..8667
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             8695..9438
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             join(9448..9497,1..7)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 tggacctatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc
       61 tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag
      121 cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga
      181 actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc
      241 gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct
      301 cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt
      361 tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaagtcg acaatcaacc
      421 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
      481 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
      541 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
      601 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
      661 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
      721 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
      781 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
      841 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
      901 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
      961 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa
     1021 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     1081 ggctaattca ctcccaacga agataagatc tgctttttgc ttgtactggg tctctctggt
     1141 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     1201 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     1261 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     1321 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     1381 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     1441 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     1501 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     1561 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     1621 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga
     1681 cttttgcaga gaccaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc
     1741 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     1801 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     1861 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     1921 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1981 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     2041 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     2101 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     2161 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     2221 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     2281 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     2341 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     2401 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     2461 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2521 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     2581 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2641 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2701 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     2761 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     2821 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     2881 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     2941 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     3001 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     3061 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     3121 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     3181 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     3241 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     3301 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     3361 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     3421 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     3481 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     3541 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     3601 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     3661 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     3721 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     3781 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     3841 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     3901 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     3961 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     4021 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     4081 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     4141 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
     4201 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     4261 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     4321 ccagtgccaa gctgacgcgt gtagtcttat gcaatactct tgtagtcttg caacatggta
     4381 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
     4441 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
     4501 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
     4561 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     4621 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     4681 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     4741 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     4801 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     4861 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     4921 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     4981 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     5041 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     5101 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     5161 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     5221 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cactgatctt cagacctgga
     5281 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     5341 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     5401 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     5461 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     5521 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     5581 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     5641 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     5701 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     5761 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     5821 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     5881 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     5941 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     6001 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
     6061 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
     6121 tctcgacggt atcggttaac ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     6181 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     6241 acaaaattca aaattttatc gatactagtt ccaccccaca gtggtttact ccctatcagt
     6301 gatagagaac gtatgaagag tttactccct atcagtgata gagaacgtat gcagacttta
     6361 ctccctatca gtgatagaga acgtataagg agtttactcc ctatcagtga tagagaacgt
     6421 atgaccagtt tactccctat cagtgataga gaacgtatct acagtttact ccctatcagt
     6481 gatagagaac gtatatccag tttactccct atcagtgata gagaacgtat aagctttagg
     6541 cgtgtacggt gggcgcctat aaaagcagag ctcgtttagt gaaccgtcag atcgcctgga
     6601 gcaattccac aacacttttg tcttatacca actttccgta ccacttccta ccctcgtaaa
     6661 tctagaatgc gtttccccta cgggcggcca gagttcctgg ggctgtctca ggacgaggtg
     6721 gagtgcagcg ccgaccacat cgcccgcccc atcctcatcc tcaaggagac tcggcggctg
     6781 ccctgggcca ctggctacgc agaggttatc aatgccggga agagcacaca caatgaagac
     6841 caagccagct gtgaggtgct cactgtgaag aagaaggcag gggccgtgac ctcaacccca
     6901 aacaggaact catccaagag acggtcctcc cttcccaatg gggaagggct gcagctgaag
     6961 gagaactcgg aatccgaggg tgtttcctgc cactattggt cgctgtttga cgggcacgcg
     7021 gggtccgggg ccgcggtggt ggcgtcacgc ctgctgcagc accacatcac ggagcagctg
     7081 caggacatcg tggacatcct gaagaactcc gccgtcctgc cccctacctg cctgggggag
     7141 gagcctgaga acacgcccgc caacagccgg actctgaccc gggcagcctc cctgcgcgga
     7201 ggggtggggg ccccgggctc ccccagcacg ccccccacac gcttctttac cgagaagaag
     7261 attccccatg agtgcctggt catcggagcg cttgaaagtg cattcaagga aatggaccta
     7321 cagatagaac gagagaggag ttcatataat atatctggtg gctgcacggc cctcattgtg
     7381 atttgccttt tggggaagct gtatgttgca aatgctgggg atagcagggc cataatcatc
     7441 agaaatggag aaattatccc catgtcttca gaatttaccc ccgagacgga gcgccagcga
     7501 cttcagtacc tggcattcat gcagcctcac ttgctgggaa atgagttcac acatttggag
     7561 tttccaagga gagtacagag aaaggagctt ggaaagaaga tgctctacag ggactttaat
     7621 atgacaggct gggcatacaa aaccattgag gatgaggact tgaagttccc ccttatatat
     7681 ggagaaggca agaaggcccg ggtaatggca actattggag tgaccagggg acttggggac
     7741 catgacctga aggtgcatga ctccaacatc tacattaaac cattcctgtc ttcagctcca
     7801 gaggtaagaa tctacgatct ttcaaaatat gatcatggat cagatgatgt gctgatcttg
     7861 gccactgatg gactctggga cgttttatca aatgaagaag tagcagaagc aatcactcag
     7921 tttcttccta actgtgatcc agatgatcct cacaggtaca cactggcagc tcaggacctg
     7981 gtgatgcgtg cccggggtgt gctgaaggac agaggatggc ggatatctaa tgaccgactg
     8041 ggctcaggag acgacatttc tgtatatgtc attcctttaa tacatggaaa caagctgtca
     8101 gctagctacc cttacgacgt gccagactac gcctaaggcc gcaaggatct gcgatcgctc
     8161 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     8221 ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     8281 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     8341 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg
     8401 ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt
     8461 gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt
     8521 aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag
     8581 actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc
     8641 gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc caccatgagc
     8701 cggctggaca agagcaaagt gatcaacagc gccctggaac tgctgaacgg cgtgggcatc
     8761 gagggcctga ccacccggaa gctggcccag aaactgggcg tggaacagcc caccctgtac
     8821 tggcacgtga agaacaagcg ggccctgctg gacgccctgc ccatcgagat gctggaccgg
     8881 caccacaccc acagctgccc tctggaaggc gagagctggc aggacttcct gcggaacaac
     8941 gccaagagct acagatgcgc cctgctgagc caccgggacg gcgccaaagt gcacctgggc
     9001 accagaccca ccgagaagca gtacgagaca ctggaaaacc agctggcctt cctgtgccag
     9061 cagggcttca gcctggaaaa cgccctgtac gccctgagcg ccgtgggcca cttcaccctg
     9121 ggctgcgtgc tggaagaaca ggaacaccag gtcgccaaag aggaaagaga gacacccacc
     9181 accgacagca tgccccccct gctgaagcag gccatcgagc tgttcgacag acagggcgcc
     9241 gagcccgcct tcctgttcgg cctggaactg atcatctgcg gcctcgagaa gcagctgaag
     9301 tgcgagagcg gcggacccac cgacgccctg gacgacttcg acctggacat gctgcccgcc
     9361 gatgccctgg atgattttga tctggatatg ctccctgctg acgctctcga tgacttcgat
     9421 ctcgatatgc tgccaggcgg aagcggagct actaacttca gcctgctgaa gcaggctgga
     9481 gacgtggagg agaaccc
//
